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Volumn 273, Issue 5271, 1996, Pages 115-117

Formation of a transition-state analog of the Ras GTPase reaction by Ras·Gdp, tetrafluoroaluminate, and GTPase-activating proteins

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; NEUROFIBROMIN; NEUROMODULIN;

EID: 0030036699     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5271.115     Document Type: Article
Times cited : (194)

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    • We prepared GAP-334 and NF1-333 from E coli as fusion proteins with light meromyosin (LMM) using the expression vector pLMM. Introduction of a protease recognition site for immunoglobulin A protease allowed removal of the LMM part from the proteins of interest
    • We prepared GAP-334 and NF1-333 from E coli as fusion proteins with light meromyosin (LMM) using the expression vector pLMM. Introduction of a protease recognition site for immunoglobulin A protease allowed removal of the LMM part from the proteins of interest.
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    • We thank C. Herrmann for the gift of RBD from c-Raf, P. Stege and D. Vogt for excellent technical help, and R. Schebaum for secretarial assistance
    • We thank C. Herrmann for the gift of RBD from c-Raf, P. Stege and D. Vogt for excellent technical help, and R. Schebaum for secretarial assistance.


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