High-level expression of fully active human glutaredoxin (thioltransferase) in E. coli and characterization of Cys7 to Ser mutant protein

FEBS Letters

Volumn 378, Issue 1, 1996, Pages 69-73

  Padilla, C Alicia ^a,b   Spyrou, Giannis ^a   Holmgren, Arne ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b UNIVERSITY OF CÓRDOBA   (Spain)

Author keywords

Fluorescence; Glutaredoxin; Recombinant protein; Site directed mutagenesis; Thio disulfide; Thioltransferase

Indexed keywords

GLUTAREDOXIN; OXIDOREDUCTASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI; EXPRESSION VECTOR; FLUORESCENCE; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; ISOELECTRIC FOCUSING; MUTANT; NORMAL HUMAN; PLACENTA; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECOMBINANT DNA TECHNOLOGY; SEQUENCE ANALYSIS;

ESCHERICHIA COLI;

EID: 0030058359     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/0014-5793(95)01413-6     Document Type: Article

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