An essential lysine in the substrate-binding site of ornithine carbamoyltransferase

European Journal of Biochemistry

Volumn 239, Issue 2, 1996, Pages 397-402

  Valentini, Giovanna ^a   De Gregorio, Ambra ^b   Di Salvo, Cinzia ^b   Grimm, Rudi ^c   Bellocco, Ersilia ^b   Cuzzocrea, Giovanni ^b   Iadarola, Paolo ^a  

^a UNIVERSITY OF PAVIA   (Italy)

^b UNIVERSITY OF MESSINA   (Italy)

^c Hewlett Packard GmbH   (Germany)

Author keywords

Affinity labeling; Carbamoyl phosphate binding site; Ornithine carbamoyltransferase

Indexed keywords

NORVALINE; ORNITHINE CARBAMOYLTRANSFERASE; PYRIDOXAL 5 PHOSPHATE;

AFFINITY LABELING; AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; BINDING SITE; CHEMICAL MODIFICATION; DOLPHIN; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION;

ANIMALIA; FELIS CATUS;

EID: 0030055050     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0397u.x     Document Type: Article

References (26)

1. Bencini, D. A., Houghton, J. E., Hoover, T. A., Foltermann, K. F., Wild, J. R. & O'Donovan, G. A. (1983) The DNA sequence of ArgI from Escherichia coli K12, Nucleic Acids Res. 11, 8509-8518.
2. Ceriotti, G. & Gazzaniga, A. (1966) La determinazione della ornitina carbamil transferasi serica nella diagnosi della epatite virale. Rapido micro ed ultra micro-metodo, Clin. Chim. Acta 14, 57-62.
3. De Gregorio, A., Valentini, G., Bellocco, E., Desideri, A. & Cuzzocrea, G. (1993) A comparative study on liver ornithine carbamoyltransferase from a marine mammal Stenella and an elasmobranch Sphyrna zygaena, Comp. Biochem. Physiol. B 105, 497-501.
4. Eisenstein, E. & Hensley, P. (1986) Ligand-binding promoted conformational changes in yeast ornithine transcarbamoylase, J. Biol. Chem. 261, 6192-6200.
5. Fortin, A. F., Hauber, J. M. & Kantrowitz, E. R. (1981) Comparison of the essential arginine residue in Escherichia coli ornithine and aspartate transcarbamylases, Biochim. Biophys. Acta 662, 8-14.
6. Goldsmith, J. O., Lee, S., Zambidis, I. & Kuo, L. C. (1991) Control of L-ornithine specificity in Escherichia coli ornithine transcarbamoylase, J. Biol. Chem. 266, 18626-18634.
7. Horwich, A. L., Fenton, W. A., Williams, K. R., Kalousec, F., Kraus, J. P., Doolittle, R. F., Konigsberg, W. & Rosenberg, L. E. (1984) Structure and expression of a complementary DNA for the nuclear coded precursor of human mithocondrial ornithine transcarbamylase, Science 224, 1068-1074.
8. Koger, J. B., Howell, R. G., Kelly, M. & Jones, E. E. (1994) Purification and properties of porcine liver ornithine transcarbamylase, Arch. Biochem. Biophys. 309, 293-299.
9. Kuo, L. C., Miller, A. W., Lee, S. & Kozuma, C. (1988) Site-directed mutagenesis of Escherichia coli ornithine transcarbamoylase: role of arginine-57 in substrate binding and catalysis, Biochemistry 27, 8823-8832.
10. Legrain, C., Halleux, P., Stalon, V. & Glansdorff, N. (1972) The dual genetic control of ornithine carbamoyltransferase in Escherichia coli, Eur. J. Biochem. 27, 93-102.
11. Legrain, C. & Stalon, V. (1976) Ornithine carbamoyltransferase from Escherichia coli W, Eur. J. Biochem. 63, 289-301.
12. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193, 265-275.
13. Marshall, M. & Cohen, P. P. (1972) Ornithine transcarbamylase from Streptococcus faecalis and bovine liver, J. Biol. Chem. 247, 1654-1668.
14. Marshall, M. & Cohen, P. P. (1977) Kinetics and equilibrium of the inactivation of ornithine transcarbamylases by pyridoxal 5′-phosphate, J. Biol. Chem. 252, 4276-4286.
15. Marshall, M. & Cohen, P. P. (1980a) Evidence for an exceptionally reactive arginyl residue at the binding site for carbamyl phosphate in bovine ornithine transcarbamylase, J. Biol. Chem. 255, 7301-7305.
16. Marshall, M. & Cohen, P. P. (1980b) The essential sulphydryl group of ornithine transcarbamylases, J. Biol. Chem. 255, 7296-7300.
17. Miller, A. W. & Kuo, L. C. (1990) Ligand-induced isomerizations of Escherichia coli ornithine transcarbamoylase, J. Biol. Chem. 265, 15023-15027.
18. Ruepp, A., Muller, H. N., Lottspeich, F. & Soppa, J. (1995) Catabolic ornithine transcarbamylase of Halobacterium halobium (salinarium): purification, characterization sequence determination, and evolution, J. Bacteriol. 177, 1129-1136.
19. Schachman, H. K., Panza, C. D., Navze, M., Karels, M. J., Wu, L. & Yang, Y. R. (1984) Location of amino acid alterations in mutants of aspartate transcarbamoylase: structural aspects of metallic complementation, Proc. Natl Acad. Sci. USA 81, 115-119.
20. Shen, W. H. (1993) Fluorescence lifetimes of the tryptophan residues in ornithine transcarbamoylase, Biochemistiy 32, 13 925-13 932.
21. Silverman, R. B. (1995) Mechanism-based enzyme inactivators, Methods Enzymol. 249, 240-283.
22. Swenson, R. P., Williams, C. H. Jr, Massey, V., Ronchi, S., Minchiotti, L., Galliano, M. & Curti, B. (1982) The primary structure of D-aminoacid oxidase from pig kidney, I-isolation and sequence of the tryptic peptides, J. Biol. Chem. 257, 8817-8823.
23. Valentini, G., Speranza, M. L., Iadarola, P., Ferri, G. & Malcovati, M. (1988) Reactivity of the fructose 1,6-bisphosphate-activated pyruvate kinase from Escherichia coli with pyridoxal 5′-phosphate, Biol. Chem. Hoppe-Seyler 369, 1219-1226.
24. Valinger, Z., Engel, P. C. & Metzler, D. E. (1993) Is pyridoxal 5′-phosphate an affinity label for phosphate-binding sites in proteins?: the case of glutamate dehydrogenase, Biochem. J. 294, 835-839.
25. Wargnies, B., Legrain, C. & Stalon, V. (1978) Anabolic ornithine carbamoyltransferase of Escherichia coli and catabolic ornithine carbamoyltransferase of Pseudomonas putida, Eur. J. Biochem. 89, 203-212.
26. Zambidis, I. & Kuo, L. (1990) Substrate specificity and protonation state of Escherichia coli ornithine transcarbamoylase as determined by pH studies, J. Biol. Chem. 265, 2620-2623.