Electrophoretic forms of chitinolytic and lysozyme activities in ruminal protozoa

Current Microbiology

Volumn 32, Issue 3, 1996, Pages 115-118

  Morgavi, Diego P ^a   Sakurada, Masaru ^a   Tomita, Yoshifumi ^a   Onodera, Ryoji ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHITINASE; DITHIOTHREITOL; DODECYL SULFATE SODIUM; LYSOZYME; N ACETYL BETA GLUCOSAMINIDASE;

ARTICLE; ENZYME DEGRADATION; ENZYME DENATURATION; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTOZOON;

ACETYLGLUCOSAMINIDASE; ANIMALS; CHITINASE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GOATS; MURAMIDASE; PROTOZOA;

ENTODINIUM CAUDATUM; GALLUS GALLUS; PROTOZOA;

EID: 0030050330     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002849900020     Document Type: Article

References (19)

1. Audy P, Trudel J, Asselin A (1988) Purification and characterization of a lysozyme from wheat germ. Plant Sci 58:43-50
2. Audy P, Grenier J, Asselin A (1989) Lysozyme activity in animal extracts after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Comp Biochem Physiol 92B:523-527
3. Coleman GS, Laurie JI (1974) The utilization of Bacillus megaterium and the release of a lytic enzyme by three Epidinium spp. isolated from the rumen. J Gen Microbiol 85:257-264
4. Eadie JM (1962) The development of rumen microbial populations in lambs and calves under various conditions of management. J Gen Microbiol 29:563-578
5. Ghuysen J-M, Tipper DJ, Strominger JL (1966) Enzymes that degrade bacterial cell walls. Methods Enzymol 8:685-699
6. Gooday GW (1990a) Chitinases. In: Leatham G, Himmel M (eds) Enzymes in biomass conversion, Chapter 37. Washington: American Chemical Society Books, pp 478-485
7. Gooday GW (1990b) Physiology of microbial degradation of chitin and chitosan. Biodegradation 1:177-190
8. Jeuniaux C (1966) Chitinases. Methods Enzymol 8:645-650
9. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
10. Ling JR (1990) Digestion of bacterial cell walls in the rumen. In: Hoshino S, Onodera R, Minato H, Itabashi H (eds) The rumen ecosystem. The microbial metabolism and its regulation, Tokyo: Japan Scientific Societies Press, pp 83-90
11. Majeau N, Trudel J, Asselin A (1990) Diversity of cucumber chitinase isoforms and characterization of one seed basic chitinase with lysozyme activity. Plant Sci 68:9-16
12. McGrew BR, Green DM (1990) Enhanced removal of detergent and recovery of enzymatic activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of casein in gel wash buffer. Anal Biochem 189:68-74
13. Molano J, Polacheck I, Duran A, Cabib E (1979) An endochitinase from wheat germ: activity on nascent and preformed chitin. J Biol Chem 254:4901-4907
14. Morgavi DP, Sakurada M, Tomita Y, Onodera R (1994a) Multiple forms of chitinase in a mixed rumen protozoa population and in Entodinium caudatum. In: Shimada K, Hoshino S, Ohmiya K, Sakka K, Kobayashi Y, Karita S (eds) Genetics, biochemistry and ecology of lignocellulose degradation. Toba, Mie Prefecture, Japan: Uni Publishers Co., Ltd., pp 208-215
15. Morgavi DP, Sakurada M, Tomita Y, Onodera R (1994b) Presence in rumen bacterial and protozoal populations of enzymes capable of degrading fungal cell walls. Microbiology 140:631-636
16. Onodera R, Henderson C (1980) Growth factors of bacterial origin for the culture of the rumen oligotrich protozoon, Entodinium caudatum. J Appl Bacteriol 48:125-134
17. Onodera R, Yamaguchi H, Eguchi C, Kandatsu M (1977) Limits of survival of the mingled rumen bacteria in the washed cell suspension of rumen ciliate protozoa. Agric Biol Chem 41:2465-2466
18. Teunissen MJ, deKort GVM, OpdenCamp HJM, Huisin'tVeld JHJ (1992) Production of cellulolytic and xylanolytic enzymes during growth of the anaerobic fungus Piromyces sp. on different substrates. J Gen Microbiol 138:1657-1664
19. Trudel J, Asselin A (1989) Detection of chitinase after polyacrylamide gel electrophoresis. Anal Biochem 178:362-366