Covalent tethering of the dimer interface annuls aggregation in thymidylate synthase

Protein Science

Volumn 5, Issue 2, 1996, Pages 270-277

  Agarwalla, Sanjay ^a   Gokhale, Rajesh S ^a   Santi, Daniel V ^b   Balaram, P ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

intermolecular disulfides; protein aggregation annulment; size exclusion chromatography; thymidylate synthase

Indexed keywords

THYMIDYLATE SYNTHASE; UREA;

ARTICLE; CONCENTRATION RESPONSE; ENZYME SUBUNIT; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN CROSS LINKING; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; STRUCTURE ANALYSIS;

EID: 0030041283     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560050211     Document Type: Article

References (37)

1. Bennet MJ, Choe S, Eisenberg D. 1994. Domain swapping: Entangling alliances between proteins. Proc Natl Acad Sci USA 91:3127-3131.
2. Bradshaw TP, Dunlap BR. 1979. Characterization of the covalent chromatography of thymidylate synthase on thiopropyl-sepharose 6B. Biochim Biophys Acta 1163:165-175.
3. Brems DN, Alter LA, Beckage MJ, Chance RE, DiMarchi RD, Long HB, Pekar AH, Shields JE, FranK JH. 1992. Altering the association properties of insulin by amino acid. Protein Eng 5:527-533.
4. Brems DN, Plaisted SM, Kauffman EW, Havel HA. 1986. Characterization of an associated equilibrium folding intermediate of bovine growth hormone. Biochemistry 25:6539-5643.
5. Bychkova VE, Ptitsyn OB. 1995. Folding intermediates are involved in genetic diseases? FEBS Lett 359:3-5.
6. Come JH, Lansbury PT Jr. 1994. Predisposition of prion protein homozygotes to Creutzfeldt-Jacob disease can be explained by a nucleation-dependent polymerization mechanism. J Am Chem Soc 116:4109-4110.
7. Corbett RJT, Roche RS. 1984. Use of high speed size-exclusion chromatography for the study of protein folding and stability Biochemistry 23: 1888-1894.
8. Creighton TE. 1983. An empirical approach to protein conformation stability and flexibility. Biopolymers 22:49-58.
9. Creighton TE. 1992. Protein folding. W.H. Freeman & Co.
10. Crestfield AM, Stein WH, Moore S. 1962. On the aggregation of bovine pancreatic ribonuclease. Arch Biochem Biophys(Suppl 1):217-233.
11. DeYoung LR, Dill KA, Fink AL. 1993a. Aggregation and denaturation of apomyoglobin in aqueous urea solutions. Biochemistry 32:3877-3886.
12. DeYoung LR, Fink A, Dill KA. 1993b. Aggregation of globular proteins. Acc Chem Res 26:614-620.
13. Fields GB, Alomo DOV, Stigler D, Dill KA. 1992. Theory of aggregation of proteins and copolymers. J Phys Chem 96:3974-3981.
14. Fish WW, Mann KG, Tanford C. 1969. The estimation of polypeptide chain molecular weights by gel filtration in 6 M guanidine hydrochloride. J Biol Chem 244:4989-4994.
15. Fish WW, Reynolds JA, Tanford C. 1970. Gel chromatography of proteins in denaturing solutions. J Biol Chem 245:5166-5168.
16. Garel JR. 1992. Folding of large proteins. In: Creighton TE, ed. Protein folding. W.H. Freeman & Co. pp 405-454.
17. Gasset M, Baldwin MA, Lloyd DH, Gabriel JM, Holtzmann DM, Cohen F, Fletterick R, Prusiner SB. 1992. Predicted α-helical regions of the prion protein when synthesized as peptides form amyloids. Proc Natl Acad Sci USA 89:10940-10944.
18. Gokhale RS, Agarwalla S, Francis VS, Santi DV, Balaram P. 1994. Thermal stabilization of thymidylate synthase by engineering two disulfide bridges across the dimer interface. J Mol Biol 235:89-94.
19. Goldenberg DP, Creighton TE. 1984. Gel electrophoresis in studies of protein conformation and folding. Anal Biochem 138:1-18.
20. Hardy LW, Finner-Moore JS, Montford WR, Jones MO, Santi DV, Stroud RM. 1987. Atomic structure of thymidylate synthase: Target for rational drug design. Science 235:448-455.
21. Havel HA, Kauffman EW, Plaisted SM, Brems DN. 1986. Reversible self-association of bovine growth hormone during equilibrium unfolding. Biochemistry 25:6533-6538.
22. Horowitz PM, Butler M. 1993. interactive intermediates are formed during the urea unfolding of rhodanase. J Biol Chem 268:2500-2504.
23. Jaenicke R. 1987. Folding and association of proteins. Prog Biophys Mol Biol 49:117-237.
24. Kraulis PJ. 1991. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures. J Appl Crystallogr 24:946-950.
25. Lammeli UK. 1970. Cleavage of structural proteins during the assembly of the bacteriophage T4. Nature 277:680-685.
26. Lehrman RS, Tuls JL, Havel HA, Haskell RJ, Putnam SD, Tomich CSC. 1991. Site-directed mutagenesis to probe protein folding: Evidence that the formation and aggregation of a bovine growth hormone folding intermediate are dissociable processes. Biochemistry 30:5777-5784.
27. London J, Skrzynia C, Goldberg ME. 1974. Renaturation of E. coli tryptophanase after exposure to 8 M urea. Evidence for the existence of multinucleation centeres. Eur J Biochem 47:409-415.
28. Makhatadze GI, Privalov PL. 1992. Protein interactions with urea and guanidinium chloride, a calorimetric study. J Mol Biol 226:491-505.
29. Perry KM, Pookanjanatavip M, Zhao J, Santi DV, Stroud RM. 1992. Reversible dissociation and unfolding of the dimeric protein thymidylate synthase. Protein Sci 1:796-800.
30. Pohl M, Grotzinger J, Wollmer A, Kula M. 1994. Reversible dissociation and unfolding of pyruvate decarboxylase from Zymomonas mobilis. Eur J Biochem 224:651-661.
31. Pookanjanatavip M, Yuthavong Y, Greene PJ, Santi DV. 1992. Subunit complementation of thymidylate synthase. Biochemistry 31:10303-10309.
32. Prusiner SB. 1991. Molecular biology of prion diseases. Science 252:1515-1518.
33. Reinsch JW, Smith LL, Dunlap RB. 1979. Denaturation of thymidylate synthase from amethopterin-resistant Lactobacillus casei. Cancer Biochem Biophys 3:57-64.
34. Schein CH. 1989. Production of soluble recombinant protein in bacteria. Biotechnology 7:1141.
35. Sowdhamini R, Srinivasan N, Shoichet B, Santi DV, Ramakrishnan C, Balaram P. 1989. Stereochemical modelling of disulfide bridges. Criteria for introduction into proteins by site-directed mutagenesis. Protein Eng 3:95-103.
36. Srinivasan N, Sowdhamini R, Ramakrishnan CR, Balaram P. 1990. Conformation of disulfide bridges in proteins. Int J Peptide Protein Res 36:147-157.
37. Tanford C, Nozaki Y, Reynolds JA, Makino S. 1974. Molecular characterization of proteins in detergent solutions. Biochemistry 13:2369-2376.