Demonstration and characterization of the iron regulatory protein in human brain

Journal of Neurochemistry

Volumn 67, Issue 2, 1996, Pages 838-844

  Hu, Jing ^a   Connor, James R ^a  

^a PENN STATE MILTON S HERSHEY MEDICAL CENTER   (United States)

Author keywords

Brain; Ferritin; Iron; Iron regulatory protein; Iron responsive element; Transferrin receptor

Indexed keywords

5 AMINOLEVULINATE SYNTHASE; BRAIN PROTEIN; COMPLEMENTARY DNA; CYTOPLASM PROTEIN; FERRITIN; IRON REGULATORY FACTOR; RNA; RNA BINDING PROTEIN; TRANSFERRIN RECEPTOR;

ARTICLE; ASTROCYTOMA CELL; BRAIN; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN TISSUE; IRON RESPONSIVE ELEMENT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN RNA BINDING;

EID: 0030057235     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1996.67020838.x     Document Type: Article

References (40)

1. Aziz N. and Munro H. N. (1987) Iron regulates ferritin mRNA translation through a segment of its 5′ untranslated region. Proc. Natl. Acad. Sci. USA 84, 8478-8482.
2. Beard J. L., Connor J. R., and Jones B. C. (1993) Iron in the brain. Nutr. Rev. 51, 157-170.
3. Bettany A. J., Eisenstein R. S., and Munro H. N. (1992) Mutagenesis of the iron-regulatory element further defines a role for RNA secondary structure in the regulation of ferritin and transferrin receptor expression. J. Biol. Chem. 267, 16531-16537.
4. Casey J. L., Koeller D. M., Ramin V. C., Klausner R. D., and Harford J. B. (1989) Iron regulation of transferrin receptor mRNA levels requires iron-responsive elements and a rapid turnover determinant in the 3′ untranslated region of the mRNA. EMBO J. 8, 3693-3699.
5. Chomczynski P. and Sacchi N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
6. Connor J. R. and Menzies S. L. (1995) Cellular management of iron in the brain. J. Neurol. Sci. 134 (Suppl.), 33-44.
7. Connor J. R., Menzies S. L., St. Martin S., and Mufson E. J. (1990) Cellular distribution of transferrin, ferritin, and iron in normal and aged human brains. J. Neurosci. Res. 27, 595-598.
8. Connor J. R., Menzies S. L., St. Martin S. M., and Mufson E. J. (1992) A histochemical study of iron, transferrin, and ferritin in Alzheimer's diseased brains. J. Neurosci. Res. 31, 75-83.
9. Connor J. R., Snyder B. S., Arosio P., Loeffler D. A., and LeWitt P. (1995) A quantitative analysis of isoferritins in select regions of aged, parkinsonian, and Alzheimer's diseased brains. J. Neurochem. 65, 717-724.
10. Cox T. C., Bawden M. J., Martin A., and May B. K. (1991) Human erythroid 5-aminolevulinate synthase: promoter analysis and identification of an iron-responsive element in the mRNA. EMBO J. 10, 1891-1902.
11. Dandekar T., Stripecke R., Gray N. K., Goossen B., Constable A., Johansson H. E., and Hentze M. W. (1991) Identification of a novel iron-responsive element in murine and human erythroid delta-aminolevulinic acid synthase mRNA. EMBO J. 10, 1903-1909.
12. Dickinson T. K. and Connor J. R. (1995) Cellular distribution of iron, transferrin, and ferritin in the hypotransferrinemic (Hp) mouse brain. J. Comp. Neurol. 355, 67-80.
13. Ehmann W. D., Markesbery W. R., Alauddin M., Hossain T. J., and Brubaker E. H. (1986) Brain trace elements in Alzheimer's disease. Neurotoxicology 7, 195-206.
14. Eisenstein R. S., Tuazon P. T., Schalinske K. L., Anderson S. A., and Traugh J. A. (1993) Iron-responsive element-binding protein. Phosphorylation by protein kinase C. J. Biol. Chem. 268, 27363-27370.
15. Good P. F., Olanow C. W., and Perl D. P. (1992) Neuromelanin-containing neurons of the substantia nigra accumulate. Brain Res. 593, 343-346.
16. Gray N. K. and Hentze M. W. (1994) Iron regulatory protein prevents binding of the 43S translation pre-initiation complex to ferritin and eALAS mRNAs. EMBO J. 13, 3882-3891.
17. Guo B., Yu Y., and Leibold E. A. (1994) Iron regulates cytoplasmic levels of a novel iron-responsive element-binding protein without aconitase activity. J. Biol. Chem. 269, 24252-24260.
18. Guo B., Brown F. M., Phillips J. D., Yu Y., and Leibold E. A. (1995a) Characterization and expression of iron regulatory protein 2 (IRP2). Presence of multiple IRP2 transcripts regulated by intracellular iron levels. J. Biol. Chem. 270, 16529-16535.
19. Guo B., Phillips J. D., Yu Y., and Leibold E. A. (1995b) Iron regulates the intracellular degradation of iron regulatory protein 2 by the proteasome. J. Biol. Chem. 270, 21645-21651.
20. Hallgren B. and Sourander P. (1958) The effect of age on non-haem iron in the human brain. J. Neurochem. 3, 41-51.
21. Harrell C. M., McKenzie A. R., Patino M. M., Walden W. E., and Theil E. C. (1991) Ferritin mRNA: interactions of iron regulatory element with translational regulator protein P-90 and the effect on base-paired flanking regions. Proc. Natl. Acad. Sci. USA 88, 4166-4170.
22. Henderson B. R., Seiser C., and Kuhn L. C. (1993) Characterization of a second RNA-binding protein in rodents with specificity for iron-responsive elements. J. Biol. Chem. 268, 27327-27334.
23. Hentze M. W., Caughman S. W., Rouault T. A., Barriocanal J. G., Dancis A., Harford J. B., and Klausner R. D. (1987) Identification of the iron-responsive element for the translational regulation of human ferritin mRNA. Science 238, 1570-1573.
24. Hentze M. W., Rouault T. A., Harford J. B., and Klausner R. D. (1989) Oxidation-reduction and the molecular mechanism of a regulatory RNA-protein interaction. Science 244, 357-359.
25. Hirling H., Emery-Goodman A., Thompson N., Neupert B., Seiser C., and Kuhn L. C. (1992) Expression of active iron regulatory factor from a full-length human cDNA by in vitro transcription/ translation. Nucleic Acids Res. 20, 33-39.
26. Kaptain S., Downey W. E., Tang C., Philpott C., Halle D., Orloff D. G., Harford J. B., Rouault T. A., and Klausner R. D. (1991) A regulated RNA binding protein also possesses aconitase activity. Proc. Natl. Acad. Sci. USA 88, 10109-10113.
27. Klausner R. D., Rouault T. A., and Harford J. B. (1993) Regulating the fate of mRNA: the control of cellular iron metabolism. Cell 72, 19-28.
28. Larkin E. C. and Rao A. (1990) Importance of fetal and neonatal iron: adequacy for normal development of the central nervous system, in Brain, Behaviour, and Iron in the Infant Diet, pp. 43-62. Springer-Verlag, New York.
29. Leibold E. A. and Munro H. N. (1987) Characterization and evolution of the expressed rat ferritin light subunit gene and its pseudogene family. Conservation of sequences within noncoding regions of ferritin genes. J. Biol. Chem. 262, 7335-7341.
30. Leibold E. A. and Munro H. N. (1988) Cytoplasmic protein binds in vitro to a highly conserved sequence in the 5′ untranslated region of ferritin heavy- and light-subunit mRNAs. Proc. Natl. Acad. Sci. USA 85, 2171-2175.
31. Mullner E. W., Neupert B., and Kuhn L. C. (1989) A specific mRNA binding factor regulates the iron-dependent stability of cytoplasmic transferrin receptor mRNA. Cell 58, 373-382.
32. Mullner E. W., Rothenberger S., Muller A. M., and Kuhn L. C. (1992) In vivo and in vitro modulation of the mRNA-binding activity of iron-regulatory factor. Tissue distribution and effects of cell proliferation, iron levels and redox state. Eur. J. Biochem. 208, 597-605.
33. Neupert B., Thompson N. A., Meyer C., and Kuhn L. C. (1990) A high yield affinity purification method for specific RNA-binding proteins: isolation of the iron regulatory factor from human placenta. Nucleic Acids Res. 18, 51-55.
34. Roskams A. J. I. and Connor J. R. (1994) Iron, transferrin, and ferritin in the rat brain during development and aging. J. Neurochem. 63, 709-716.
35. Rouault T. A., Hentze M. W., Haile D. J., Harford J. B., and Klausner R. D. (1989) The iron-responsive element binding protein: a method for the affinity purification of a regulatory RNA-binding protein. Proc. Natl. Acad. Sci. USA 86, 5768-5772.
36. Rouault T. A., Tang C. K., Kaptain S., Burgess W. H., Haile D. J., Samaniego F., McBride O. W., Harford J. B., and Klausner R. D. (1990) Cloning of the cDNA encoding an RNA regulatory protein-the human iron-responsive element-binding protein. Proc. Natl. Acad. Sci. USA 87, 7958-7962.
37. Samaniego F., Chin J., Iwai K., Rouault T. A., and Klausner R. D. (1994) Molecular characterization of a second iron-responsive element binding protein, iron regulatory protein 2. Structure, function, and post-translational regulation. J. Biol. Chem. 269, 30904-30910.
38. Schneider C., Owen M. J., Banville D., and Williams J. G. (1984) Primary structure of human transferrin receptor deduced from the mRNA sequence. Nature 311, 675-678.
39. Theil E. C. (1994) Iron regulatory elements (IREs): a family of mRNA non-coding sequences. Biochem. J. 304, 1-11.
40. Zheng L., Kennedy M. C., Blondin G. A., Beinert H., and Zalkin H. (1992) Binding of cytosolic aconitase to the iron responsive element of porcine mitochondrial aconitase mRNA. Arch. Biochem. Biophys. 299, 356-360.