The embryo-specific EMB-1 protein of Daucus carota is flexible and unstructured in solution

Plant Science

Volumn 115, Issue 1, 1996, Pages 17-24

  Eom, Juwhan ^a   Baker, Wayne R ^a   Kintanar, Agustin ^a   Wurtele, Eve Syrkin ^a  

^a IOWA STATE UNIVERSITY   (United States)

Author keywords

Daucus carota; Embryo; NMR; Seed desiccation; Three dimensional structure

Indexed keywords

DAUCUS CAROTA;

EID: 0030050554     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-9452(96)04332-4     Document Type: Article

References (18)

1. L. Dure, III., M. Crouch, J. Harada, T.-H.D. Ho, J. Mundy, R. Quatrano, T. Thomas and Z.R. Sung, Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol. Biol., 12 (1989) 475-486.
2. A.C. Cuming, Developmental regulation of gene expression in wheat embryos. Molecular cloning of a DNA sequence encoding the early-methionine-labeled polypeptide. Eur. J. Biochem., 145 (1984) 351-357.
3. M. Espelund, S. Saeboe-Larssen, D.W. Hughs, G.A. Galau, F. Larsen and K.S. Jakobsen, Late embryogenesis-abundant genes encoding proteins with different numbers of hydrophilic repeats are regulated differentially by abscisic acid and osmotic stress. Plant J., 2 (1992) 241-252.
4. E.S. Wurtele, H. Wang, S. Durgerian, B.J. Nikolau and T.H. Ulrich, Characterization of a gene that is expressed early in somatic embryogenesis of Daucus carota. Plant Physiol., 102 (1993) 303-312.
5. T.U. Ulrich, E.S. Wurtele and B.J. Nikolau, Sequence of EMB-1, an mRNA accumulating specifically in embryos of carrot. Nucl. Acids Res., 18 (1990) 2826.
6. T. Hattori, T. Terada, and S. Hamasuna, Regulation of the Osem gene by abscisic acid and the transcriptional activator VP1: analysis of cis-acting promoter elements required for regulation by abscisic acid and VP1. Plant J., 7 (1995) 913-925.
7. R.R. Finklestein, Abscisic acid-insensitive mutations provide evidence for stage specific signal pathways regulating expression of an Arabidopsis late embryogenesis abundant (lea) gene. Mol. Gen. Genet., 238 (1993) 401-408.
8. W.D. McCubbin, C.M. Cay and B.C. Lane, Hydrodynamic and optical properties of the wheat germ Em protein. Can. J. Biochem. Cell Biol., 63 (1985) 803-811.
9. F.W. Studier, A.H. Rosenberg, J.J. Dunn and J.W. Dubendorff, Use of the T7 RNA polymerase to direct expression of cloned genes. Meth. Enzymol., 185 (1990) 60-89.
10. J. Sambrook, E.F. Fritsch and T. Maniatis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
11. F.W. Studier and B.A. Moffatt, Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol., 189 (1986) 113-130.
12. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 227 (1970) 680-685.
13. X. Wang, E.S. Wurtele, G. Keller, A.L.McKean and B.J. Nikolau, Molecular cloning of cDNAs and genes coding for beta-methylcrotonyl-CoA carboxylase of tomato. J Biol Chem., 269 (1994) 11760-11769.
14. J. Jeener, B.H. Meier, P. Bachmann and R.R. Errnst, Investigation of exchange processes by two-dimensional NMR spectroscopy. J. Chem. Phys., 71 (1979) 4546-4553.
15. D.J. States, R.A. Haberkorn, and D.J. Ruben, A two-dimensional nuclear Overhauser experiment with pure absorption phase in four quadrants. J. Magn. Reson., 48 (1982) 286-292.
16. K. Wuthrich, NMR of Proteins and Nucleic Acids, John Wiley and Sons, New York, 1986.
17. I.D. Campbell and R.A. Dwek, Biological Spectroscopy, Benjamin/Cummings, Menlo Park, CA, 1984.
18. J.C. Litts, G.W. Colwell, R.L. Chakerian and R.S. Quatrano, The nucleotide sequence of a cDNA clone encoding the wheat Em protein. Nucl. Acids Res., 15 (1987) 3607-3618.