Heparin induces apoptosis in human peripheral blood neutrophils

British Journal of Haematology

Volumn 94, Issue 1, 1996, Pages 48-52

  Manaster, J ^a   Chezar, J ^a   Shertz Swirski, R ^a   Shapiro, G ^a   Tendler, Y ^a   Kristal, B ^a   Shasha, S M ^a   Sela, S ^a  

^a WESTERN GALILEE HOSPITAL   (Israel)

Author keywords

Apoptosis; Flow cytometry; Heparan; Neutrophils; Trypan blue exclusion; TUNEL assay

Indexed keywords

HEPARAN SULFATE; HEPARIN; TRYPAN BLUE;

ANTIINFLAMMATORY ACTIVITY; APOPTOSIS; ARTICLE; BLOOD CELL; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; NEUTROPHIL; PRIORITY JOURNAL;

EID: 0030057350     PISSN: 00071048     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2141.1996.6202063.x     Document Type: Article

References (19)

1. Bursch, W., Paffe, S., Putz, B., Barthel, G. & Schulte-Hermann, R. (1990) Determination of the length of the histological stages of apoptosis in normal liver and altered hepatic foci of rats. Carcinogenesis, 11, 847-853.
2. Capecchi, P.L., Ceccatelli, L., Laghi-Pasini, F. & Di-Perri, T. (1993) Inhibition of neutrophil function in vitro by heparan sulfate. International Journal of Tissue Reactions, 15, 71-76.
3. Cohen, G.M., Sun, X.M., Snowden, R.T, Dinsdale, D. & Skilleter, D.N. (1992) Key morphological features of apoptosis may occur in the absence of internucleosomal DNA degradation. Biochemical Journal, 286, 331-334.
4. Darzynkiewicz, Z., Bruno, S., Del Bino, G., Gorczyca, W., Hotz, M.A., Lasotta, P. & Traganos, F. (1992) Features of apoptotic cells measured by flow cytometry. Cytometry, 13, 795-808.
5. Diamond, M.S., Alon, R., Parkos, C.A., Quinn, M.T. & Springer, T.A. (1995) Heparin is an adhesive ligand for the leukocyte integrin Mac-1. Journal of cell Biology, 130, 1473-1482.
6. Fedarko, N.S. Ishihara, M. & Conrad, H.E. (1989) Control of cell division of hepatoma cells by exogenous heparan sulfate proteoglycan. Journal of Cellular Physiology 139, 287-294.
7. Koury, M.J. (1992) Programmed cell death (apoptosis) in hematopoiesis. Experimental Hematology, 20, 391-394.
8. Leculier, C., Benzerara, O., Couprie, N., Francina, A., Lasne, Y., Archimbaud, E. & Fiere, D. (1992) Specific binding between human neutrophils and heparin. British Journal of Haematology, 81, 81-85.
9. Mandal, A.K., Lyden, T.W. & Saklayen, M.G (1995) Heparin lowers blood pressure: biological and clinical perspectives. Kidney International, 47, 1017-1022.
10. Nelson, R.M., Cecconi, O., Roberts, G., Aruffo, A., Linhardt, R.J. & Bevilacqua, M.P. (1993) Heparin oligosaccharides bind L- and P-selectin and inhibit acute inflammation. Blood, 82, 3253-3258.
11. Pillai, S., Gilliam, L., Conrad, H.E. & Holleran. W.B. (1994) Heparin and its non-anticoagulant analogues inhibit human keratinocyte growth without inducing differentiation. Journal of Investigatiive Dermatology, 103, 647-650.
12. Reilly, C.F., Fritze, L.M.S. & Rosenberg, R.D. (1986) Heparin inhibition of smooth muscle cell proliferation: a cellular site of action. Journal of Cellular Physiology, 129, 11-19.
13. Reilly, C.F., Kindy, M.S., Brown, K.E., Rosenberg, R.D. & Sonenshein, G.H. (1989) Heparin prevents vascular smooth muscle cell progression through the G1 phase of the cell cycle. Journal of Biological Chemistry, 264, 6990-6995.
14. Savill, J.S., Wyllie, A.H., Henson, J.E., Walport, M.J., Henson, P.M. & Haslett, C. (1989) Macrophage phagocytosis of aging neutrophils in inflammation. Journal of Clinical Investigation, 83, 865-875.
15. Schlessinger, J., Lax, I. & Lemmon, M. (1995) Regulation of growth factor activation by proteoglycans: what is the role of the low affinity receptors? Cell, 83. 357-360.
16. Squier, M.K.T., Sehnert, A.J. & Cohen, J.J. (1995) Apoptosis in leukocytes. Journal of Leukocyte Biology, 57, 2-10.
17. Telford, W.G., King, L.F. & Fraker, P.J. (1994) Rapid quantitation of apoptosis in pure and heterogeneous cell populations using flow cytometry. Journal of Immunological Methods, 172, 1-16.
18. Wright, T.C., Pukac, L.A., Castellot, J.J., Karnovsky, M.J., Levine, R.A., Kim-Park, H.Y. & Campisi, J. (1989) Heparin suppresses the induction of c-Fos and c-Myc mRNA in murine fibroblasts by selective inhibition of a protein kinase C-dependent pathway. Proceedings of the National Academy of Sciences of the United States of America, 86, 3199-3203.
19. Yang, L. & Yang, Y-C. (1995) Heparin inhibits the expression of interleukin-11 and granulocyte-macrophage colony-stimulating factor in primate bone marrow stromal fibroblasts through mRNA destabilization. Blood, 86, 2526-2533.