Characterization of Brucella abortus and Brucella melitensis native haptens as outer membrane O-type polysaccharides independent from the smooth lipopolysaccharide

Journal of Bacteriology

Volumn 178, Issue 4, 1996, Pages 1070-1079

  Aragon V ^a   Diaz R ^a   Moreno, E ^b   Moriyon I ^a,c  

^a UNIVERSITY CLINIC OF NAVARRA   (Spain)

^b UNIVERSIDAD NACIONAL   (Costa Rica)

^c UNIVERSITY OF NAVARRA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

HAPTEN; LIPOPOLYSACCHARIDE; POLYSACCHARIDE;

ARTICLE; BACTERIAL GROWTH; BRUCELLA ABORTUS; BRUCELLA MELITENSIS; CARBOHYDRATE ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNE RESPONSE; IMMUNOPRECIPITATION; NONHUMAN; OUTER MEMBRANE; PRIORITY JOURNAL; SEROLOGY; THIN LAYER CHROMATOGRAPHY;

EID: 0030039647     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.4.1070-1079.1996     Document Type: Article

References (56)

1. Alton, G. G., L. M. Jones, R. D. Angus, and J. M. Verger. 1988. Techniques for the brucellosis laboratory. Institut de la Recherche Agronomique, Paris.
2. Aragón, V., I. Moriyón, E. Moreno, and R. Díaz. Unpublished results.
3. Bendayan, M., and M. Zollinger. 1983. Ultrastructural location of antigenic sites on osmium-fixed tissues applying the protein A-gold technique J. Histochem Cytochem. 31:101-109.
4. Berman, D. T., B. L. Wilson, E. Moreno, R. D. Angus, and L. M. Jones. 1980. Characterization of Brucella abortus soluble antigen employed in immunoassay J. Clin. Microbiol 11:355-362.
5. Beynon, L. M., A. J. Dumanski, R. J. C. McLean, L. L. MacLean, J. C. Richards, and M. B. Perry. 1992. Capsule structure of Proteus mirabilis (ATCC 49565). J. Bacteriol. 174:2172-2177.
6. Breedveld, M. W., and K. J. Miller. 1994 Cyclic β-glucans of members of the family Rhizobiaceae Microbiol. Rev 58:145-161
7. Bundle, D. R., J. W. Cherwonogrodzky, and M. B. Perry. 1987. The structure of the lipopolysaccharide O-chain (M-antigen) and polysaccharide B produced by Brucella meluensis 16M. FEMS Microbiol Lett. 216:261-264.
8. Caroff, M., D. R. Bundle, M. B. Perry, J. W. Cherwonogrodzky, and J. R. Duncan. 1984. Antigenic S-type lipopolysaccharide of Brucella abortus 1119-3 Infect Immun. 46:384-389.
9. Cherwonogrodzky, J. W., G. Dubray, E. Moreno, and H. Mayer. 1990 Antigens of Brucella, p. 19-64 In K. Nielsen, and J. R. Duncan (ed.), Animal brucellosis CRC Press, Inc., Boca Raton, Fla.
10. Diaz, R., P. Garatea, L. M. Jones, and I. Moriyón. 1979. Radial immunodiffusion test with a Brucella polysaccharide antigen for differentiating infected from vaccinated cattle. J. Clin. Microbiol. 10:37-41.
11. Díaz, R., L. M. Jones, D. Leong, and J. B. Wilson. 1968. Surface antigens of smooth brucellae. J. Bacteriol. 96:893-901.
12. Díaz, R., and I. Moriyón. 1989. Laboratory techniques in the diagnosis of human brucellosis, p. 73-83. In E J. Young, and M. J Corbel (ed.). Brucellosis: clinical and laboratory aspects of human infection CRC Press, Inc., Boca Raton, Fla.
13. Díaz, R., J. Toyos, M. D. Salvo, and M. L. Pardo. 1981. A simple method for the extraction of polysaccharide B from Brucella cells for use in the radial immunodiffusion test diagnosis of bovine brucellosis. Ann. Rech. Vet. 12: 35-39.
14. Díaz-Aparicio, E., V. Aragón, C. Marín, B. Alonso, M. Font, E. Moreno, S. Pérez-Ortiz, J. M. Blasco, R. Díaz, and I. Moriyón. 1993. Comparative analysis of Brucella serotype A and M serotype and Yersinia enterocolitica O 9 polysaccharides for the serological diagnosis of brucellosis in cattle, sheep, and goats. J. Clin. Microbiol 31:3136-3141
15. Díaz-Aparicio, E., C. M. Marín, B. Alonso-Urmeneta, V. Aragón, S. Pérez-Ortiz, M. Pardo, J. M. Blasco, R. Díaz, and I. Moriyón. 1994. Evaluation of serological tests for the diagnosis of Brucella melitensis infection of goats J. Clin. Microbiol. 32:1159-1165.
16. Dranovskaja, E. A., and P. A. Versilova. 1971. Biochemical characteristics, antigenic structure and immunobiological properties of polysaccharide iractions of Brucella endotoxin. WHO document Bruc/71. 327. World Health Organization, Geneva.
17. Dubray, G. 1976. Localisation cellulaire des polyosides des bactéries des genres Brucella et Escherichia en phase lisse (S) or rugueuse (R). Ann. Inst. Pasteur Microbiol. 127B:133-149.
18. Fekete, A., J. A. Bantle, S. M. Halling, and R. W. Stich. 1992. Amplification fragment length polymorphism in Brucella strains by use of polymerase chain reaction with arbitrary primers. J. Bacteriol. 174:7778-7783.
19. Fernández-Lago, L., I. Moriyón, J. Toyos, and R. Díaz. 1982. Immunological identity of Brucella native hapten, polysaccharide B and Yersinia enterocolitica serotype 9 native hapten. Infect. Immun. 38:778-780.
20. Fox, J. D., and J. F. Robyt. 1991. Miniaturization of three carbohydrate analyses using a microsample plate reader Anal. Biochem. 195:93-96.
21. Gamazo, C., and I. Moriyón. 1987. Release of outer membrane fragments by exponentially growing Brucella melitensis cells. Infect. Immun. 55:609-615
22. Gamazo, C., A. I. Vitas, I. Moriyón, I. López-Goñi, and R. Díaz. 1993. Brucella group 3 outer membrane proteins contain a heat-modifiable protein. FEMS Microbiol. Lett 112:141-146.
23. Goldman, R. C., D. White, F. Ørskov, P. D. Rick, M. S. Lewis, A. K. Bhattacharjee, and L. Leive. 1982. A surface polysaccharide of Escherichia coli O111 contains O-antigen and inhibits agglutination of cells by O-antiserum J Bacteriol. 151:1210-1221.
24. Hawkes, R., E. Niday, and J. Gordon. 1982. A dot-immunobinding assay for monoclonal and other antibodies Anal Biochem. 119:142-147.
25. Hoffmann, E. M., and J. J. Houle. 1986. Purification of nonlipopolysaccharide antigen from Brucella abortus during preparation of antigen used for indirect hemolysis test. J. Clin. Microbiol 24:779-784.
26. Jann, B., K. Jann, and G. Schmidt. 1970. Immunochemical studies of polysaccharide surface antigens of Escherichia coli O100.K?(B):H2 Eur J Biochem 15:29-39
27. Jiménez de Bagues, M. P., C. M. Marín, J. M. Blasco, I. Moriyón, and C. Gamazo. 1991 An ELISA with Brucella lipopolysaccharide antigen for the diagnosis of B. melitensis infection in sheep and for the evaluation of setological responses following subcutaneous or conjunctival B. melitensis Rev 1 vaccination. Vet Microbiol. 30:233-241
28. Jones, L. M., D. T. Berman, E. Moreno, B. L. Deyoe, M. J. Gilsford, J. D. Huber, and P. Nicoletti. 1980. Evaluation of a radial immunodiffusion test with polysaccharide B antigen for diagnosis of bovine brucellosis. J. Clin. Microbiol. 12:753-760.
29. Kogan, G., B. Jann, and K. Jann. 1992 Structure of the Escherichia coli O:104 polysaccharide and its identity with the capsular K9 polysaccharide. FEMS Microbiol. Lett. 91:135-140.
30. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
31. Leong, D., R. Díaz, K. Milner, J. Rudbach, and J. B. Wilson. 1970 Some structural and biological properties of Brucella endotoxin. Infect Immun 1:174-182
32. Lord, V. R., and J. W. Cherwonogrodzky. 1992. Evaluation of polysaccharide, lipopolysaccharide, and beta-glucan antigens in gel immunodiffusion tests for brucellosis in cattle. Am. J. Vet. Res. 53:389-391.
33. L'vov, V. L., V. E. Malikor, A. S. Shaskov, E. A. Dranovskaja, and B. A. Dmitriev. 1985. Somatic antigens of the genus Brucella structure of the O-specific polysaccharide chain of the lipopolysaccharide of Brucella melitensis. Bioorg. Khim 11:963-969
34. L'vov, V. L., G. N. Pluzhnikova, E. B. Lapina, A. S. Shashkov, S. A. Askerova, E. Malikov, E. A. Dranovskaya, and B. A. Dimitriev. 1987. Molecular nature of the polysaccharide B antigen from Brucella (poly-B). Bioorg Khim 13: 1070-1074
35. Markwell, M. A. K., S. M. Haas, L. L. Bieber, and N. E. Tolbert. 1978 A modification of the Lowry procedure to simplify protein determination in membrane lipoprotein samples. Anal. Biochem. 87:206-210.
36. Meikle, P. J., M. B. Perry, J. W. Cherwonogrodzky, and D. Bundle. 1989. Fine structure of A and M antigens from Brucella biovars Infect. Immun. 57:2822-2828.
37. Moreno, E., L. M. Jones, and D. T. Berman. 1984. Immunochemical characterization of rough Brucella lipopolysaccharides Infect Immun. 43:779-782
38. Moreno, E., H. Mayer, and I. Moriyón. 1987. Characterization of a native polysaccharide hapten from Brucella melitensis. Infect Immun 55:2850-2853.
39. Moreno, E., M. W. Pitt, L. J. Jones, G. G. Schurig, and D. T. Berman. 1979 Purification and characterization of smooth and rough lipopolysaccharides from Brucella abortus. J, Bacteriol. 138:361-369.
40. Moreno, E., S. L. Speth, L. M. Jones, and D. T. Berman. 1981. Immunochemical characterization of Brucella lipopolysaccharide and polysaccharides. Infect. Immun 31:214-222.
41. Moreno, E., E. Stackebrandt, M. Dorsch, J. Wolters, M. Busch, and H. Mayer. 1990. Brucella abortus 16S rRNA and lipid A reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class Proteobacteria J Bacteriol. 172:3569-3576.
42. Moriyón, I., and D. T. Berman. 1982. Effects of nonionic, ionic and dipolar ionic detergents and EDTA on Brucella cell envelopes. J. Bacteriol. 152:822-828.
43. Oakley, B. R., D. R. Kirsch, and N. R. Morris. 1980. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels Anal. Biochem 105:361-363.
44. Oberti, J., R. Caravano, and J. Roux. 1982. Demonstration of an external layer in several species of the genus Brucella Can J. Microbiol. 28:1300-1303.
45. Perera, V. Y., A. J. Winter, and B. Ganem. 1984. Evidence for covalent bonding of native hapten-protein complexes to smooth lipopolysaccharide of Brucella abortus. FEMS Microbiol. Lett. 21:263-266.
46. Perry, M. B., and D. R. Bundle. 1990 Lipopolysaccharide antigens and carbohydrates of Brucella, p. 76-88. In L G. Adams (ed), Advances in brucellosis research. Texas A&M University Press, College Station.
47. Peterson, A. A., and E. McGroarty. 1985. High-molecular-weight components in lipopolysaccharides of Salmonella typhimurium, Salmonella minnesota, and Escherichia coli. J. Bacteriol. 162:738-745
48. Redmond, J. W. 1979. The structure of the O-antigenic side chain of lipopolysaccharide of Vibrio cholerae 569B (INABA). Biochim. Biophys. Acta 584:346-352.
49. Ribi, E., W. T. Haskins, M. Landy, and K. Milner. 1961. Preparation and host-reactive properties of endotoxin with low content of nitrogen and lipid J. Exp. Med. 114:647-663.
50. Rick, P. D. 1987. Lipopolysaccharide biosynthesis, p. 648-662. In F C. Neidhardt, J. L Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology, vol. 1 American Society for Microbiology, Washington, D C.
51. Rojas, N., E. Freer, A. Weintraub, M. Ramírez, S. Lind, and E. Moreno. 1994. Immunochemical identification of Brucella abortus lipopolysaccharide epitopes. Clin. Diag. Lab. Immunol 1:206-213.
52. Sittenfeld, A., and E. Moreno. 1988. A sensitive blotting system for detection of alpha-feto protein variants with monoclonal and polyclonal antibodies J. Immunol. Methods 106:19-26.
53. Touchstone, J. C., and M. F. Dobbins. 1983. Practice of thin layer chromatography, 2nd ed. John Wiley & Sons Inc., New York.
54. Tsai, C., and C. E. Frasch. 1982. A sensitive stain for detecting lipopolysaccharides in polyacrylamide gels Anal. Biochem. 119:115-119.
55. Wu, A. M., and N. E. Mackenzie. 1987. Structural and immunochemical characterization of the O-haptens of Brucella abortus lipopolysaccharides from strains 19 and 2308 Mol. Cell. Biochem. 75:103-111
56. Zygmunt, M. S., G. Dubray, D. R. Bundle, and M. B. Perry. 1988. Purified native haptens of Brucella abortus B19 and B, melitensis 16M reveal the lipopolysaccharide origin of the antigens Ann. Inst Pasteur/Microbiol. (Paris) 139:421-123