A biotinylated undecylthiophene copolymer bioconjugate for surface immobilization: Creating an alkaline phosphatase chemiluminescence-based biosensor

Bioconjugate Chemistry

Volumn 7, Issue 1, 1996, Pages 159-164

  Pande, Rajiv ^a   Kamtekar, Sanjay ^a   Ayyagari, Madhu S ^a,b   Kamath, Manjunath ^a   Marx, Kenneth A ^a   Kumar, Jayant ^a   Tripathy, Sukant K ^a   Kaplan, David L ^b  

^a University of Massachusetts Lowell   (United States)

^b US Army Natick RD and E Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMILUMINESCENCE; CONJUGATED POLYMERS; ENZYME ACTIVITY; GLASS; HYDROPHOBICITY; BIOSENSORS;

ALKALINE PHOSPHATASE; BIOCONJUGATES; CONJUGATED COPOLYMERS; GLASS CAPILLARIES; GLASS SURFACES; METHYL PARATHION; MOLECULAR ASSEMBLY; PARAOXON; SILANIZED; SURFACE IMMOBILIZATION;

PHOSPHATASES;

BACTERIA (MICROORGANISMS);

EID: 0030061219     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc950086z     Document Type: Article

References (19)

1. Marx, K. A., Samuelson, L. A., Kamath, M., Sengupta, S., Kaplan, D. L., Kumar, J., and Tripathy, S. K. (1994) Intelligent Biomateriats Based on LB Monolayer Films. In Molecular and Biomolecular Electronics (R. R. Birge, Ed.) pp 395-412, Advances in Chemistry Series 240, American Chemical Society Books, Washington, DC.
2. Chaiet, L., and Wolf, F. J. (1964) The properties of Streptavidin, a biotin binding protein produced by Streptomycetes. Arch. Biochem. Biophys. 106, 1.
3. Green, N. M. (1975) Adv. Protein Chem. 29, 85.
4. Langer, P. R., Waldrop, A. A., and Ward, D. C. (1981) Enzymatic synthesis of biotin-labeled polynucleotides; Novel nucleic acid affinity probes. Proc. Natl. Acad. Sci. U.S.A. 78, 6633.
5. Wilchek, M., and Bayer, E. A. (1988) The Avidin-Biotin Complex in Bioanalytical Applications. Anal. Biochem. 171, 1.
6. Samuelson, L. A., Miller, P., Galotti, D. M., Marx, K. A., Kumar, J., Tripathy, S. K., and Kaplan, D. L. (1991) Oriented Fluorescent Streptavidin Conjugated Phycoerythrin Protein on Biotinylated Lipid L-B Monolayer Films. In Proteins Structure, Dynamics and Design (V. Renugopalakrishnan, P. R. Carey, I. C. P. Smith, S. G. Huang, and A. C. Storer, Eds.) pp 160-164, Escom Science Publishers BV, Leiden, The Netherlands.
7. Samuelson, L. A., Kaplan, D. L., Lim, J. O., Kamath, M. N., Marx, K. A., and Tripathy, S. K. (1994) Molecular Recognition Between a Biotinylated Polythiophene Copolymer and Phycoerythrin Utilizing the Biotin-Steptavidin Interaction. Thin Solid Films 242, 796.
8. Burroughs, J. H., Jones, C. A., and Friend, R. H. (1988) New Semiconductor device physics in polymer diodes and transistors. Nature 335, 137.
9. Naarman, H. (1990) The Development of Electrically Conducting Polymers. Adv. Mater. 2, 8, 345.
10. Elsenbaumer, R. L., Jen, K. Y., Miller, G. G., and Shacklette, L. W. (1987) Processable Environmentally stable, Highly Conductive forms of Polythiophene. Synth. Met. 18, 277.
11. Kamath, M., Lim, J. O., Chittibabu, K. G., Sarma, R., Samuelson, L. A., Akkara, J. A., Kaplan, D. L., Marx, K. A., Kumar, J., and Tripathy, S. K. (1993) Biotinylated Poly-(3-Hexylthiophene-co-3-Methanolthiophene): A Langmuir Monolayer-Forming Copolymer. J. M. S. Pure Appl. Chem. A 30 (8), 493.
12. Green, N. M., Konieczny, L., Toms, E. J., and Valentine, R. C. (1971) The use of biotinyl compounds to determine the arrangement of subunits in avidin. Biochem. J. 725, 781.
13. Martin, C., Bresnick, L., Juo, R. R., Voyta, J. C., and Bronstein, I. (1991) Improved Chemiluminescent DNA Se-quencing. BioTechniques 11, 1, 110.
14. Sugimoto, R., Takeda, S., Gu, H. B., and Yoshino, K. (1986) Preparation of soluble polythiophene derivatives transition metal halides as catalysts and their property. Chem. Express 1, 635.
15. The Merck Index (1989) p 1112, Merck & Co. Ltd., Rahway, NJ.
16. Coleman, J. E. (1992) Structure and Mechanism of Alkaline Phosphatase. Annu. Rev. Biophys. Biomol. Struct., 441.
17. Ayyagari, M. S., Pande, R., Kamtekar, S., Marx, K. A., Kumar, J., Tripathy, S. K., Akkara, J. A., and Kaplan, D. L. (1995) Molecular Assembly of Proteins and Conjugated Polymers: Toward Development of Biosensors. Biotechnol. Bioeng. 45, 116.
18. Ayyagari, M. S., Kamtekar, S., Pande, R., Marx, K. A., Kumar, J., Tripathy, S. K., Akkara, J. A., and Kaplan, D. L. (1995) Biotechnol. Prog 11, 699.
19. Tropix catalog (1994) p 8, Tropix Inc., Bedford MA (Sapphire, Sapphire-II, Emerald, Emerald-II, Nitro BlocLuminescence Enhancers.