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We prepared GAP-334 and NF1-333 from E coli as fusion proteins with light meromyosin (LMM) using the expression vector pLMM. Introduction of a protease recognition site for immunoglobulin A protease allowed removal of the LMM part from the proteins of interest
-
We prepared GAP-334 and NF1-333 from E coli as fusion proteins with light meromyosin (LMM) using the expression vector pLMM. Introduction of a protease recognition site for immunoglobulin A protease allowed removal of the LMM part from the proteins of interest.
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47
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9344250157
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We thank C. Herrmann for the gift of RBD from c-Raf, P. Stege and D. Vogt for excellent technical help, and R. Schebaum for secretarial assistance
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We thank C. Herrmann for the gift of RBD from c-Raf, P. Stege and D. Vogt for excellent technical help, and R. Schebaum for secretarial assistance.
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