A recombinant single-chain HLA-A2.1 molecule, with a cis active β-2-microglobulin domain, is biologically active in peptide binding and antigen presentation

Human Immunology

Volumn 49, Issue 1, 1996, Pages 28-37

  Lee, Li ^a   Loftus, Douglas ^a   Appella, Ettore ^a   Margulies, David H ^b   Mage, Michael ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 2 MICROGLOBULIN; HLA A2 ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; MONOCLONAL ANTIBODY; PEPTIDE; RECOMBINANT PROTEIN; TAX PROTEIN;

ANIMAL CELL; ANTIGEN PRESENTATION; ARTICLE; CIS ISOMER; CONTROLLED STUDY; COVALENT BOND; CYTOTOXIC T LYMPHOCYTE; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FOLDING;

EID: 0030037725     PISSN: 01988859     EISSN: None     Source Type: Journal    
DOI: 10.1016/0198-8859(96)00056-0     Document Type: Article

References (34)

1. Veronese FD, Arnott D, Barnaba V, Loftus DJ, Sakaguchi K, Thompson CB, Salemi S, Mastroianni C, Sette A, Shabanowitz J, Hunt DF, Appella EA: Auto-reactive cytotoxic T lymphocytes in HIV-1 infected subjects. J Exp Med (in press).
2. Lee L, McHugh L, Sakuma S, Loftus D, Appella E, Margulies DH, Mage M: Peptide-loaded recombinant single-chain HLA molecules as potential immunogens for T cells. In Vaccines 95: Molecular Approaches to the Control of Infectious Diseases. Chanock RM, Brown F, Ginsburg HS, Norby E, Eds. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1995.
3. Mottez E, Langlade-Demoyen P, Gournier H, Martinon F, Maryanski J, Kourilski P, Abastado JP: Cells expressing a major histocompatibility complex class I molecule with a single covalently bound peptide are highly immunogenic. J Exp Med 181:493, 1995.
4. Kozono H, White J, Clements J, Marrack P, Kappler J: Production of soluble MHC class II proteins with covalently bound single peptides. Nature 369:151, 1994.
5. Ignatowicz L, Winslow G, Bill J, Kappler J, Marrack P: Cell surface expression of class II MHC proteins bound by a single peptide. J Immunol 154:3852, 1995.
6. Mage MG, Lee L, Ribaudo RK, Corr M, Kozlowski S, McHugh L, Margulies DH: A recombinant, soluble, single-chain class I major histocompatibility complex molecule with biological activity. Proc Natl Acad Sci USA 89:10658, 1992.
7. 2-microglobulin domain. Eur J Immunol 24:2633, 1994.
8. 2 Microglobulin/peptide complex assembled from subunits expressed separately in Escherichia coli. Mol Immunol 29:371, 1992.
9. Parker KC, Wiley DC: Overexpression of native human beta 2-microglobulin in Escherichia coli and its purification. Gene 83:117, 1989.
10. Gunning P, Leavitt J, Muscat G, Ng S-Y, Kedes L: A human beta-actin expression vector system directs high-level accumulation of antisense transcripts. Proc Natl Acad Sci USA 84:4831, 1987.
11. Winter CC, Carreno BM, Turner RV, Koenig S, Biddison WE: The 45 pocket of HLA-A2.1 plays a role in presentation of influenza virus matrix peptide and alloantigens. J Immunol 146:3508, 1991.
12. d mouse major histocompatibility antigen genes in L cells after DNA-mediated gene transfer. J Immunol 130:463, 1983.
13. Silverman T, Rein A, Orrison B, Langloss J, Bratthauer G, Miyazaki J, Ozato K: Establishment of cell lines from somite stage mouse embryos and expression of major histocompatibility class I genes in these cells. J Immunol 140:4378, 1988.
14. del Senno L, Anzanel D, Barbieri D, Barbieri P, Buzzoni D, Rossi P, Piva R, Gambari R: Human KJ29 cell line, isolated from a kidney adenocarcinoma: morphological as-pects and effects of chemical inducers. Cell Biol Int Rep 10:195, 1986.
15. Henderson RA, Cox AL, Sakaguchi K, Appella E, Shabanowitz J, Hunt DF, Engelhard VH: Direct identification of an endogenous peptide recognized by multiple HLA-A2.1-specific cytotoxic T cells. Proc Natl Acad Sci USA 90:10275, 1993.
16. Utz U, Koenig S, Coligan JE, Biddison WE: Presentation of three different viral peptides, HTLV-1 Tax, HCMV gB, and influenza virus M1, is determined by common structural features of the HLA-A2.1 molecule. J Immunol 149:214, 1992.
17. Brodsky FM, Parham P, Barnstable CJ, Crumpton MJ, Bodmer WE: Monoclonal antibodies for analysis of the HLA system. Immunol Rev 47:3, 1979.
18. Ellis SA, Taylor C, McMichael A: Recognition of HLA-B27 and related antigen by a monoclonal antibody. Hum Immunol 5:49, 1982.
19. Ozato K, Mayer NM, Sachs DH: Monoclonal antibodies to mouse major histocompatibility complex antigens. Transplantation 34:113, 1982.
20. Ruppert J, Sidney J, Celis E, Kubo RT, Grey HM, Sette A: Prominent role of secondary anchor residues in peptide binding to HLA-A*0201 molecules. Cell 74:929, 1993.
21. Hunt DF, Henderson RA, Shabanowitz J, Sakaguchi K, Michel H, Seilir N, Cox AL, Appella E, Engelhard VH: Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry. Science 255:1261, 1992.
22. Tsuchida T, Parker KC, Turner RV, McFarland HF, Coligan JE, Biddison WE: Autoreactive CD8+ T-cell responses to human myelin protein-derived peptides. Proc Natl Acad Sci USA 91:10859, 1994.
23. Gotch F, Rothbard J, Howland K, Townsend A, McMichael A: Cytotoxic T lymphocytes recognize a fragment of influenza virus matrix protein in association with HLA-A2. Nature 326:881, 1987.
24. del Guercio MF, Sidney J, Hermanson G, Perez C, Grey HM, Kubo RT, Sette A: Binding of a peptide antigen to multiple HLA alleles allows definition of an A2-like supertype. J Immunol 154:685, 1995.
25. Bylund DB, Yamamura HI: Methods for receptor binding. In Yamamura HI et al (eds): Methods in Neurotransmitter Receptor Analysis. New York, Raven Press, 1990.
26. Sette A, Sidney J, del Guercio M-F, Southwood S, Ruppert J, Dahlberg C, Grey HM, Kubo RT: Peptide binding to the most frequent HLA-A class I alleles measured by quantitative molecular binding assays. Mol Immunol 31:813, 1994.
27. Kubo RT, Sette A, Grey HM, Appella E, Sakaguchi K, Zhu N-Z, Arnott D, Sherman N, Shabanowitz J, Michel H, Bodmer WM, Davis TA, Hunt DF: Definition of specific peptide motif for four major HLA-A alleles. J Immunol 152:3913, 1994.
28. Kast WM, Brandt RMP, Sidney J, Drijfhout J-W, Kubo RT, Grey HM, Melief CJM, Sette A: The role of HLA-A motifs in identification of potential CTL epitopes in human papillomavirus type 16 E6 and E7 proteins. J Immunol 152:3904, 1994.
29. Bertoletti A, Ferrari C, Fiaccadori F, Penna A, Margolskee R, Schkicht HJ, Fowler P, Guihot S, Chisari FV: HLA class I-restricted human cytotoxic T cells recognize endogenously synthesized hepatitis B virus nucleocapsid antigen. Proc Natl Acad Sci USA 88:10445, 1991.
30. Nayersina R, Fowler P, Guilhot S, Missale G, Cerny A, Schlicht H-J, Vitiello A, Chesnut R, Person JL, Redeker AG, Chisari FV: HLA-A2 restricted cytotoxic T lymphocyte responses to multiple hepatitis B surface antigen epitopes during hepatitis B virus infection. J Immunol 150:4659, 1993.
31. Bertoletti A, Chisari FV, Penna A, Guilhot S, Galati L, Missale G, Fowler P, Schlicht HJ, Vitiello A, Chesnut RW, Fiaccadori F, Ferrari C: Definition of a minimal optimal cytotoxic T cell epitope within the hepatitis B virus nucleocapsid protein. J Virol 67:2376, 1993.
32. Takahashi H, Nakagawa Y, Yokomuro K, Berzofsky JA: Induction of CD8+ cytotoxic T lymphocytes by immunization with syngeneic irradiated HIV-1 envelope derived peptide-pulsed dendritic cells. Int Immunol 5:849, 1993.
33. Pogue RR, Eron J, Frelinger JA, Matsui M: Aminoterminal alteration of the HLA-A*0201-restricted human immunodeficiency virus pol peptide increases complex stability and in vitro immunogenicity. Proc Natl Acad Sci USA 92:8166, 1995.
34. Toshitani K, Braud V, Browning MJ, Murray N, McMichael AJ, Bodmer WF: Expression of a single-chain HLA class I molecule in a human cell line: Presentation of exogenous peptide and processed antigen to cytotoxic T lymphocytes. Proc Natl Acad Sci USA 93:236, 1996.