The identification of transposon-tagged mutations in essential genes that affect cell morphology in Saccharomyces cerevisiae

Genetics

Volumn 142, Issue 1, 1996, Pages 39-50

  Chun, Kristin T ^a   Goebl, Mark G ^a  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL STRUCTURE; FUNGUS GROWTH; FUNGUS MUTATION; GENE MUTATION; GENE SEQUENCE; GROWTH REGULATION; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; TRANSPOSON;

AMINO ACID SEQUENCE; BASE SEQUENCE; CHROMOSOME MAPPING; DNA PRIMERS; DNA TRANSPOSABLE ELEMENTS; DNA, FUNGAL; FUNGAL PROTEINS; GENES, FUNGAL; GENES, LETHAL; GENOMIC LIBRARY; HAPLOIDY; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; OPEN READING FRAMES; PHENOTYPE; SACCHAROMYCES CEREVISIAE; SEQUENCE TAGGED SITES; SPORES, FUNGAL;

FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0030045536     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (48)

1. ADAMS, A. E., 1985 Cellular morphogenesis in the yeast Saccharomyces cerevisiae. Ph.D. dissertation, University of Michigan, Ann Arbor.
2. ADAMS, M. D., J. M. KELLEY, J. D. GOCAYNE, M. DUBNICK, M. H. POLYMEROPOULOS et al., 1991 Complementary DNA sequencing: expressed sequence tags and human genome project. Science 252: 1651-1656.
3. ADAMS, M. D., A. R. KERLAVAGE, C. FIELDS and J. C. VENTER, 1993 3,400 new expressed sequence tags identify diversity of transcripts in human brain. Nat. Genet. 4: 256-267.
4. ALTSCHUL, S. F., W. GISH, W. MILLER, E. W. MYERS and D. J. LIPMAN, 1990 Basic local alignment search tool. J. Mol. Biol. 215: 403-410.
5. BARTEL, B., and A. VARSHAVSKY, 1988 Hypersensitivity to heavy water: a new conditional phenotype. Cell 52: 935-941.
6. BOTSTEIN, D., S. C FALCO, S. E STEWART, M. BRENNAN, S. SCHERER et al., 1979 Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24.
7. BRILL, S. J., and B. STILLMAN, 1991 Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase. Genes Dev. 5: 1589-1600.
8. BROACH, J. R., J. N. STRATHERN and J. B. HICKS, 1979 Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133.
9. BURNS, N., B. GRIMWADE, P. B. ROSS-MACDONALD, E.-Y. CHOI, K. FINBERG et al., 1994 Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. Genes Dev. 8: 1087-1105.
10. BYERS, B., 1981 Multiple roles of the spindle pole bodies in the life cycle of Saccharomyces cerevisiae, pp. 119-133 in Molecular Genetics in Yeast, Alfred Benzon Symposium, edited by D. VON WETTSTEIN, J. FRIIS, M. KIELLAND-BRANDT, and A. STENDERUP. Munksgaard, Copenhagen.
11. BYERS, B., and L. GOETSCH, 1974 Duplication of spindle plaques and integration of the yeast cell cycle. Cold Spring Harbor Symp. Quant. Biol. 38: 123-131.
12. DAVIDOW, L. S., and B. BYERS, 1984 Enhanced gene conversion and postmeiotic segregation in pachytene-arrested Saccharomyces cerevisiae. Genetics 106: 165-183.
13. DAVIS, R. W , D. BOTSTEIN and J. R. ROTH, 1980 A Manual for Genetic Engineering: Advanced Bacterial Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
14. DOBSON, M. J., A. B. FUTCHER and B. S COX, 1980 Loss of 2 μm DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219. Curr. Genet. 2: 201-205.
15. FORD, S. K., and J. R. PRINGLE, 1991 Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC11 gene product and the timing of events at the budding site. Dev. Genet. 12: 281-292.
16. GERRING, S. L., C. CONNELLY and P. HIETER, 1991 Positional mapping of genes by chromosome blotting and chromosome fragmentation. Methods Enzymol. 194: 57-77.
17. GOEBL, M. G., and T. D. PETES, 1986 Most of the yeast genomic sequences are not essential for cell growth and division. Cell 46: 983-992.
18. GOEBL, M. G., J. YOCHEM, S. JENTSCH, J. P. MCGRATH, A. VARSHAVSKY et al., 1988 The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme. Science 241: 1331-1335.
19. GOFFEAU, A., 1994 Genes in search of functions Nature 369: 101-102.
20. HAARER, B. K., and J. R PRINGLE, 1987 Immunofluorescence localization of the Saccharomyces cerevisiae CDC12 gene product to the vicinity of the 10-nm filaments in the mother-bud neck. Mol. Cell. Biol. 7: 3678-3687.
21. HARRIS, S. D., J. CHENG, T. A. PUGH and J. R. PRINGLE, 1992 Molecular analysis of Saccharomyces cerevisiae chromosome I: on the number of genes and the identification of essential genes using temperature-sensitive-lethal mutations. J. Mol. Biol. 225: 53-65.
22. HARTWELL, L. H., 1967 Macromolecule synthesis in temperature-sensitive mutants of yeast. J. Bacteriol. 93: 1662-1670.
23. HOEKSTRA, M. F., H. S. SEIFERT, J. NICKOLOFF and F. HEFFRON, 1991 Shuttle mutagenesis: bacterial transposons for genetic manipulations in yeast. Methods Enzymol. 194: 329-342.
24. HOLLINGSWORTH, N. M., and B. BYERS, 1989 HOP1: a yeast meiotic pairing gene. Genetics 121: 445-462.
25. HUTTER, K. J., and H. E. EIPEL, 1979 Microbial determinations by flow cytometry. J. Gen. Microbiol. 113: 369-375.
26. JOHNSON, D. I., C. W. JACOBS, J. R. PRINGLE, L. C. ROBINSON, G F. CARLE et al., 1987 Mapping of the Saccharomyces cerevisiae CDC3, CDC25, and CDC42 genes to chromosome XII by chromosome blotting and tetrad analysis. Yeast 3: 243-253.
27. KABACK, D. B., P. W OELLER H Y STEENSMA, J. HIRSCHMAN, D. RUEZINSKY, et al., 1984 Temperature-sensitive lethal mutations on yeast chromosome I appear to define only a small number of genes. Genetics 108: 67-90.
28. KASSIR, Y., and G. SIMCHEN, 1991 Monitoring meiosis and sporulation in Saccharomyces cerevisiae. Methods Enzymol. 194: 94-110.
29. KAUFER, N. F., H. M. FRIED, W. F. SCHWINDINGER, M. JASIN and J. R. WARNER, 1983 Cycloheximide resistance in yeast: the gene and its protein. Nucleic Acids Res. 11: 3123-3135.
30. KIM, H. B., B. K. HAARER and J. R. PRINGLE, 1991 Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC3 gene product and the timing of events at the budding site. J. Cell Biol. 112: 535-544.
31. KLECKNER, N , 1981 Transposable elements in prokaryotes. Annu. Rev. Genet. 15: 341-404.
32. LEVY, J., 1994 Sequencing the yeast genome: an international achievement. Yeast 10: 1689-1706.
33. LEW, D. J., and S. I. REED, 1993 Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins. J. Cell Biol 120: 1305-1320.
34. MATHIAS, N., M. BAYÉS and C. TYLER-SMITH, 1994 Highly informative compound haplotypes for the human Y chromosome. Hum. Mol. Genet. 3: 115-123.
35. MOIR, D., S. E. STEWART, B. C. OSMOND and D. BOTSTEIN, 1982 Cold-sensitive cell-division-cycle mutants of yeast: isolation, properties, and pseudoreversion studies. Genetics 100: 547-563.
36. MOORE, E. D., 1987 Overview of recombinant DNA libraries, pp. 5.1.1-5.1.3 in Current Protocols in Molecular Biology, edited by F. M. AUSUBEL, R. BRENT, R. E. KINGSTON, D. D. MOORE, J. G. SEIDMAN, J. A. SMITH, and K. STRUHL. Greene Publishing Associates, Brooklyn.
37. OLSON, M. V., 1991 Genome structure and organization in Saccharomyces cerevisiae, pp. 1-39 in Molecular and Cellular Biology of the Yeast Saccharomyces: Genome Dynamics, Protein Synthesis, and Energetics, edited by J. R. BROACH, J. PRINGLE, and E. W. JONES. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
38. PHILIPPSEN, P., A. STOTZ and C. SCHERF, 1991 DNA of Saccharomyces cerevisiae. Methods Enzymol. 194: 169-182.
39. PRINGLE, J. R., and L. H. HARTWELL, 1981 The Saccharomyces cerevisiae cell cycle, 97-142 in The Molecular Biology of the Yeast Saccharomyces, Life Cycle and Inheritance, edited by J. N. STRATHERN, E. W. JONES, and J. R. BROACH Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
40. RILES, L., and M. V. OLSON, 1988 Nonsense mutations in essential genes of Saccharomyces cerevisiae. Genetics 118: 601-607.
41. ROSE, M. D., F. WINSTON and P. HIETER, 1990 Methods in Yeast Genetics: A Laboratory Course Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
42. SAMBROOK, J., E. F. FRITSCH and T. MANIATIS, 1989 Molecular Cloning: A Laboratory Manual, Ed. 2. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
43. SCHIESTL, R. H., and R. D. GIETZ, 1989 High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr. Genet. 16: 339-346.
44. SHERMAN, F., 1991 Getting started with yeast. Methods Enzymol. 194: 3-21.
45. SHERMAN, F., and J. HICKS, 1991 Micromanipulation and dissection of asci. Methods Enzymol. 194: 21-37.
46. SHERMAN, F., G. R. FINK and J. B. HICKS, 1986 Laboratory Course Manual for Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
47. SIKORSKI, R. S., and P. HIETER, 1989 A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27.
48. YAMAMOTO, A., D. B. DEWALD, I. V. BORONENKOV, R. A. ANDERSON, S. D. EMR et al., 1995 Novel PI(4) P 5-kinase homologue, Fablp, essential for normal vacuole function and morphology in yeast. Mol. Biol. Cell 6: 525-539.