Binding kinetics of an antibody against HIV p24 core protein measured with real-time biomolecular interaction analysis suggest a slow conformational change in antigen p24

Journal of Immunological Methods

Volumn 189, Issue 1, 1996, Pages 1-14

  Glaser, Ralf W ^a,b   Hausdorf, Gert ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

^b ETH ZURICH   (Switzerland)

Author keywords

Binding kinetics; Biomolecular interaction analysis; Conformational change; HIV p24

Indexed keywords

CORE PROTEIN; GAG PROTEIN; IMMUNOGLOBULIN F(AB) PROTEIN; MONOCLONAL ANTIBODY; UNCLASSIFIED DRUG;

ARTICLE; BINDING KINETICS; HUMAN IMMUNODEFICIENCY VIRUS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION;

HUMAN IMMUNODEFICIENCY VIRUS; MURINAE;

EID: 0030053263     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(95)00221-9     Document Type: Article

References (22)

1. Argos, P. (1989) A possible homology between immunodeficiency virus p24 core protein and picornaviral VP2 coat protein: prediction of HIV p24 antigenic sites. EMBO J. 8, 779-785.
2. Franke, L., Grunow, R., Meissner, K., Porstmann, T. and Von Baehr, R. (1992) Inhibition of HIV-1 infection in vitro by murine monoclonal anti-p24 antibodies. J. Med. Virol. 37, 137-142.
3. Friguet, B., Djavadi-Ohaniance, L. and Goldberg, M.E. (1984) Some monoclonal antibodies raised with a native protein bind preferentially to the denatured antigen. Mol. Immunol. 21, 673-677.
4. Friguet, B., Chaffotte, A.F., Djavadi-Ohaniance, L. and Goldberg, M.E. (1985) Measurement of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J. Immunol. Methods 77, 305-319.
5. Friguet, B., Djavadi-Ohaninace, L. and Goldberg, M.E. (1989) Polypeptide-antibody binding mechanism: conformational adaptation investigated by equilibrium and kinetic analysis. Res. Immunol. 140, 355-376.
6. Glaser, R.W. (1993) Antigen-antibody binding and mass transport by convection and diffusion to a surface. Analyt. Biochem. 213, 152-161.
7. Grunow, R., Giese, R., Porstmann, T., Doepel, H., Haensel, K. and von Baehr, R. (1990) Development and biological testing of human and murine monoclonal antibodies against HIV antigens. Z. Klin. Med. 45, 367-369.
8. Hausdorf, G., Gewieß, A., Wray, V. and Porstmann, T. (1994) A recombinant human immunodeficiency virus type-1 capsid protein (rp24): its expression, purification and physico-chemical characterization. J. Virol. Methods 50, 1-9.
9. Hirschberg, B.T. and Schimerlik, M.I. (1994) A kinetic model for oxotremorine m binding to recombinant porcine M2 muscarinic receptors expressed in Chinese hamster ovary cells. J. Biol. Chem. 269, 26127-26135.
10. Hodgson, J. (1994) Light, Angles, Action. Biotechnology 12, 31-35.
11. Höhne, W.E., Küttner, G., Kiessig, S., Hausdorf, G., Grunow, R., Winkler, K., Wessner, H., Giessmann, E., Stiegler, R., Schneider-Mergener, J., Von Baehr, R. and Schomburg, D. (1993) Structural base of the interaction of a monoclonal antibody against p24 of HIV with its peptide epitope. Mol. Immunol. 30, 1213-1221.
12. Huber, W., Hurst, J., Schlatter, D., Barner, R., Hubscher, J., Kouns, W.C. and Steiner, B. (1995) Determination of kinetic constants for the interaction between the platelet glycoprotein IIb-IIIa and fibrinogen by means of surface plasmon resonance. Eur. J. Biochem. 227, 647-655.
13. Johnsson, B., Löfas, S. and Lindquist, G. (1991) Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors. Anal. Biochem. 198, 268-277.
14. Jönsson, U., Fängerstam, L., Ivarsson, B., Johnsson, B., Karlsson, R., Lundh, K., Löfas, S., Persson, B., Roos, H., Rönnberg, I., Sjölander, S., Stenberg, E., Stahlberg, R., Urbaniczky, C., Östlin, H. and Malmquist, M. (1991) Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology. BioTechniques 11, 620-627.
15. Karlsson, R., Michaelsson, A. and Mattsson, L. (1991) Kinetic analysis of monoclonal antibody-antigen interaction with a new biosensor based analytical system. J. Immunol. Methods 145, 229-240.
16. Karlsson, R., Roos, H., Fägerstam, L. and Persson, B. (1994) Kinetic and concentration analysis using BIA technology. Methods 6, 99-110.
17. Küttner, G., Gießmann, E., Niemann, B., Winkler, K., Grunow, R., Hinkula, J., Rosen, J., Wahren, B. and von Baehr, R. (1992) Immunoglobulin V regions and epitope mapping of a murine monoclonal antibody against p24 core protein of HIV-1. Mol. Immunol. 29, 561-564.
18. Mendes, P. (1993) GEPASI: a software package for modelling the dynamics, steady states and control of biochemical and other systems. Comput. Appl. Biosci. 9, 563-571.
19. Ong, G.L. and Mattes, M.J. (1993) Re-evaluation of the concept of functional affinity as applied to bivalent antibody binding to cell surface antigens. Mol. Immunol. 30, 1455-1462.
20. Roggenbuck, D., Marx, U., Kiessig, S.T., Schoenherr, G., Jahn, S. and Porstmann, T. (1994) Purification and immunochemical characterization of a natural human polyreactive monoclonal IgM antibody. J. Immunol. Methods 167, 207-218.
21. Rosé, S., Hensley, P., O'Shannessy, D.J., Culp, J., Debouck, C. and Chaiken, I. (1992) Characterization of HIV-1 p24 self-assoziation using analytical affinity chromatography. Proteins 13, 112-119.
22. Sjölander, S. and Urbaniczky, C. (1991) Integrated fluid handling system for bimolecular interaction analysis. Anal. Chem. 63, 2338-2345.