Simple 5′ extension method allowing transcript discrimination

BioTechniques

Volumn 20, Issue 1, 1996, Pages 24-26

  Breda, Colette ^a   El Turk, Joumana ^a   Esnault, Robert ^a  

^a INSTITUT DES SCIENCES DU VÉGÉTAL   (France)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA;

AMINO ACID SEQUENCE; ARTICLE; GENE AMPLIFICATION; GENETIC TRANSCRIPTION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT; POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; BACTERIOPHAGE LAMBDA; BASE SEQUENCE; BIOTECHNOLOGY; DNA PRIMERS; DNA, COMPLEMENTARY; DNA, PLANT; GENES, PLANT; GENETIC TECHNIQUES; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; POLYMERASE CHAIN REACTION;

EID: 0030040631     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96201bm03     Document Type: Article

References (9)

1. Edwards, J.B., J. Delort and J. Mallet. 1991. Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5′ ends of mRNAs and for constructing cDNA libraries by in vitro amplification. Nucleic Acids Res. 19.5227-5232.
2. Esnault, R., D. Buffard, C. Breda, C. Sallaud, J. El Turk and A. Kondorosi. 1993. Pathological and molecular characterizations of alfalfa interactions with compatible and incompatible bacteria, Xanthomonas campestris pv. alfalfae and Pseudomonas syringae pv ptsi. Molecular Plant-Microbe Interactions 6:655-664.
3. Esnault, R., J. El Turk, O. Asemota, C. Sallaud, C. Breda, D. Buffard and A. Kondorosi. 1993. Structure and expression of several peroxidase cDNAs from Medicago sativa, p. 361-368. In K.G. Welinder, S.K. Rasmussen, C. Penel and H. Greppin (Eds.), Plant Peroxidases: Biochemistry and Physiology. University of Geneva, Geneva.
4. Frohman, M.A., M.K. Dush and G.R. Martin. 1988. Rapid production of full-length cDNAs from rare transcripts: amplification using single gene-specific oligonucleotide primer. Proc. Natl. Acad Sci. USA 55:8998-9002
5. Harvey, R.J. and M.G. Darlison. 1991. Random-primed cDNA synthesis facilitates the isolation of multiple 5′-cDNA ends by RACE. Nucleic Acids Res. 19:4002.
6. Sallaud, C., J. El Turk, L. Bigarré, H. Sevin, R. Welle and R. Esnault. Nucleotide sequences of three chalcone reductase genes from Medicago sativa Plant Physiol. (In press).
7. Towner, P. and W. Gärtner. 1992. cDNA cloning of 5′ terminal regions. Nucleic Acids Res. 20:4669-4670.
8. Troutt, A.B., M.G. McHeyzer-Williams, B. Pulendram and G.J.V. Nossal. 1992. Ligation-anchored PCR: a simple amplification technique with single-sided specificity. Proc. Natl. Acad. Sci. USA 89:9823-9825.
9. Zeiner, M. and U. Gehring. 1994. Cloning of 5′ cDNA regions by inverse PCR. BioTechniques 17 1051-1053.