Differentiation of Listeria isolates by PCR amplicon profiling and sequence analysis of 16S-23S rRNA internal transcribed spacer loci

Journal of Applied Bacteriology

Volumn 80, Issue 2, 1996, Pages 174-178

  Drebot M ^a   Neal, S ^b   Schlech, W ^b   Rozee, K ^a  

^a Department of Microbiology and 'Immunology ^*  (Canada)

^b QUEEN ELIZABETH II HEALTH SCIENCES CENTRE   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

LISTERIA; POLYMERASE CHAIN REACTION; RNA; STRAIN; TRANSCRIPTION;

AGAROSE GEL; AMPLICONS; DIRECT SEQUENCING; GENOMIC DNA; INTERNAL TRANSCRIBED SPACERS; L.MONOCYTOGENES; LISTERIA SPECIES; SEQUENCE ANALYSIS; SPECIES DIFFERENTIATIONS; STRAIN DIFFERENTIATION;

DISEASES;

RNA 16S;

ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CONTROLLED STUDY; DNA SEQUENCE; LISTERIA; NONHUMAN;

BACTERIA (MICROORGANISMS); LISTERIA; LISTERIA MONOCYTOGENES;

EID: 0030048475     PISSN: 00218847     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2672.1996.tb03206.x     Document Type: Article

References (13)

1. Barry, T., Colleran, G., Glennon, M., Dunican, L.K. and Gannon, F. (1991) The 16S/23S ribosomal spacer region as a target for DNA probes to identify eubacteria. PC R Methods Applications 1, 51-56.
2. Bram, R.J., Young, R.A. and Steitz, J.A. (1980) The ribonuclease III site flanking 23S sequences in the 30S ribosomal precursor RNA of E. colt. Cell 19, 393-401.
3. Czajka, J., Bsat, N., Piani, M., Russ, W., Sultana, K., Wiedmann, M., Whitaker, R. and Batt, C.A., (1993) Differentiation ofListena monocytogenes and Listeria innocua by 16S rRNA genes and intraspecies discrimination of Listeria monocytogenes strains by random amplified polymorphic DNA polymorphisms. Applied and Environmental Microbiology 59, 304-308.
4. Drebôt, M.A. and Lee, S.H.S. (1994) Direct sequencing of doublestranded PCR products gel purified through blotting paper. Bio Techniques 17, 6-7.
5. Emond, E., Fliss, I. and Pandian, S. (1993) A ribosomal DNA fragment of Listeria monocytogenes and its use as a genus-specific probe in an aqueous-phase hybridization assay. Applied and Environmental Microbiology 59, 2690-2697.
6. Jensen, M.A., Webster, J.A. and Straus, N. (1993) Rapid identification of bacteria on the basis of polymerase chain reactionamplified ribosomal DNA spacer polymorphisms. Applied and Environmental Microbiology 59, 945-952.
7. Lane, D.J., Pace, B., Olsen, G.L., Stahl, D.A., Sogin, M.L. and Pace, N.R. (1985) Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proceedings of the National Academy of Sciences USA 82, 6955-6959.
8. Lehner, A.F., Harvey, S. and Hill, C.W. (1984) Mapping and spacer identification of rRNA opérons of Salmonella typhimurium. Journal of Bacteriology 160, 682-686.
9. Loughney, K., Lund, E. and Dahlberg, J.E. (1982) tRNA genes were found between the 16S and 23S genes in Bacillus subti/is. Nucleic Acids Research 10, 1607-1624.
10. Lund, E. and Dahlberg, J.E. (1977) Spacer transfer RNAs in transcripts of E. coli. Processing of 30S ribosomal RNA in vitro. Cell 11,247-262.
11. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences USA 74, 5463-5467.
12. Weisburg, W.G., Barns, S.M., Pelletier, D.A. and Lane, DJ. (1991) 16S ribosomal DNA amplificaiton for phylogenetic study. Journal of Bacteriology 173, 697-703.
13. Young, R.A. and Steitz, J.A. (1978) Complementary sequences 1700 nucleotides apart from a ribonuclease III cleavage site in Eschenchia coli ribosomal precursor RNA. Proceedings of the National Academy of Sciences USA 75, 3593-3597.