Bispecific antibody modification of nicotinic acetylcholine receptors for biosensing

Biosensors and Bioelectronics

Volumn 11, Issue 1-2, 1996, Pages 91-102

  Reiken, Steven R ^a   Van Wie, Bernard J ^a   Sutisna, Himawan ^a   Moffett, David F ^b   Koch, Alan R ^b   Silber, Moris ^b   Davis, William C ^b  

^a Washington State University   (United States)

^b WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

Bilayer lipid membrane; Biosensors; Bispecific antibody; Immunoglobulin; Neuron; Nicotinic acetylcholine receptor; Receptor

Indexed keywords

ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; IMMUNOLOGY; LIPIDS; MEMBRANES; MOLECULES;

BILAYER LIPID MEMBRANE; BIOSENSING; BISPECIFIC ANTIBODY; IMMUNOGLOBULIN; NICOTINIC ACETYLCHOLINE RECEPTORS;

BIOSENSORS;

ANTIBODY; IMMUNOGLOBULIN; NICOTINIC RECEPTOR;

ARTICLE; BIOSENSOR; LIPID MEMBRANE; NERVE CELL;

EID: 0030054326     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/0956-5663(96)83716-X     Document Type: Article

References (24)

1. Anholt, R., Lindstrom, J. & Montal, M. (1981). Stabilization of acetylcholine receptor channels by lipids in cholate solution and during reconstitution in vesicles. J. Biol. Chem., 257, 7122-7134.
2. Anholt, R., Lindstrom, J. & Montal, M. (1984). The molecular basis of neurotransmission: Structure and function of the nicotinic acetylcholine receptor. In: The Enzymes of Biological Membranes. A. Martonosi (ed.), Plenum Press, NY, pp. 335-401.
3. Blatt, Y., Montal, M.S., Lindstrom, J.M. & Montal, M. (1986). Monoclonal antibodies specific to the b and g subunits of the Torpedo acetylcholine receptor inhibit single-channel activity. J. Neurosci., 6, 481-486.
4. Bradford, M.M. (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
5. Brennan, M., Davison, P.F. & Paulus, H. (1985). Preparation of bispecific antibodies by chemical recombination. Science, 229, 81-83.
6. Chang, H.W. & Block, (1977). Molecular forms of the acetylcholine receptor: Effects of calcium ions and sulfhydryl reagent on the occurrence of oligomers. Biochemistry, 16, 4513-4520.
7. Eray, M., Dogan, N.S., Liu, L., Koch, A.R., Moffett, D.F., Silber, M. & Van Wie, B.J. (1994). Highly stable bilayer lipid membranes (BLMs) formed on microfabricated polyimide apertures and their electrical characterization. Biosensors & Bioelectronics, 9, 343-351.
8. Fanger, M.W., Segal, D.M. & Romet-Lemonne, J.-L. (1991). Bispecific antibodies and target cellular cytotoxicity. Immunology Today, 12, 51-54.
9. 2 antibody for the delivery of saporin in the treatment of lymphoma. J. Immunology, 141, 3662-3670.
10. Guesdon, J.L., Velarde, F.N. & Avrameas, S. (1983). Solid-phase immunoassays using chimera antibodies prepared with monoclonal or polyclonal anti-enzyme and anti-erythrocyte antibodies. Ann. Immunol. (Inst. Pasteur). 134C, 265-274.
11. Hamilton, S.L., McLaughlin, M. & Karlin, A. (1977). Disulfide bond cross-linked dimer in acetylcholine receptor from Torpedo californica. Biochem. Biophys. Res. Commun., 79, 692-699.
12. Hess, G.P., Cash, D.J. & Aoshima, H. (1983). Acetylcholine receptor-controlled ion translocation: Chemical kinetic investigations of the mechanism. Ann. Rev. Biophys. Bioeng., 12, 442-473.
13. Kagawa, Y. & Racker, E. (1971). Partial resolution of the enzymes catalyzing oxidative phosphorylation. J. Biol. Chem., 246, 5477-5487.
14. Kisaalita, W.S., Van Wie, B.J., Skeen, R.S., Davis, W.C., Barnes, C.D., Fung, S.J., Chun, K. & Dogan, N.S. (1989). Initiating crossdisciplinary research: the neuron-based chemical sensor project. Chemical Engineering Education, 23, 242-249.
15. Kurdikar, D.L., Skeen, R.S. & Van Wie, B.J. (1992). Temperature effects on neuron-based chemical sensing in well-mixed flow environments. Analytica Chimica Acta, 262, 1-12.
16. Labarca, P., Lindstrom, J. & Montal, M. (1984). Acetylcholine receptor in planar lipid bilayers: Characterization of the channel properties of the purified nicotine acetylcholine receptor from Torpedo californica reconstituted in planar lipid bilayers. J. Gen. Physiol., 83, 473-496.
17. Lindstrom, J., Anholt, R., Einarson, B., Engel, A., Osame, M. & Montal, M. (1980). Purification of acetylcholine receptors, reconstitution into lipid vesicles, and study of agonist-induced cation channel regulation. J. Biol. Chem., 255, 8340-8350.
18. Montal, M., Anholt, R. & Labarca, P. (1986). The reconstituted acetylcholine receptor. In: Ion Channel Reconstitution. C. Miller (ed.), p. 187.
19. Montal, M., LaBarca, P., Fredkin, D.R., Suarez-Isla, B.A. & Lindstrom, J. (1984). Channel properties of the purified acetylcholine receptor from Torpedo Californica reconstituted in planar lipid bilayer membranes. Biophys. J., 45, 165-174.
20. Paulus, H. (1985). Preparation and biomedical applications of bispecific antibodies. Behring Inst. Mitt., 78, 118-132.
21. Schweikl, H., Klein, U., Schindlbeck, M. & Wieczorek, H. (1989). A vacuolar-type ATPase, partially purified from potassium transporting plasma membranes of tobacco hornworm midgut. J. Biol. Chem., 264, 11,136-11,142.
22. Skeen, R.S., Van Wie, B.J., Fung, S.J. & Barnes, C.D. (1992). Effects of temperature and analyte application technique on neuron-based chemical sensing. Biosensors & Bioelectronics, 7, 91-101.
23. Sutisna, H. (1992). Toward a Nicotinic Acetylcholine Receptor-Antibody Based Biosensor. M.S. Thesis, Washington State University, pp. 54-103.
24. Wan, K. & Lindstrom, J. (1985). Effects of monoclonal antibodies on the function of acetylcholine receptors from Torpedo californica and reconstituted into vesicles. Biochemistry, 24, 1212-1221.