Molecular analysis of the amy gene locus of Thermoanaerobacterium thermosulfurigenes EM1 encoding starch-degrading enzymes and a binding protein-dependent maltose transport system

Journal of Bacteriology

Volumn 178, Issue 4, 1996, Pages 1039-1046

  Sahm, Kerstin ^a   Matuschek, Markus ^a   Müller, Harald ^a   Mitchell, Wilfrid J ^b   Bahl, Hubert ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b HERIOT WATT UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MALTOSE; STARCH;

ANAEROBIC BACTERIUM; ARTICLE; CARBOHYDRATE METABOLISM; CATABOLITE REPRESSION; ENZYME DEGRADATION; GENE EXPRESSION; GENE LOCATION; GENE LOCUS; GENETIC ANALYSIS; GLUCOSE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; THERMOPHILIC BACTERIUM;

EID: 0030039646     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.4.1039-1046.1996     Document Type: Article

References (43)

1. Angerer, A., S, Gaisser, and V. Braun. 1990 Nucleotide sequences of the sfuA, sfuB, and sfuC genes of Serratia marcescens suggest a periplasmic-binding-protein-dependent iron transport mechanism. J. Bacteriol 172:572-578.
2. Bahl, H., G. Burchhardt, A. Spreinat, K. Hacekel, A. Wienecke, B. Schmidt, and G. Antranikian. 1991. α-Amylase of Clostridium thermosulfurogenes EM1: nucleotide sequence of the gene, processing of the enzyme, and comparison to other α-amylases Appl Environ. Microbiol. 57:1554-1559.
3. Bahl, H., G. Burchhardt, and A. Wienecke. 1991. Nucleotide sequence of two Clostridium thermosulfurogenes EM1 genes homologous to Escherichia coli genes encoding integral membrane components of binding protein-dependent transport systems. FEMS Microbiol. Lett. 81:83-88.
4. Berish, S. A., D. R. Kapczynski, and S. A. Morse. 1990 Nucleotide sequence of the fbp gene from Neisseria meningitidis Nucleic Acids Res 18:4596
5. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
6. Chambliss, G. H. 1993. Carbon source-mediated catabolite repression, p. 213-219. In A. L. Sonenshein, J A. Hoch, and R Losick (ed.), Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology, and molecular genetics. American Society for Microbiology, Washington, D C.
7. Coleman, R. D., S.-S. Yang, and M. P. MacAlister. 1987. Cloning of the debranching-enzyme gene from Thermoanaerobium brocku into Escherichia coli and Bacillus subtitis. J. Bacteriol. 169:4302-4307.
8. Cook, M. C., P. H. Janssen, and H. W. Morgan. 1993 Uncoupler-resistant glucose uptake by the thermophilic glycolytic anaerobe Thermoanaerobacter thermohydrosulfuricus (Clostridium thermohydrosulfuricum). Appl. Environ. Microbiol 59:2984-2990.
9. Cook, M. C., P. H. Janssen, J. P. Russel, and H. W. Morgan. 1994. Dual mechanism of xylose uptake in the thermophilic bacterium Thermoanaerobacter thermohydrosulfuricus. FEMS Microbiol Lett. 116:257-262.
10. Dahl, M. K., E. Francoz, W. Saurin, W. Boos, M. D. Manson, and M. Hofnung. 1989. Comparison of sequences from the malB regions of Salmonella typhimurium and Enterobacter aerogenes with Escherichia coli K12: a potential new regulatory site in the interoperonic region. Mol Gen. Genet. 218:199-207.
11. Duplay, P., II. Bedouelle, A. Fowler, I. Zabin, W. Saurin, and M. Hofnung. 1984. Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K12 J. Biol Chem. 259:10606-10613
12. Gilson, E., G. Alloing, T. Schmidt, J.-P. Claverys, T. Dudler, and M. Hofnung. 1988. Evidence for high affinity binding protein-dependent transport systems in gram positive bacteria and in Mycoplasma. EMBO J 7:3971-3974
13. Golden, J. Personal communication
14. Hayashi, S., and H. C. Wu. 1990. Lipoproteins in bacteria J Bioenerg. Biomembr 22:451-471
15. Hernandez, P. E. 1982. Transport of D-glucose in Clostridium thermocellum ATCC-27405. J Gen. Appl. Microbiol. 28:469-477.
16. Higgins, C. F. 1992 ABC transporters from microorganisms to man Annu Rev Cell Biol. 8:67-113.
17. 16a. Hueck, C. J., W. Hillen, and M. H. Saier, Jr. 1994 Analysis of a cis-active sequence mediating catabolic repression in Gram-positive bacteria. Res. Microbiol. 145:503-518.
18. Hyun, H. H., and J. G. Zeikus. 1985. Regulation and genetic enhancement of glucoamylase and pullulanase production in Clostridium thermohydrosulfuricum. J. Bacteriol 164:1146-1152.
19. Kerppola, R. E., and G.F.-L. Ames. 1992. Topology of the hydrophobic membrane-bound components of the histidine periplasmic permease. Comparison with other members of the family. J. Biol. Chem. 267:2329-2336
20. Kimmich, G. A., J. Randles, and J. S. Brand. 1975 Assay of picomole amounts of ATP, ADP and AMP using luciferase enzyme system Anal. Biochem 69:187-206
21. Lacks, S. A., J. J. Dunn, and B. Greenberg. 1982 Identification of base mismatches recognized by the heteroduplex-DNA-repair system of Streptococcus pneumoniae. Cell 31:327-336.
22. Liebl, W., R. Feil, J. Gabelsberger, J. Kellermann, and K. Schleifer. 1992. Purification and characterization of a novel thermostable 4-α-glucanotransferase of Thermotoga maritima cloned in Escherichia coli. Eur. J. Biochem. 207:81-88
23. Madi, E. 1987. Characterization of an anaerobic, thermophilic bacterium and optimization of the conditions for the formation of its thermostable amylolytic enzymes Ph.D thesis University of Gottingen, Gottingen, Germany.
24. Madi, E., and G. Antranikian. 1989 Identification of a starch degrading anaerobic thermophile producing thermostable α-amylase and pullulanase. Appl. Microbiol Biotechnol. 30:422-425.
25. Matuschek, M., G. Burchhardt, K. Sahm, and H. Bahl. 1994. Pullulanase of Thermoanaerobacterium thermosulfurigenes EM1 (Clostridium thermosulfurogenes) molecular analysis of the gene, composite structure of the enzyme, and a common model for its attachment to the cell surface. J. Bacteriol. 176:3295-3302.
26. Matuschek, M., A. Zibat, K. Sahm, and H. Bahl. Unpublished results.
27. Melasniemi, H. 1988. Purification and some properties of the extracellular α-amylase-pullulanase produced by Clostridium thermohydrosulfuricum. Biochem. J. 250:813-818.
28. Melasniemi, H., M. Paloheimo, and L. Hemiö. 1990. Nucleotide sequence of the α-amylase-pullulanase gene from CLostridium thermohydrosulfuricum. J. Gen. Microbiol. 136:447-454.
29. Moran, C. P., Jr. 1993 RNA polymerase and transcription factors, p. 653-667. In A L Sonenshein, J. A. Hoch, and R Losick (ed.), Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology, and molecular Genetics. American Society for Microbiology, Washington, D.C.
30. Ng, T. K., and J. G. Zeikus. 1982. Differential metabolism of cellobiose and glucose by Clostridium thermocellum and Clostrdium thermohydrosulfuricum. J Bacteriol 150:1391-1399.
31. Oelmüller, U., N. Krüger, A. Steinbüchel, and C. G. Friedrich. 1990. Isolation of prokaryotic RNA and detection of specific mRNA with biotinylated probes. J. Microbiol. Methods 11:73-84
32. Overduin, P., W. Boos, and J. Tommassen. 1988. Nucleotide sequence of the ugp genes of Escherichia coli K-12: homology to the maltose system. Mol. Microbiol. 2:767-775.
33. Ramesh, M. V., M. Podkovyrov, S. E. Lowe, and J. G. Zeikus. 1994. Cloning and sequencing of the Thermoanaerobacterium saccharolyticum B6A-RI apu gene and purification and characterization of the amylopullulanase from Escherichia coli. Appl. Environ. Microbiol 60:94-101.
34. Russel, R. R. B., J. Aduse-Opoku, I. C. Sutcliffe, L. Tao, and J. J. Ferretti. 1992. A binding protein-dependent transport system in Streptococcus mutans responsible for multiple sugar metabolism. J. Biol. Chem. 267:4631-4637
35. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989 Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.
36. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors Proc Natl Acad. Sci. USA 74:5463-5467.
37. Schwartz, M. 1987. The maltose reguton, p. 1482-1502 In F. C. Neidhardt, J. L Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
38. Spreinat, A., and G. Antranikian. 1992. Analysis of the amylolytic enzyme system of Clostridium thermosulfurogenes EM1: purification and synergistic action of pullulanases and maltohexaose forming α-amylase. Staerke 44:305-312
39. Sutcliffe, I. C., L. Tao, J. J. Ferretti, and R. R. B. Russel. 1993. MsmE, a lipoprotein involved in sugar transport in Streptococcus mutans J Bacteriol 175:1853-1855.
40. Tam, R., and M. H. Saier, Jr. 1993. Structural, functional, and evolutionary relationship among extracellular solute-binding receptors of bacteria. Microbiol. Rev. 57:320-346
41. Tangney, M., P. Smith, F. G. Priest, and W. J. Mitchell. 1992. Maltose transport in Bacillus licheniformis NCIB 6346. J. Gen. Microbiol. 138:1821-1827.
42. v. Heijne, G. 1989. The structure of signal peptides from bacterial lipoproteins. Protein Eng. 2:531-534.
43. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors. Gene 33:103-119.