Protein structure-based combinatorial chemistry: Discovery of non-peptide binding elements to Src SH3 domain

Journal of the American Chemical Society

Volumn 118, Issue 1, 1996, Pages 287-288

  Combs, Andrew P ^a   Kapoor, Tarun M ^a   Feng, Sibo ^a   Chen, James K ^a   Daudé Snow, Lygia F ^a   Schreiber, Stuart L ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0030062879     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja953750v     Document Type: Article

References (14)

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12. This procedure generates a complete representation of the sublibraries: C-M-M-M-PLPPLP. C-M-M-PLPPLP, C-M-PLPPLP, C-PLPPLP.
13. The library was initially incubated with the preformed complex of N-terminally biotinylated (via semicarbazide linkage) Src SH3 domain and streptavidin-alkaline phosphatase (SAAP) and then washed extensively. Treatment of the library with substrate for alkaline phosphatase gave varying degrees of color deposition on the beads. The darkest beads (∼350) were physically removed with a syringe, destained. and then reassayed with SAAP alone in order to eliminate false positives. None of the beads were identified as SAAP binders. A final high-stringency assay of all ∼-350 beads was performed with lower concentrations of Src SH3-SAAP in order to identify the highest affinity ligands
14. Madden, D ; Krchnák, W.; Lebl, M. Perspect. Drug Discovery Des. 1994, 2, 269-285.