Detection of transforming growth factor-α mRNA and protein in rat lacrimal glands and characterization of transforming growth factor-α in human tears

Investigative Ophthalmology and Visual Science

Volumn 37, Issue 1, 1996, Pages 166-173

  Van Setten, G B ^a   Macauley, Shawn ^b   Humphreys Beher, Michael ^b   Chegini, Nasser ^b   Schultz, Gregory ^b,c  

^a KAROLINSKA INSTITUTE   (Sweden)

^b UNIVERSITY OF FLORIDA   (United States)

^c UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

Author keywords

epidermal growth factor (EGF); lacrimal gland; tear fluid; transforming growth factor (TGF ); wound healing

Indexed keywords

EPIDERMAL GROWTH FACTOR; MESSENGER RNA; TRANSFORMING GROWTH FACTOR ALPHA;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CORNEA INJURY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; LACRIMAL FLUID; LACRIMAL GLAND; LACRIMATION; MALE; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WOUND HEALING;

ADULT; ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; BLOTTING, WESTERN; DNA PRIMERS; EYE PROTEINS; FEMALE; HUMANS; IMMUNOENZYME TECHNIQUES; LACRIMAL APPARATUS; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; POLYMERASE CHAIN REACTION; RADIOIMMUNOASSAY; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; TEARS; TRANSCRIPTION, GENETIC; TRANSFORMING GROWTH FACTOR ALPHA;

EID: 0030053158     PISSN: 01460404     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. Maurice D. The Charles Prentice Award Lecture 1989: The physiology of tears. Optom Vis Sci. 1990;67:391-399.
2. van Setten GB, Tervo T, Tarkkanen A, Pesonen K, Viinikka L, Perheentupa J. Epidermal growth factor is a constant component of human tear fluid. Graefe's Arch Clin Exp Ophthalmol. 1989;227:84-87.
3. Ohashi Y, Motokura M, Kinoshita Y, et al. Presence of epidermal growth factor in human tears. Invest Ophthalmol Vis Sci. 1989;30:1879-1882.
4. Schultz G, Chegini N, Grant M, Khaw P, MacKay S. Effects of growth factors on corneal wound healing. Acta Ophthalmol (Copenh). 1992(suppl);202:60-66.
5. Schultz G, Khaw PT, Oxford K, Macauley S, van Setten G, Chegini N. Growth factors and ocular wound healing. Eye. 1994;8:184-187.
6. Woost PG, Brightwell J, Eiferman R, Schultz GS. Effect of growth factors with dexamethasone on healing of rabbit corneal stromal incisions. Exp Eye Res. 1985;40:47-60.
7. Schultz G, Cipolla L, Whitehouse A, Eiferman R, Woost P, Jumblatt M. Growth factors and corneal endothelial cells: III: Stimulation of adult human endothelial cell mitosis in vitro by defined mitogenic agents. Cornea. 1992;11:20-27.
8. van Setten GB, Schultz GS, Macauley S. Growth factors in human tear fluid and in lacrimal glands. In: Sullivan DA, ed. Lacrimal Gland, Tear Film, and Dry Eye Syndromes. New York: Plenem Press; Adv in Exp Med in Biol. 1994;350:315-320.
9. van Setten G, Schultz G. Transforming growth factor alpha is a constant component of human tear fluid. Graefe's Arch Clin Exp Ophthalmol. 1994;232:523-526.
10. Derynck R. Transforming growth factor-α. Cell. 1988;54:593-595.
11. Massagué J. Transforming growth factor-α. J Biol Chem. 1990;265:21393-21396.
12. Schreiber AB, Winkler ME, Derynck R. Transforming growth factor-α: A more potent angiogenic mediator than epidermal growth factor. Science. 1986;232:1250-1253.
13. Schultz GS, White M, Mitchell R, et al. Epithelial wound healing enchanced by transforming growth factor-α and vaccinia growth factor. Science. 1987; 235:350-352.
14. Ibbotson KJ, Harrod J, Gowen M, et al. Human recombinant transforming growth factor a stimulates bone resorption and inhibits formation in vitro. Proc Natl Acad Sci USA. 1986;83:2228-2232.
15. Barrando Y, Green H. Cell migration is essential for sustained growth of keratinocyte colonies: The roles of transforming growth factor-α and epidermal growth factor. Cell 1987;50:1131-1137.
16. Siegfried JM. Detection of human lung epithelial cell growth factors produced by a lung carcinoma cell line: Use in culture of primary solid lung tumors. Cancer Res. 1987;47:2903-2910.
17. Hollenberg MD, Muramatsu I, Ioth H, Patel P, Yang S-G, Lederis K. Contractile actions of epidermal growth factor-urogastrone in isolated smooth muscle preparations from guinea pig stomach: Structure-activity relationships and comparison with the effects of transforming growth factor-alpha. J Pharmacol Exp Ther. 1989;248:384-390.
18. Khaw PT, Schultz GS, MacKay SL, et al. Detection of transforming growth factor-alpha messenger RNA and protein in human corneal epithelial cells. Invest Ophthalmol Vis Sci. 1992;33:3302-3306.
19. van Setten GB, Tervo K, Virtanen I, Tarkkanen A, Tervo T. Immunohistochemical demonstration of epidermal growth factor in the lacrimal and submandibular glands of rats. Acta Ophthalmol (Copenh). 1990;68:477-480.
20. Humphreys-Beher MG, Macauley S, Chegini N, et al. Characterization of the synthesis and secretion of TGF-α from salivary glands and saliva. Endocrinology. 1994;134:963-970.
21. Chomczynski P, Sacchi N. Single step method of RNA isolation by guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162:156-159.
22. MacKay SLD, Yaswen L, Tarnuzzer RW, et al. Colon cancer cells that are not growth inhibited by TGF-β lack functional type I and type II TGF-β receptors. Ann Surg. 1995;221:767-777.
23. Derynck R, Roberts AB, Eaton DH, Winkler ME, Goeddel DV. Human transforming growth factor-α: Precursor sequence, gene structure, and heterologous expression. In: Feramisco J, Ozanne B, Stiles C, eds. Cancer Cells 3/Growth Factors and Transformation. Cold Spring Harbor: Cold Spring Harbor Laboratory; 1985:79-86.
24. Todaro GJ, Lee DC, Webb NR, Rose TM, Brown JP. Rat type-α transforming growth factor: structure and possible function as a membrane receptor. In: Feramisco J, Ozanne B, Stiles C, eds. Cancer Cells 3/Growth Factors and Transformation. Cold Spring Harbor: Cold Spring Harbor Laboratory; 1985:51-58.
25. van Setten GB. Epidermal growth factor in human tear fluid: Increased release but decreased concentrations during reflex tearing. Curr Eye Res. 1990;9:79-83.
26. Chard T. An introduction to radioimmunoassay and related techniques. In: Burdon RH, van Knippenberg PH, eds. Laboratory Techniques in Biochemistry and Molecular Biology. Amsterdam: Elsevier; 1990;6:17-22.
27. Katoh M, Inagaki H, Kurosawa-Ohsawa K, Katsuura M, Tanaka S. Detection of transforming growth factor alpha in human urine and plasma. Biochem Biophys Res Comm. 1990;167:1065-1072.
28. Grant MB, Khaw PT, Schultz GS, Adams JL, Shimizu RW. Effects of epidermal growth factor, fibroblast growth factor, and transforming growth factor-β on corneal cell chemotaxis. Invest Ophthalmol Vis Sci. 1992;33:3292-3301.
29. Okada M, Ohmura E, Kamiya Y, et al. Transforming growth factor (TGF)-alpha in human milk. Life Sci. 1991;48:1151-1156.
30. 125I-labeled transforming growth factor-α from epidermal growth factor in human urine. Cancer Res. 1986;46:6004-6010.
31. Winkler ME, O'Conner L, Winget M, Fendly B. Epidermal growth factor and transforming growth factor α bind differently to the epidermal growth factor receptor. Biochemistry. 1989;28:6373-6378.
32. Mead JE, Fausto N. Transforming growth factor α may be a physiological regulator of liver regeneration by means of an autocrine mechanism. Proc Natl Acad Sci USA. 1989;86:1558-1562.