Simplified probe preparation facilitates S1 nuclease analysis

BioTechniques

Volumn 20, Issue 2, 1996, Pages 174-178

  Noti, John D ^a   Reinemann, Bruce C ^a  

^a GUTHRIE RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; MESSENGER RNA; NUCLEASE S1; PHORBOL 13 ACETATE 12 MYRISTATE; SINGLE STRANDED DNA;

ARTICLE; CELL STRAIN HL 60; DNA PROBE; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION;

EID: 0030038490     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96202bm02     Document Type: Article

References (5)

1. Berk, A.J. and P.A. Sharp. 1977. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12:721-732.
2. 32P) DNA probes synthesized from bacteriophage m13mp templates. Gene 30:63-68.
3. Kaltenboeck, B., J.W. Spatafora, X. Zhang, K.G. Kousoulas, M. Blackwell and J. Storz. 1992. Efficient production of single-stranded DNA as long as 2 kb for sequencing of PCR-amplified DNA. BioTechniques 12:164-171.
4. Loez-Cabrera, M., A. Nueda, A. Vara, J. Garcia-Aguilar, A. Tugores and A. Corbi, 1993. Characterization of the p150,95 leukocyte integrin α subunit (CD1 1c) gene promoter. J. Biol. Chem. 268:1187-1193.
5. Sharrocks, A.D. and D.P. Hornby. 1991. S1 nuclease transcript mapping using Sequenasederived single-stranded probes. BioTechniques 10:426-428.