The fluorescent probe HPTS as a phloem-mobile, symplastic tracer: An evaluation using confocal laser scanning microscopy

Journal of Experimental Botany

Volumn 47, Issue 296, 1996, Pages 439-445

  Wright, Kathryn M ^a   Oparka, Karl J ^a  

^a SCOTTISH CROP RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Confocal laser scanning microscopy (CLSM); HPTS; Intercellular transport; Phloem (unloading); Symplast

Indexed keywords

ABUTILON SPP.; ARABIDOPSIS SPP.;

EID: 0030047996     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jxb/47.3.439     Document Type: Review

References (27)

1. Cole L, Coleman J, Kearns A, Morgan G, Hawes C. 1991. The organic anion transport inhibitor, Probenecid. inhibits the transport of Lucifer Yellow at the plasma membrane and the tonoplast in suspension-cultured plant cells. Journal of Cell Science 99, 545-55.
2. Cook H, Oparka KJ. 1983. Movement of fluorescein into isolated caryopses of wheat and barley. Plant, Cell and Environment 6, 239-42.
3. Duckett CM, Oparka KJ, Prior DAM, Dolan L, Roberts K. 1994. Dye-coupling in the root epidermis of Arabidopsis is progressively reduced during development. Development 120, 3247-55.
4. Findlay N, Mercer FV. 1971a. Nectar production in Abutilon. I. Movement of nectar through the cuticle. Australian Journal of Biological Sciences 24, 647-56.
5. Findlay N, Mercer FV. 1971b. Nectar production in Abutilon. II. Submicroscopic structure of the nectary. Australian Journal of Biological Sciences 24, 657-64.
6. Grignon N, Touraine B, Durand M. 1989. 6(5) Carboxyfluorescein as a tracer of phloem sap translocation. American Journal of Botany 76, 871-7.
7. Gunning BES, Hughes JE. 1976. Quantitative assessment of symplastic transport of pre-nectar into the trichomes of Abutilon nectaries. Australian Journal of Plant Physiology 3, 619-37.
8. Lucas WJ, Ding B, van der Schoot C. 1993. Plasmodesmata and the supracellular nature of plants. New Phytologist 125, 435-76.
9. Lucas WJ, Wolf S. 1993. Plasmodesmata: the intercellular organelles of green plants. Trends in Cell Biology 3, 308-15.
10. Oparka KJ. 1991. Uptake and compartmentation of fluorescent probes by plant cells. Journal of Experimental Botany 42, 565-79.
11. Oparka KJ. 1993. Signalling via plasmodesmata - the neglected pathway. Seminars in Cell Biology 4, 131-8.
12. Oparka KJ, Duckett CM, Prior DAM, Fisher DB. 1994. Real-time imaging of phloem unloading in the root tip of Arabidopsis. The Plant Journal 6, 759-66.
13. Palevitz BA, Hepler PK. 1985. Changes in dye coupling of stomatal cells of Allium and Commelina demonstrated by microinjection of Lucifer Yellow. Planta 164, 473-9.
14. Peterson CA, Emanuel ME, Humphreys GB. 1981. Pathway of movement of apoplastic fluorescent dye tracers through the endodermis at the site of secondary root formation in com (Zea mays) and broad bean (Vicia faba). Canadian Journal of Botany 59, 618-25.
15. Robards AW, Lucas WJ. 1990. Plasmodesmata. Annual Review of Plant Physiology and Plant Molecular Biology 41, 369-419.
16. Robards AW, Stark M. 1988. Nectar secretion in Abutilon: a new model. Protoplasma 142, 79-91.
17. Schumacher W. 1933. Untersuchungen uber die Wanderung des fluoresceins in den Siebrohren. Jahrbuch für Wissenschaftliche Botanik 77, 685-732.
18. Socolar SJ, Loewenstein WR. 1979. Cell-to-cell junctional transmission. In: Korn ED, ed. Methods in membrane biology 10. New York: Plenum Press. 123-79.
19. Stewart WW. 1981. Lucifer dyes - highly fluorescent dyes for biological tracing. Nature 292, 17-21.
20. Terry BR, Robards AW. 1987. Hydrodynamic radius alone governs the mobility of molecules through plasmodesmata. Planta 171, 145-57.
21. Tucker JE, Mauzerall D, Tucker EB. 1989. Symplastic transport of carboxyfiuorescein in staminal hairs of Setcreasea purpurea is diffusive and includes loss to the vacuole. Plant Physiology 90, 1143-7.
22. van Bel AJE, Kempers R. 1990. Symplastic isolation of the sieve element-companion cell complex in the phloem of Ricinus communis and Salix alba stems. Planta 183, 69-76.
23. Wang HL, Offler CE, Patrick JW, Ugalde TD. 1994. The cellular pathway of photosynthate transfer in the developing wheat grain. I. Delineation of a potential transfer pathway using fluorescent dyes. Plant, Cell and Environment 17, 257-66.
24. Wang N, Fisher DB. 1994. The use of fluorescent tracers to characterize the post-phloem transport pathway in maternal tissues of developing wheat grains. Plant Physiology 104, 17-27.
25. Wright KM, Oparka KJ. 1994. Physicochemical properties alone do not predict the movement and compartmentation of fluorescent xenobiotics. Journal of Experimental Botany 45, 35-44.
26. Zellnig G, Kronestedt-Robards EC, Robards AW. 1991. Intercellular permeability in Abutilon nectary trichomes. Protoplasma 161, 150-9.
27. Ziegler H. 1975. Nature of transported substances. In: Zimmermann MH, Milburn J. eds. Encyclopedia of plant physiology. New edn. Vol. I. Phloem transport. Berlin: Springer-Verlag, 59-100.