Determination of dissociation constant and concentration of an anti-DNA antibody by using the light-addressable potentiometric sensor

Journal of Biochemical and Biophysical Methods

Volumn 31, Issue 1-2, 1996, Pages 17-21

  Dill, Kilian ^a   Fraser, Chris ^a   Blomdahl, Jayne A ^a   Olson, John D ^a  

^a MOLECULAR DEVICES CORPORATION   (United States)

Author keywords

Anti DNA antibody; Biotinylated oligonucleotide; Dissociation constant; Light addressable potentiometric sensor

Indexed keywords

DNA ANTIBODY;

BINDING SITE; BIOSENSOR; DNA BINDING; POTENTIOMETRY; PRIORITY JOURNAL; QUANTITATIVE ASSAY; SHORT SURVEY;

EID: 0030058944     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0165-022X(95)00023-K     Document Type: Article

References (7)

1. Dill, K., Lin, M., Poteras, C., Fraser, C., Hafeman, D.G., Owicki, J.C. and Olson, J.D. (1994) Antibody-antigen binding constants determined in solution phase with the Threshold membrane-capture system: binding constants for anti-fluorescein, anti-saxitoxin, and anti-ricin antibodies, Anal. Biochem. 217, 128-138.
2. Panfili, P.R., Dill, K. and Olson, J.D. (1994) Immunochemical detection using the light-addressable potentiometric sensor, Curr. Opin. Biotech. 5, 60-64.
3. Kung, V.T., Panfili, P.R., Sheldon, E.L., King, R.S., Nagainis, P., Gomez, B., Ross, D.A., Briggs, J. and Zuk, R.F. (1990) Picogram quantitation of total DNA using DNA-binding proteins in a silicon sensor-based system. Anal. Biochem. 187, 220-227.
4. Briggs, J. and Panfili, P.R. (1991) Quantitation of DNA and protein impurities in biopharmaceuticals, Anal. Chem. 63, 850-859.
5. Olson, J.D., Panfili, P.R., Zuk, R.F. and Sheldon, E.L. (1991) Quantitation of DNA hybridization in a silicon sensor-based system, Mol. Cell. Probes 5, 351-358.
6. King, R.S. and Panfili, P.R. (1991) Influence of fragment size of DNA using DNA-binding proteins and a sensor-based analytical system: application in the testing of biological products, J. Biochem. Biophys. Methods 23, 83-93.
7. Merrick, H. and Hawlitschek G. (1992) System for quantitative analysis of total DNA, protein impurities and relevant proteins, Biotech Forum Eur. 6, 398-403.