The human foamy virus pol gene is expressed as a pro-pol polyprotein and not as a Gag-Pol fusion protein

Journal of Virology

Volumn 70, Issue 2, 1996, Pages 1033-1040

  Lochelt, Martin ^a   Flügel, Rolf M ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GAG PROTEIN; GENE PRODUCT; HYBRID PROTEIN; POL PROTEIN; VIRUS DNA; VIRUS PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CELL STRAIN BHK; ENZYME ACTIVITY; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RETROVIRUS; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION INITIATION; VIRAL GENETICS; VIRUS INFECTIVITY;

EID: 0030053661     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.2.1033-1040.1996     Document Type: Article

References (43)

1. Achong, B. G., P. W. A. Mansell, M. A. Epstein, and P. Clifford. 1971. An unusual virus in cultures from a human nasopharyngeal carcinoma. J. Natl. Cancer Inst. 46:299-307.
2. Aguzzi, A. 1993 The loamy virus family, molecular biology, epidemiology and neuropathology Biochim. Biophys. Acta 1155:1-24.
3. Aronoff, R., A. M. Hajjar, and M. L. Linial. 1993. Avian retroviral RNA encapsidation: reexamination of functional 5′ RNA sequences and the role of nucleocapsid Cys-His motifs. J. Virol. 67:178-188.
4. Bartholomá, A., W. Muranyi, and R. M. Flügel. 1992 Bacterial expression of the capsid antigen domain and identification of native gag proteins in spumavirus-infected cells. Virus Res. 23:27-38.
5. Berlioz, C., and J.-L. Darlix. 1995. An internal ribosomal entry mechanism promotes translation of murine leukemia virus gag polyprotein precursors. J. Virol. 69:2214-2222.
6. Chamorro, M., N. Parkin, and H. E. Varmus. 1992. An RNA pseudoknot and an optimal heptameric shift site are required for highly efficient ribosomal frameshifting on a retroviral messenger RNA. Proc. Natl Acad. Sci. USA 89:713-717.
7. Chang, L.-J., D. Ganem, and H. E. Varmus. 1990. Mechanism of translation of the hepadnaviral polymerase (P) gene. Proc. Natl. Acad. Sci. USA 87: 5158-5162.
8. Chang, L.-J., P. Pryciak, D. Ganem, and H. E. Varmus. 1989 Biosynthesis of the reverse transcriptase of hepatitis B viruses involves de novo translational initiation not ribosomal frameshifting. Nature (London) 337:364-368.
9. Coffin, J. M. 1990. Replication of retroviridae, p. 1437-1500. In B. N. Fields and D. M. Knipe (ed.), Virology. Raven Press, New York.
10. Cullen, B. R. 1991 Human immunodeficiency virus as a prototypic complex retrovirus J. Virol. 65:1053-1056.
11. Farabough, P. J. 1995. Post-transcriptional regulation of transposition by Ty retrotransposons of Saccharomyces cerevisiae. J. Biol. Chem. 270:10361-10364
12. Flügel, R. M., A. Rethwilm, B. Maurer, and G. Darai. 1987. Nucleotide sequence analysis of the env gene and its flanking regions of the human spumaretrovirus reveals two novel genes. EMBO J. 6:2077-2084.
13. Fouillot, N., S. Tlouzeau, J.-M. Rossignol, and O. Jean-Jean. 1993. Translation of the hepatitis B virus P gene by ribosomal scanning as an alternative to internal initiation. J. Virol. 67:4886-4895
14. Fütterer, J., Z. Kiss-Laslo, and T. Hohn. 1993 Nonlinear ribosome migration on cauliflower mosaic virus 35S RNA. Cell 73:789-802.
15. Gupta, K. C., E. Ono, E. V. Aritzia, and M. Inaba. 1994. Translation initiation from non-AUG codons in COS1 cells is mRNA species dependent. Biochem. Biophys Res Commun 201:567-573.
16. Jacks, T. 1990. Translational suppression in gene expression in retroviruses and retrotransposons. Curr. Top. Microbiol. Immunol. 157:93-124
17. Kögel, D., M. Aboud, and R. M. Flügel. 1995. Mutational analysis of the reverse transcriptase and ribonuclease H domains of the human foamy virus. Nucleic Acids Res. 23:2621-2625
18. Kogel, D., M. Aboud, and R. M. Flügel. 1995. Molecularbiological characterization of the human foamy virus reverse transcriptase and ribonuclease H domains. Virology 213:97-105
19. Konvalinka, J., M. Löchelt, H. Zentgraf, R. M. Flügel, and H.-G. Kräusslich. 1995. Active spumavirus proteinase is essential for virus infectivity hut not for formation of the Pol polyprotein. J. Virol. 69:7264-7268.
20. Kozak, M. 1991 Structural features in eukaryotic mRNAs that modulate the initiation of translation. J. Biol Chem. 266:19867-19870
21. Lee, S. I., J. G. Umen, and H. E. Varmus. 1995. A genetic screen identifies cellular factors involved in retroviral - 1 frameshifting. Proc. Natl. Acad Sci. USA 92:6587-6591.
22. Lin, C.-G., and S. J. Lo. 1992. Evidence for involvement of a ribosomal leaky scanning machanism in the translation of the hepatitis B virus Pol gene from the viral pregenome RNA. Virology 188:342-352
23. 22a. Löchelt, M. Unpublished observations.
24. Löchelt, M., and R. M. Flügel. 1995. The molecular biology of primate spumaviruses, p. 239-292. In J. A. Levy (ed.). The Retroviridae, vol. 4, Plenum Press, New York
25. Löchelt, M., R. M. Flügel, and M. Aboud. 1994. The human foamy virus internal promoter directs the expression of the functional Bel 1 transactivator and Bet protein early after infection. J. Virol. 68:638-645.
26. Löchelt, M., W. Muranyi, and R. M. Flugel. 1993. Human foamy virus genome possesses an internal. Bel 1-dependent and functional promoter. Proc. Natl. Acad. Sci. USA 90:7317-7321.
27. Löchelt, M., S. F. Yu, M. L. Linial, and R. M. Flügel. 1995. The human foamy virus internal promoter is required for efficient gene expression and infectivity. Virology 206:601-610.
28. Löchelt, M., H. Zentgraf, and R. M. Flügel. 1991. Construction of an infectious DNA clone of the full-length human spumaretrovirus genome and mutagenesis of the bel 1 gene. Virology 184:43-54.
29. Mahnke, C., M. Löchelt, B. Bannert, and R. M. Flügel. 1990 Specific enzyme-linked immunosorbent assay for the detection of antibodies to the human spumavirus. J. Virol. Methods 29:13-22.
30. Matsufuji, S., T. Matsufuji, Y. Miyazaki, Y. Murakami, J. F. Atkins, R. F. Gesteland, and S.-I. Hayashi. 1995. Autoregulatory frameshifting in decoding mammalian ornithine decarboxylase antizyme. Cell 80:51-60.
31. Maurer, B., H. Bannert, G. Darai, and R. M. Flügel. 1988. Analysis of the primary structure of the long terminal repeat and the gag and pol genes of the human spumaretrovirus. J. Virol. 62:1590-1597.
32. Mergia, A., and P. Luciw. 1991. Replication and regulation of primate foamy viruses. Virology 184:475-482.
33. Mergia, A., K. E. S. Shaw, E. Pratt-Lowe, P. E. Barry, and P. A. Luciw. 1991. Identification of the simian foamy virus transactivator gene (taf). J Virol. 65:2903-2909.
34. Merrick, W. C. 1992 Mechanism and regulation of eukaryotic protein synthesis. Microbiol Rev 56:291-315
35. Muranyi, W., and R. M. Flugel. 1991. Analysis of splicing patterns of human spumaretrovirus by polymerase chain reaction reveals complex RNA structures. J. Virol 65:727-735.
36. Pahl, A., and R. M. Flügel. 1993. Endonucleolytic cleavages and DNA-joining activities of the integration protein of human foamy virus. J Virol 67:5426-5434.
37. Rothnie, H. M., Y. Chapdelaine, and T. Hohn. 1994. Pararetroviruses and retroviruses: a comparative review of viral structure and gene expression strategies Adv Virus Res. 44:1-67.
38. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. 2nd ed Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
39. Schlicht, H.-J., G. Radziwill, and H. Schaller. 1989. Synthesis and encapsidation of duck hepatitis B virus reverse transcriptase do not require formation of core-polymerase fusion protein. Cell 56:85-92
40. Schliephake, A. W., and A. Rethwilm. 1994 Nuclear localization of foamy virus Gag precursor protein. J. Virol. 68:4946-4954.
41. Skalka, A. M. 1989. Retroviral proteases: first glimpses at the anatomy of a processing machine. Cell 56:911-913.
42. Vagner, S., A. Waysbort, M. Marenda, M.-C. Gensac, F. Amalric, and A.-C. Prats. 1995 Alternative translational initiation of the Moloney murine leukemia virus mRNA controlled by internal ribosomal entry involving the p57/PTB splicing factor. J. Biol. Chem. 270:20376-20383
43. Yu, S. F., and M. L. Linial. 1993 Analysis of the role of the bel and bel open reading frames of human foamy virus by using a new quantitative assay J. Virol. 67:6618-6624.