Enhanced α-amylase production in recombinant Bacillus brevis by fed-batch culture with amino acid control

Biotechnology and Bioengineering

Volumn 49, Issue 1, 1996, Pages 36-44

  Park, Yong Soo ^a   Dohjima, Takashi ^a   Okabe, Mitsuyasu ^a  

^a SHIZUOKA UNIVERSITY   (Japan)

Author keywords

Alpha amylase; Amino acid control; Bacillus brevis; Fed batch culture

Indexed keywords

AMINO ACID; AMYLASE;

AMINO ACID METABOLISM; ARTICLE; BACTERIAL METABOLISM; BACTERIUM CULTURE; BREVIBACILLUS BREVIS; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SYNTHESIS; GENETIC RECOMBINATION; NONHUMAN;

AMINO ACIDS; ANALYTIC EQUIPMENT; BATCH CELL CULTURE; COMPOSITION; CONTROL; ENZYME INHIBITION; IONS; MIXTURES; NITROGEN; PROTEINS; SUBSTITUTION REACTIONS; YEAST;

ALPHA-AMYLASE; AMMONIUM ION; ASPARAGINE; BACILLUS BREVIS; FED-BATCH CULTURE; FLASK CULTURE; ISOLEUCINE; NITROGEN SOURCE; POLYPEPTONE; YEAST EXTRACT;

GENETIC ENGINEERING;

EID: 0030039289     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1097-0290(19960105)49:1<36::aid-bit5>3.0.co;2-u     Document Type: Article

References (39)

1. Adachi, T , Yamagata, H., Tsukagoshi, N., Udaka, S. 1989. Multiple and tandemly arranged promoters of the cell wall protein gene operon in Bacillus brevis 47. J. Bacteriol. 171: 1010-1016.
2. Cayuela, C., Kai, K., Park, Y. S., Iijima, S , Kobayashi, T. 1993. Insecticide production by recombinant Bacillus subtilis 1A96 in fed-batch culture with control of glucose concentration. J. Ferment. Bioeng. 75: 383-386.
3. Doelle, H. W., Ewings, K. N., Hollywood, N. W. 1982. Regulation of glucose metabolism in bacterial systems. Adv. Biochem. Eng. 23: 1-35.
4. Doi, R. H. 1972. Role of proteases in sporulation. Curr. Top. Cell Regul. 6: 1-20.
5. Ebisu, S., Takagi, H , Kadowaki, K., Yamagata, H., Udaka, S. 1992. Production of human epidermal growth factor by Bacillus brevis increased with use of a stable plasmid from B. brevis 481. Biosci. Biotechnol. Biochem. 56: 812-813.
6. Heineken, F. G., O'Connor, R. J. 1975. Continuous culture studies on the biosynthesis of alkaline protease, neutral protease and α-amylase by Bacillus subtilis NRRL-B3411. J. Gen. Microbiol. 73: 35-44.
7. Iijima, S., Lin, K. H., Kobayashi, T. 1991. Increased production of cloned β-galactosidase in two-stage culture of Bacillus amylolique-faciens. J. Ferment. Bioeng. 71: 69-71.
8. Kole, M. M., Draper, I., Gerson, D. F. 1988. Protease production by Bacillus subtilis in oxygen-controlled, glucose fed-batch fermentation Appl. Microbiol. Biotechnol. 28: 404-408.
9. Konishi, H., Sato, T., Yamagata, H., Udaka, S. 1990. Efficient production of human α-amylase by a Bacillus brevis mutant. Appl. Microbiol. Biotechnol. 34: 297-302.
10. Konstantinov, K., Kishimoto, M., Seki, T., Yoshida, T. 1990. A balanced DO-stat and its application to the control of acetic acid excretion by recombinant Escherichia coli. Biotechnol. Bioeng. 36: 750-758.
11. Laishley, E. J., Bernlohr, R. W. 1968. Regulation of arginine and proline catabolism in Bacillus licheniformis. J. Bacteriol. 96: 322-329.
12. Luli, G. W., Schlasner, S. M., Ordaz, D. E., Mason, M., Strohl, W. R. 1987. An automatic, on-line glucose analyzer for feed-back control of fed-batch growth of Escherichia coli. Biotechnol. Tech. 1: 225-230.
13. Luli, G. W., Strohl, W. R. 1990. Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. Appl. Environ. Microbiol. 56: 1004-1011.
14. McCullough, H. 1967. The determination of ammonia in whole blood by a direct colorimetric method. Clin. Chim. Acta. 17: 297-304.
15. Miyashiro, S., Enei, H., Hirose, Y., Udaka, S. 1980. Effect of glycine and L-isoleucine on protein production by Bacillus brevis No. 47. Agric. Biol. Chem. 44: 105-112.
16. Mizukami, S., Yamagata, H., Sakaguchi, K., Udaka, S. 1992. Efficient production of thermostable Clostridium thermosulfurogenes β-amylase by Bacillus brevis. J. Ferment. Bioeng. 73: 112-115.
17. Mizutani, S., Iijima, S., Morikawa, M., Shimizu, K., Matsubara, M., Ogawa, Y., Izumi, R., Matsumoto, K., Kobayashi, T. 1987. On-line control of glucose concentration using an automatic glucose analyzer. J. Ferment Technol. 65: 325-231.
18. Moon, S. H., Parulekar, S. J. 1991. A parametric study of protease production in batch and fed-batch cultures of Bacillus firmus. Biotechnol. Bioeng. 37: 467-483.
19. Mori, H., Yano, T., Kobayashi, T., Shimizu, S. 1979. High density cultivation of biomass in fed-batch system with DO-stat. J Chem. Eng. Jpn. 12. 313-319
20. Park, Y S., Kai, K., Iijima, S., Kobayashi, T. 1992. Enhanced β-galactosidase production by high cell-density culture of recombinant Bacillus subtilis with glucose concentration control. Biotechnol. Bioeng. 40: 686-696.
21. Priest, F. G. 1975. Effect of glucose and cyclic nucleotides on the transcription of α-amylase mRNA in Bacillus subtilis. Biochem. Biophys. Res. Commun. 63: 606-610.
22. Roychoudhury, S., Parulekar, S. J., Weigand, W. A. 1989. Cell growth and α-amylase production characteristics of Bacillus amyloliquefaciens. Biotechnol. Bioeng. 33: 197-206.
23. Saito, N. 1973 A thermophilic extracellular α-amylase from Bacillus licheniformis. Arch. Biochem. Biophys. 155: 290-298.
24. Schaeffer, P. 1969. Sporulation and the production of antibiotics, exoenzymes, and exotoxins. Bacteriol. Rev. 33: 48-71.
25. Shaku, M., Koike, S., Udaka, S. 1980. Cultural conditions for protein production by Bacillus brevis No. 47. Agric. Biol. Chem. 44: 99-103.
26. Shimizu, K., Morikawa, M., Mizutani, S., Iijima, S., Matsubara, M., Kobayashi, T. 1988. Comparison of control techniques for Baker's yeast culture using an automatic glucose analyzer. J. Chem. Eng. Jpn. 21: 113-117.
27. Shimizu, N., Fukuzono, K., Fujimori, K., Nishimura, N., Odawara, Y. 1988 Fed-batch cultures of recombinant Escherichia coli with inhibitory substance concentration monitoring. J. Ferment. Technol. 66: 187-191.
28. Takao, M., Miyauchi, A., Kadowaki, K., Udaka, S. 1989. Potential use of Bacillus brevis HPD31 for the production of foreign proteins. Agric. Biol. Chem. 53: 2279-2280.
29. Tsuboi, A., Uchihi, R., Tabata, R., Takahashi, Y., Hashiba, H., Sasaki, T., Yamagata, H., Tsukagoshi, N., Udaka, S. 1986. Characterization of the genes coding for the two major cell wall proteins from protein-producing Bacillus brevis 47: Complete nucleotide sequence of the outer wall protein gene. J. Bacteriol. 168: 365-373.
30. Tsuboi, A., Uchihi, R., Adachi, T., Sasaki, T., Hayakawa, S., Yamagata, H., Tsukagoshi, N., Udaka, S. 1988. Characterization of the genes for the hexagonally arranged surface layer proteins in proteinproducing Bacillus brevis 47: Complete nucleotide sequence of the middle wall protein gene. J. Bacteriol. 170: 935-945.
31. Udaka, S. 1976. Screening for protein-producing bacteria. Agric. Biol. Chem. 40: 523-528.
32. Udaka, S., Tsukagoshi, N., Yamagata, H. 1989. Bacillus brevis, a host bacterium for efficient extracellular production of useful proteins. Biotechnol. Genet. Eng. Rev. 7: 113-145.
33. Udaka, S., Yamagata, H. 1993. High-level secretion of heterologous proteins by Bacillus brevis. Methods Enzyraol. 217: 23-33.
34. Wright, C. P., Whitney, G. K., Daugulis, A. J., White, B. N. 1992. Enhancement and regulation of extracellular protein production by Bacillus brevis 47 through manipulation of cell culture conditions. Biotechnol. Bioeng. 40: 46-52.
35. Yamada, H., Tsukagoshi, N . Udaka, S. 1981. Morphological alteration of cell wall concomitant with protein release in a proteinproducing bacterium. Bacillus brevis 47. J. Bacteriol. 148: 322-332.
36. Yamagata, H., Adachi, T., Tsuboi, A., Takao, M., Sasaki, T., Tsukagoshi, N., Udaka, S. 1987. Cloning and characterization of the 5′ region of the cell wall protein gene operon in Bacillus brevis 47. J. Bacteriol. 169: 1239-1245.
37. Yamagata, H., Nakahama, K., Susuki, Y., Kakinuma, A., Tsukagoshi, N., Udaka, S. 1989 Use of Bacillus brevis for efficient synthesis and secretion of human proteins. Proc. Natl. Acad. Sci. USA 86: 3589-3593.
38. Yano, T., Mori, H., Kobayashi, T , Shimizu, S. 1978. Fed-batch culture of methanol-utilizing bacterium with DO-stat. J. Ferment. Technol. 56: 416.
39. Zabriskie, D. W., Arcuri, E. J. 1986. Factors influencing productivity of fermentations employing recombinant microorganisms. Enzyme Microbiol. Technol. 8: 706-717.