Mechanistic studies on thiaminase I. Overexpression and identification of the active site nucleophile

Journal of Biological Chemistry

Volumn 271, Issue 7, 1996, Pages 3445-3452

  Costello, C A ^a   Kelleher, N L ^a   Abe, M ^a   McLafferty, F W ^a   Begley, T P ^a  

^a Department of Molecular Biology and Genetics   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

THIAMINE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

ALKYL AND ARYL TRANSFERASES; AMINO ACID SEQUENCE; BACILLUS; BASE SEQUENCE; BINDING SITES; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, DEAE-CELLULOSE; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENES, BACTERIAL; KINETICS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OPEN READING FRAMES; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; RESTRICTION MAPPING; TRANSFERASES;

EID: 0030041097     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.7.3445     Document Type: Article

References (0)