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2. The retinas were placed (ganglion cell layer up) in a temperature-controlled chamber (31°to 34°C; Medical Systems) mounted on a stage ol either an inverted microscope (Nikon, Diaphot 300) or an upright microscope (Technical Instruments) and continuously perfused.
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note
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We thank D. Bentley for lending us a SIT camera, R. Yakura for assistance in the image processing, E. M. Messersmith for assistance on the histology, and R. Gallego and P. Dayan for useful discussions. Supported by NSF IBN 93-19539; March of Dimes; NIMH 48108 (C.J.S.); U. C. Berkeley Miller Fellowship (M.B.F.); NIH grants GM07048 (D.S.). EY00561 (F.S.W.), and EY06292 (D.P.W.); and of the Howard Hughes Medical Institute (C.J.S.).
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