Liquid-chromatographic study of purine metabolism abnormalities in purine nucleoside phosphorylase deficiency

Clinical Chemistry

Volumn 42, Issue 2, 1996, Pages 326-328

  Chantin, Christiane ^a   Bonin, Blandine ^a   Boulieu, Roselyne ^b   Bory, Claude ^a  

^a HÔPITAL DEBROUSSE   (France)

^b Institut des Sciences Pharmaceutiques et Biologiques   (France)

Author keywords

chromatography, anion exchange; chromatography, reversed phase; erythrocytes; guanosine; heritable disorders; urine

Indexed keywords

DEOXYGUANOSINE; DEOXYGUANOSINE PHOSPHATE; DEOXYGUANOSINE TRIPHOSPHATE; DEOXYINOSINE; GUANOSINE; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; INOSINE; PURINE NUCLEOSIDE; PURINE NUCLEOSIDE PHOSPHORYLASE; PURINE NUCLEOTIDE;

ACCURACY; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CASE REPORT; ENZYME ACTIVITY; ENZYME DEFICIENCY; ERYTHROCYTE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; INFANT; MALE; PURINE METABOLISM; REVERSED PHASE LIQUID CHROMATOGRAPHY;

EID: 0030062097     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/42.2.326     Document Type: Article

References (8)

1. Boulieu R, Bory C, Gonnet C. High-performance liquid chromatographic method for the analysis of purine and pyrimidine bases, ribonucleosides and deoxyribonucleosides in biological fluids. J Chromatogr 1985;33:380-7.
2. Boulieu R, Bory C, Gonnet C. Liquid chromatographic method for measuring purine nucleotides in human cells. Clin Chem 1985; 31:727-31.
3. Kim BK, Cha S, Parks RE Jr. Purine nucleoside phosphorylase from human erythrocytes. I. Purification and properties. J Biol Chem 1968;243:1763-70.
4. Simmonds HA, Sahota A, Potter CF, Cameron JS. Purine metabolism and immunodeficiency: urinary purine excretion as a diagnostic screening test in adenosine deaminase and purine nucleoside phosphorylase deficiency. Clin Sci Mol Med 1978;54:579-84.
5. Simmonds HA, Fairbanks LD, Morris GS, Webster DR, Harley EH. Altered erythrocyte nucleotide patterns are characteristic of inherited disorders of purine or pyrimidine metabolism. Clin Chim Acta 1988;171:197-210.
6. Hershfield MS, Mitchell BS. Immunodeficiency diseases caused by adenosine deaminase deficiency and purine nucleoside phosphorylase deficiency. In: Scriver CR, Beaudet AL, Sly WS, Valle D, eds. Metabolic and molecular basis of inherited disease, 7th ed., Vol. 2. New York: McGraw-Hill, 1995:1725-08.
7. Bory C, Boulieu R, Souillet G, Chantin C, Rolland MO, Mathieu M, Hershfield MS. Comparison of red cell transfusion and polyethylene glycol-modified adenosine deaminase therapy in an adenosine deaminase-deficient child: measurement of erythrocyte deoxyadenosine triphosphate as a useful tool. Pediatr Res 1990; 28:127-30.
8. Boulieu R, Bory C, Baltassat P, Gonnet C. Hypoxanthine and xanthine levels determined by high performance liquid chromatography in plasma, erythrocyte and urine samples from healthy subjects: the problem of hypoxanthine level evolution as a function of time. Anal Biochem 1983;129:398-40.