Cloning of mouse DAN cDNA and its down-regulation in transformed cells

Japanese Journal of Cancer Research

Volumn 87, Issue 1, 1996, Pages 58-61

  Ozaki, Toshinori ^a   Ma, Jim ^a   Takenaga, Keizo ^a   Sakiyama, Shigeru ^a  

^a CHIBA CANCER CENTER   (Japan)

Author keywords

DAN gene; Down regulation; NIH3T3 cell line

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; GENE PRODUCT;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CANCER INHIBITION; CELL TRANSFORMATION; CODING; DNA SEQUENCE; FIBROBLAST; GENE EXPRESSION; HUMAN; HUMAN CELL; LUNG; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; RAT; SEQUENCE ANALYSIS; SOUTHERN BLOTTING;

EID: 0030051697     PISSN: 09105050     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1349-7006.1996.tb00200.x     Document Type: Article

References (15)

1. Pachnis, V., Belayew, A. and Tilghman, S. M. Locus unlinked to α-fetoprotein under the control of the murine raf and Rif genes. Proc. Natl. Acad. Sci. USA, 81, 5523-5527 (1984).
2. Steeg, P. S., Bevilacqua, G., Kopper, L., Thorgeirsson, U. P., Talmadge, J. E., Liotta, L. A. and Sobel, M. E. Evidence for a novel gene associated with low tumor metastatic potential. J. Natl. Cancer Inst., 80, 200-204 (1988).
3. Contente, S., Kenyon, K., Rimoldi, D. and Friedman, R. M. Expression of gene rrg is associated with reversion of NIH3T3 transformed by LTR-c-H-ras. Science, 249, 796-798 (1990).
4. Dowdy, S. F., Lai, K.-M., Weissman, B. E., Matsui, Y., Hogan, B. L. M. and Stanbridge, E. J. The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells. Nucleic Acids Res., 19, 5763-5769 (1991).
5. El-Deiry, W. S., Tokino, T., Velculescu, V. E., Levy, D. B., Parsons, R., Trent, J. M., Lin, D., Mercer, W. E., Kinzler, K. W. and Vogelstein, B. WAF1, a potential mediator of p53 tumor suppression. Cell, 75, 817-825 (1993).
6. Zou, Z., Anisowicz, A., Hendrix, M. J. C., Thor, A., Neveu, M., Sheng, S., Rafidi, K., Seftor, E. and Sager, R. Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells. Science, 263, 526-529 (1994).
7. Lee, S. W., Tomasetto, C. and Sager, R. Positive selection of candidate tumor-suppressor genes by subtractive hybridization. Proc. Natl. Acad. Sci. USA, 88, 2825-2829 (1991).
8. Ozaki, T. and Sakiyama, S. Molecular cloning and characterization of a cDNA showing negative regulation in v-src-transformed 3Y1 rat fibroblasts. Proc. Natl. Acad. Sci. USA, 90, 2593-2597 (1993).
9. Ozaki, T. and Sakiyama, S. Tumor-suppressive activity of N03 gene product in v-src-transformed rat 3Y1 fibroblasts. Cancer Res., 54, 646-648 (1994).
10. Enomoto, H., Ozaki, T., Takahashi, E., Nomura, N., Tabata, S., Takahashi, H., Ohnuma, N., Tanabe, M., Iwai, J., Yoshida, H., Matsunaga, T. and Sakiyama, S. Identification of human DAN gene, mapping to the putative neuroblastoma tumor suppressor locus. Oncogene, 9, 2785-2791 (1994).
11. Feinberg, A. P. and Vogelstein, B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem., 132, 6-13 (1984).
12. Denhardt, D. A membrane filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun., 23, 640-646 (1966).
13. Sambrook, J., Fritsch, E. F. and Maniatis, T. "Molecular Cloning: A Laboratory Manual" (1989). Cold Spring Harbor Laboratory Press, New York.
14. Sanger, F., Nicklen, S. and Coulson, A. R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA, 74, 5463-5467 (1977).
15. Ozaki, T., Nakamura, Y., Enomoto, H., Hirose, M. and Sakiyama, S. Overexpression of DAN gene product in normal rat fibroblasts causes a retardation of the entry into the S phase. Cancer Res., 55, 895-900 (1995)