Secretion of the mycobacterial 19-kilodalton protein by Escherichia coli, a novel method for the purification of recombinant mycobacterial antigens

Clinical and Diagnostic Laboratory Immunology

Volumn 3, Issue 1, 1996, Pages 23-29

  Hewinson, R G ^a   Harris, D P ^b   Whelan, A ^a   Russell, W P ^a  

^a VETERINARY LABORATORIES AGENCY   (United Kingdom)

^b HAMMERSMITH HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTATHIONE TRANSFERASE; MYCOBACTERIUM ANTIGEN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANTIGENICITY; ARTICLE; DNA SEQUENCE; ESCHERICHIA COLI; IMMUNIZATION; MOUSE; MYCOBACTERIUM BOVIS; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE;

EID: 0030042549     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/cdli.3.1.23-29.1996     Document Type: Article

References (20)

1. Ashbridge, K. R., B. T. Backstrom, H.-X. Liu, T. Vikerfors, D. R. Englebretsen, D. R. K. Harding, and J. D. Watson. 1992. Mapping of T helper cell epitopes by using peptides spanning the 19-kDa protein of Mycobacterium tuberculosis. J. Immunol. 148:2248-2255.
2. Ashbridge, K. R., R. J. Booth, J. D. Watson, and R. B. Lathigra. 1989. Nucleotide sequence of the 19 kDa antigen from Mycobacterium tuberculosis. Nucleic Acids Res. 17:1249.
3. Booth, R. J., D. L. Williams, K. D. Moudggil, L. C. Noonan, P. M. Grandison, J. J. McKee, R. L. Prestidge, and J. D. Watson. 1993. Homologs of Mycobacterium leprae 18-kilodalton and Mycobacterium tuberculosis 19-kilodalton antigens in other mycobacteria. Infect. Immun. 61:1509-1515.
4. Chaparas, S. D., C. J. Moloney, and S. R. Hendrik. 1970. Specificity of tuberculins and antigens from various species of mycobacteria. Am. Rev. Respir. Dis. 101:74-83.
5. Collins, M., A. Paiki, S. Wall, A. Nolan, J. Goodger, M. J. Woodward, and J. Dale. 1990. Cloning and characterization of the gene for the 19 kDa antigen of Mycobacterium bovis. J. Gen. Microbiol. 136:1429-1436.
6. Daniel, T. M. 1984. Soluble mycobacterial antigens, p. 417-465. In G. P. Kubica and L. G. Lawrence (ed.), The mycobacteria: a source book. Microbiology series vol. 15. Marcel Dekker, Inc., New York.
7. Deres, K., H. Schild, K.-H. Wiesmüller, G. Jung, and H.-G. Rammensee. 1989. In vivo priming of virus-specific cytotoxic T lymphocytes with synthetic lipopeptide vaccine. Nature (London) 342:561-564.
8. Fifis, T., C. Costopoulos, L. A. Corner, and P. R. Wood. 1992. Serological reactivity to Mycobacterium bovis protein antigens in cattle. Vet. Microbiol. 30:343-353.
9. Fifis, T., C. Coslopoulos, A. J. Radford, A. Bacic, and P. R. Wood. 1991. Purification and characterization of major antigens from a Mycobacterium bovis culture filtrate. Infect. Immun. 59:800-807.
10. Fifis, T., P. Plackett, L. A. Corner, and P. R. Wood. 1989. Purification of a major Mycobacterium bovis antigen for the diagnosis of bovine tuberculosis. Scand. J. Immunol. 29:91-101.
11. Garbe, T., D. P. Harris, H. M. Vordermeier, R. Lathigra, J. Ivanyi, and D. B. Young. 1993. Expression of the Mycobacterium tuberculosis 19-kilodalton antigen in Mycobacterium smegmatis: immunological analysis and evidence of glycosylation. Infect. Immun. 61:260-267.
12. Green, H. H. 1946. Weybridge PPD tuberculins. Br. Vet. J. 102:267-278.
13. Griffin, J. F. T., S. Nagai, and G. S. Buchan. 1991. Tuberculosis in domesticated red deer: comparison of purified protein derivative and the specific protein MPB70 for in vitro diagnosis. Res. Vet. Sci. 50:279-285.
14. Harboe, M., S. Nagai, M. E. Patarroyo, M. L. Torres, C. Ramirez, and N. Cruz. 1986. Properties of proteins MPB64, MPB70 and MPB80 of Mycobacterium bovis BCG. Infect. Immun. 52:293-302.
15. Harris, D. P., H. M. Vordermeier, S. J. Brett, G. Psavol, C. Moreno, and J. Ivanyi. 1994. Epitope specificity and isoforms of the mycobacterial 19-kilodalton antigen. Infect. Immun. 62:2963-2972.
16. Harris, D. P., H. M. Vordermeier, G. Friscia, H.-M. Surcel, G. Psavol, C. Moreno, and J. Ivanyi. 1993. Genetically permissive recognition of adjacent epitopes from the 19 kDa antigen of Mycobacterium tuberculosis by human and murine T cells. J. Immunol. 150:5041-5050.
17. Harris, D. P., H. M. Vordermeier, E. Roman, R. Lathigra, S. J. Brett, C. Moreno, and J. Ivanyi. 1991. Murine T cell stimulatory peptides from the 19 kDa antigen of Mycobacterium tuberculosis: epitope-restricted homology with the 28 kDa protein of Mycobacterium leprae. J. Immunol. 147:2706-2712.
18. Herber-Katz, E., R. H. Schwartz, L. A. Matis, C. Hannum, T. Fairwell, E. Appella, and D. Hansburg. 1982. Contribution of antigen-presenting cell major histocompatibility complex gene products to the specificity of antigen-induced T cell activation. J. Exp. Med. 155:1086-1089.
19. Hewinson, R. G., and W. P. Russell. 1993. Processing and secretion by Escherichia coli of a recombinant form of the immunogenic protein MPB70 of Mycobacterium bovis. J. Gen. Microbiol. 139:1253-1259.
20. Hochstrasser, D. F., M. G. Harrington, A.-C. Hochstrasse, M. J. Miller, and C. R. Merril. 1988. Methods for increasing the resolution of two-dimensional protein electrophoresis. Anal. Biochem. 173:424-435.