Long-term performance of albumin secretion of hepatocytes cultured in a packed-bed reactor utilizing porous resin

Artificial Organs

Volumn 20, Issue 7, 1996, Pages 803-807

  Miyoshi, Hirotoshi ^a,b   Yanagi, Kennichi ^a   Fukuda, Hideki ^c   Ohshima, Norio ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

^b KANEKA CORPORATION   (Japan)

^c KOBE UNIVERSITY   (Japan)

Author keywords

Albumin; Artificial liver; Hepatocytes; Long term culture; Packed bed reactor; Porous resin

Indexed keywords

ARTIFICIAL ORGANS; BODY FLUIDS; CHEMICAL REACTORS; MONOLAYERS; PHYSIOLOGY; RESINS;

ALBUMIN; ALBUMIN SECRETION; ARTIFICIAL LIVER; HEPATOCYTES; LONG TERM PERFORMANCE; LONG-TERM CULTURE; MONOLAYER CULTURE; PERFUSION CULTURES; POLYVINYL FORMALS; POROUS RESIN;

PACKED BEDS;

ALBUMIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; LIVER CELL; MALE; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALIA;

EID: 0030055739     PISSN: 0160564X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1525-1594.1996.tb04545.x     Document Type: Article

References (12)

1. Yanagi K, Miyoshi H, Fukuda H, Ohshima N. A packed-bed reactor utilizing porous resin enables high density culture of hepatocytes. Appl Microbiol Biotechnol 1992;37:316-20.
2. Miyoshi H, Yanagi K, Fukuda H, Ohshima N. Long-term continuous culture of hepatocytes in a packed-bed reactor utilizing porous resin. Biotechnol Bioeng 1994;43:635-44.
3. Rozga J, Holzman MD, Ro M-S, Griffin DW, Neuzil DF, Giorgio T, Moscioni AD, Demetriou AA. Development of a hybrid bioartificial liver. Ann Surg 1993;217:502-11.
4. Sussman NL, Kelly JH. Improved liver function following treatment with an extracorporeal liver assist device. Artif Organs 1993;17:27-30.
5. Isom HC, Secott T, Georgoff I, Woodworth C, Mummaw J. Maintenance of differentiated rat hepatocytes in primary culture. Proc Natl Acad Sci USA 1985;82:3252-6.
6. Dich J, Vind C, Grunnet N. Long-term culture of hepatocytes: Effect of hormones on enzyme activities and metabolic capacity. Hepatology 1988;8:39-45.
7. Landry J, Bernier D, Ouellet C, Goyette R, Marceau N. Spheroidal aggregate culture of rat liver cells: Histotypic reorganization, biomatrix deposition, and maintenance of functional activities. J Cell Biol 1985;101:914-23.
8. Rojkind M, Gatmaitan Z, Mackensen S, Giambrone M-A, Ponce P, Reid LM. Connective tissue biomatrix: Its isolation and utilization for long-term cultures of normal rat hepatocytes, J Cell Biol 1980;87:255-63.
9. Dunn JCY, Tompkins RG, Yarmush ML. Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotechnol Prog 1991;7:237-45.
10. Bissel DM, Arenson DM, Maher JJ, Roll FJ. Support of cultured hepatocytes by a laminin-rich gel: Evidence for a functionally significant subendothelial matrix in normal rat liver. J Clin Invest 1987;79:801-12.
11. Guguen-Guillouzo C, Clément B, Baffet G, Beaumont C, Morel-Chany E, Glaise D, Guillouzo A. Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type. Exp Cell Res 1983;143:47-54.
12. Ohshima N, Shiota M, Kusano H, Wada G, Tsunetsugu T, Ookawa K, Yanagi K. Kinetic analyses of the performance of a hybrid-type artificial liver support system utilizing isolated hepatocytes. Mater Sci Eng 1994;C1:79-85.