Determination of glycated hemoglobins in the rat: Comparison between two different chromatographic methods and application in experimental diabetology

Research in Experimental Medicine

Volumn 196, Issue 1, 1996, Pages 9-16

  De Tata, V ^a   Novelli, M ^a   Bombara, M ^a   Masiello, P ^a   Cappuccio, A ^b   Innocenti, B ^b   Bergamini, E ^a  

^a UNIVERSITY OF PISA   (Italy)

^b Lab Analisi Chimico Cliniche M   (Italy)

Author keywords

Affinity chromatography; Diabetes; Glycated hemoglobin; HPLC method; Rat

Indexed keywords

BORONIC ACID DERIVATIVE; GLUCOSE; GLYCOSYLATED HEMOGLOBIN; HEMOGLOBIN A1C;

AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; AUTOANALYSIS; CONTROLLED STUDY; GLUCOSE BLOOD LEVEL; GLYCATION; HEMOGLOBIN DETERMINATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; RAT; STREPTOZOCIN DIABETES;

EID: 0030062121     PISSN: 03009130     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02576824     Document Type: Article

References (15)

1. Blanc MH, Rhie FH, Dunn PJ, Soeldner JS (1981) The determination of glycosylated hemoglobins in rats using high pressure liquid chromatography. Metabolism 30:317-322
2. Bunn HF, Gabbay KH, Gallop PM (1978) The glycosylation of hemoglobin: relevance to diabetes mellitus. Science 200:21-34
3. Datta MC, Gilman JG (1981) Rat hemoglobin heterogeneity: postnatal changes in proportions of multiple components and effects of erythropoietin on marrow cell cultures. Hemoglobin 5:701-714
4. Fluckiger R, Woodtli T, Berger W (1984) Quantitation of glycosylated hemoglobin by boronate affinity chromatography. Diabetes 33:73-76
5. Garrick LM, Sloan RL, Ryan TW, Klonowski TJ, Garrick MD (1978) Primary structure of the major beta-chain of rat hemoglobins. Biochem J 173:321-330
6. Gonen B, Rubenstein A (1979) Glycosylated hemoglobin in diabetes: a reappraisal. Diabetes Care 2:451-453
7. Higgins PJ, Garlick RL, Bunn HF (1982) Glycosylated hemoglobin in human and animal red cells. Diabetes 31:743-748
8. Klenk DC, Hermanson GT, Krohn RI, Fujimoto EK, Mallia AK, Smith PK, England JD, Wiedmeyer HM, Little RR and Goldstein DE (1982) Determination of glycosylated hemoglobin by affinity chromatography: comparison with colorimetric and ion-exchange methods, and effects of common interferences. Clin Chem 28:2088-2094
9. Kondo N, Shibayama Y, Toyomaki Y, Yamamoto M, Ohara H, Nakano K and Ienaga K (1989) Simple method for determination of A 1c-type glycatcd hemoglobin(s) in rats using high performance liquid chromatography. J Pharmacol Toxicol Methods 21:211-221
10. Masoro EJ, Katz MS and McMahan CA (1989) Evidence for the glycation hypothesis of aging from the food restricted rodent model. J Gerontol 44:B20-B22
11. Odetti P, Borgoglio A, De Pascale A, Rolandi R and Adezati L (1990) Prevention of diabetes-increased aging effect on rat collagen-linked fluorescence by aminoguanidine and rutin. Diabetes 39:796-801
12. Shapiro R, McManus MJ, Zalut C and Bunn HF (1980) Sites of nonenzymatic glycosylation of human hemoglobin A. J Biol Chem 255:3120-3127
13. Stein S, Cherian MG, Mazur A (1971) Preparation and properties of six rat hemoglobins. J Biol Chem 246:5287-5293
14. Trivelli LA, Ranney HM, Lai H (1971) Hemoglobin components in patients with diabetes mellitus. N Engl J Med 284:353-357
15. Vialettes B, Vovan L, Simon MC, Lassmann V, Altomare E, Vague P (1982) Kinetics of fast haemoglobin in diabetic rats. Diabetologia 22:264-268