Role of Rho in chemoattractant-activated leukocyte adhesion through integrins

Science

Volumn 271, Issue 5251, 1996, Pages 981-983

  Laudanna, Carlo ^b   Campbell, James J ^b   Butcher, Eugene C ^b  

^a STANFORD UNIVERSITY   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOATTRACTANT; COMPLEMENT COMPONENT C3; FIBRINOGEN; FORMYLPEPTIDE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR; INTEGRIN; INTERLEUKIN 8 RECEPTOR; RHO FACTOR; TRANSFERASE; VASCULAR CELL ADHESION MOLECULE 1;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA TRANSFECTION; LEUKOCYTE ADHERENCE; LYMPHOID CELL; MOUSE; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL;

EID: 0030059222     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5251.981     Document Type: Article

References (24)

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20. 2 for C3 transferase treatments] and was subtracted from agonist-stimulated adhesion for data presentation.
21. 2, respectively, and was subtracted.
22. 35S] (50 μCi/ml). The cells were syringe-loaded through a tuberculin syringe with a 33-gauge needle (13) After 14 passes through the needle, 0.5 to 1% of added radioactivity was incorporated into the cells. The cells were washed twice and, after 20 min recovery at 37°C, stimulated for 1 min and processed as above. After 3 days of exposure, radioactivity was detected with a Molecular Dynamics Phosphorlmager 445 Sl
23. 35S] (50 μCi/ml). The cells were syringe-loaded through a tuberculin syringe with a 33-gauge needle (13) After 14 passes through the needle, 0.5 to 1% of added radioactivity was incorporated into the cells. The cells were washed twice and, after 20 min recovery at 37°C, stimulated for 1 min and processed as above. After 3 days of exposure, radioactivity was detected with a Molecular Dynamics Phosphorlmager 445 Sl
24. Supported in part by grants from the NIH and by an award from the Department of Veterans Affairs, a fellowship of Dottorato di Ricerca in Biologia e Patoliogia Cellulare e Moflcolare from University of Verona, Verona, Italy (C L.), Cancer, Etiology, Prevention, Detection and Diagnosis training grant 5T32 CA09302, and individual NIH fellowship 1F32 A108930 (J.J C.). We thank A. Hall for GST fusion protein