Development of an antigen capture enzyme immuno assay using genus specific monoclonal antibodies for detection of Chlamydia in clinical specimens

Indian Journal of Medical Research

Volumn 103, Issue FEB., 1996, Pages 77-83

  Mohanty, Sujata ^a   Satpathy, Gita ^a   Mittal, Suneeta ^a   Panda, S K ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Cervicitis; Direct immunofluorescence assay; Enzyme immuno assay; Follicular conjunctivitis; Group specific monoclonal antiboay; Major outer membrane protein antigen; Rabbit polyclonal antibody; Sensitivity; Specificity

Indexed keywords

BACTERIAL ANTIGEN; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; OUTER MEMBRANE PROTEIN;

ADOLESCENT; ADULT; ANTIGEN DETECTION; ARTICLE; BACTERIUM DETECTION; CHILD; CHLAMYDIA TRACHOMATIS; CHLAMYDIACEAE; CONJUNCTIVITIS; ENZYME IMMUNOASSAY; FEMALE; HUMAN; IMMUNOFLUORESCENCE TEST; MAJOR CLINICAL STUDY; PRESCHOOL CHILD; SCHOOL CHILD; UTERINE CERVICITIS;

ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; ANTIGENS, BACTERIAL; BACTERIAL OUTER MEMBRANE PROTEINS; BIOLOGICAL ASSAY; CHLAMYDIA TRACHOMATIS; FEMALE; HUMANS; IMMUNOENZYME TECHNIQUES; SPECIES SPECIFICITY;

EID: 0030041737     PISSN: 09715916     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. <1. Schachter J. Overview of human disease. In : Barron AL, editor. Microbiology of Chlamydia. Florida : CRC Press Inc. 1988; 153-65.
2. Freedom from Trachoma : A practical approach : Program for Tropical Disease Research, New York : Edna McConnel Clark foundation. 1994; 7-9.
3. Centre for Disease Control and Prevention : Recomendation for the prevention and management of C. trachomatis infection. MMWR 1993; 42 RR-12 : 1-36.
4. Grayston JT, Kuo CC, Campbell LA, Wang SP. Chlamydia pnenmoniae sp. nov. for Chlamydia sp. strain TWAR. Int J Syst Bacteriol 1989; 39 : 88-90.
5. Thomas BJ, Evans RT, Hawkins DA, Taylor Robinson D. Sensitivity of detecting Chlamydia trachomatis elementary bodies in smears by use of fluorescein labelled monoclonal antibody : comparison with conventional chlamydial isolation. J Clin Pathol 1984; 37 : 812-6.
6. Ridgway GL, Taylor Robinson D. Current problems in microbiology : 1. Chlamydial infections : which laboratory test. J Clin Pathol 1991; 44 : 1-5.
7. Mumtaz G, Mellars BJ, Ridgway GL, Oriel JD. Enzyme immunoassay for the detection of Chlamydia trachomatis antigen in urethral and endocervical swabs. J Clin Pathol 1985; 38 : 740-2.
8. Thomas BJ. MacLeod EJ, Taylor Robinson D. Evaluation of sensitivity of 10 diagnostic assays for Chlamydia trachomatis by use of a simple laboratory procedure. J Clin Pathol 1993; 46 : 912-4.
9. Satpathy G, Mohanty S, Panda SK. Development & use of antichlamydial monoclonal antibodies for rapid diagnosis of follicular conjunctivitis. Indian J Med Res 1994; 99 : 206-11.
10. Treharne JD, Darougar S, Jones BR. Modification of the micro-immunofluoresoence test to provide a routine serodiagnostic test for Chlamydial infections. J Clin Pathol.
11. Harlow ED, Lone D. Antibodies : A laboratory mannual. New York : Cold Spring Harbor Laboratory Publication. 1988 : 289-99.
12. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193 : 265-76.
13. Caldwell HD, Kromhout J, Schachter J. Purification & partial characterisation on the major outer membrane protein of Chlamydia trachomatis. Infect Immun 1981; 31 : 1161-76.
14. Redy KS. How to evaluate diagnostic tests. Natl Med J India 1989; 2 : 83-8.
15. Woods GL, Bryan JA. Detection of Chlamydia trachomatis by direct fluorescent antibody staining. Arch Pathol Lab Med 1994; 118 : 483-8.
16. Tjiam KH, van Heijst BYM, Van Zuuren A, Wagenvoort JHT, Van Joost T, Stalz E. et al. Evaluation of an enzyme immunoassay for the diagnosis of Chlamydial infections in.urogenital specimens. J Clin Microbiol 1986; 23 : 752-4.
17. Goudswaard J, Sabbe L, Van Belzen C. Interference by Gram negative bacteria in the enzyme immuno assay for detecting Chlamydia trachomatis. J Infect 1989; 18 : 94-6.
18. Saikku P, Puolakkainen M, Leinonen M, Nurminen M, Nissinen A. Cross reactivity between Chlamydiazyme and Acinetobacter strains. N Engl J Med 1986; 314 : 922-23.
19. Wilson MC, Millon-Velasco F, Tielsch JM, Taylor HR. Direct smear fluorescent antibody cytology as field diagnostic tool for trachoma. Arch Ophthamol 1986; 104 : 688-90.
20. Barsoum IS, Mostafa MSE, Shihab AA, Alamy ME, Habib MA, Colley DG. Prevalence of trachoma in school children and ophthamological outpatients in rural Egypt. Am J Trop Med Hyg 1987; 36 : 97-101.
21. Mabey DCW, Robertson JN, Ward ME. Detection of Chlamydia trachomatis by enzyme immunoassay in patients with trachoma. Lancet 1987; ii : 1491-2.
22. Mittal A, Kapur S, Gupta S. Chlamydial cervicitis : Role of culture, enzyme immuno assay and Geimsa cytology in diagnosis. APMIS 1993; 101 : 37-40.
23. Uyeda CT, Welborn P, Ellison Birang N, Shunk K, Tsaoux B. Rapid diagnosis of Chlamydial infections with Microtrak Direct Test. J Clin Microbiol 1984; 20 : 948-50.
24. Coudron PE, Fedorko DP, Dawson MS, Kaplowitz LG, Brookman RR, Dalton HP et al. Detection of Chlamydia trachomatis in genital specimens, by the Microtrak Direct specimen test. Am J Clin Pathol 1986; 85 : 89-92.
25. Thomas BJ, Osborn MF, Gilchrist C, Taylor Robinson D. Improved sensitivity of an enzyme immunoassay IDEIA for detecting Chlamydia trachomatis. J Clin Pathol 1989; 42 : 759-62.
26. Taylor Robinson D, Thomas BJ, Osborn MF. Evaluation of enzyme immunoassay (Chlamydiazyme) for detecting Chlamydia trachomatis in genital tract specimens. J Clin Pathol 1987; 40 : 194-9.
27. Smith JW, Rogers RE, Katz BP, Brickler JF, Lineback RL, VanDerpol B et al. Diagnosis of Chlamydia infection in women attending antenatal and gynaecology clinics. J Clin Microbiol 1987; 25 : 868-72.
28. Kellogg JA, Seiple JW, Hick ME. Cross-reaction of clinical isolates of bacteria and yeasts with the chlamydiazyme test for chlamydial antigen, before and after use of a blocking reagent. Am J Clin Pathol 1992; 97 : 309-12.