The fourth EF-hand of calmodulin and its helix-loop-helix components: Impact on calcium binding and enzyme activation

Biochemistry

Volumn 35, Issue 25, 1996, Pages 8307-8313

  George, Samuel E ^a   Su, Zenghua ^a   Fan, Daju ^a   Wang, Songtao ^b   David Johnson, J ^b  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

^b THE OHIO STATE UNIVERSITY WEXNER MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM BINDING PROTEIN; CALCIUM ION; CALMODULIN; MUTANT PROTEIN; MYOSIN LIGHT CHAIN KINASE; NITRIC OXIDE SYNTHASE; PHOSPHODIESTERASE; TROPONIN C;

ARTICLE; BINDING AFFINITY; CALCIUM BINDING; CHIMERA; ENZYME ACTIVATION; ENZYME ASSAY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION;

CALCIUM; CALMODULIN; ENZYME ACTIVATION; HELIX-LOOP-HELIX MOTIFS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MYOSIN-LIGHT-CHAIN KINASE; NITRIC OXIDE SYNTHASE; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; TROPONIN; TROPONIN C;

ESCHERICHIA COLI;

EID: 0030036355     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi960495y     Document Type: Article

References (46)

1. Bagchi, I. C., Huang, Q., & Means, A. R. (1992) Identification of amino acids essential for calmodulin binding and activation of smooth muscle myosin light chain kinase, J. Biol. Chem. 267, 3024-3029.
2. Brandt, P. W., George, S. E., & Schachat, F. (1994) Calmodulin is intrinsically less effective than troponin C in supporting contraction in skinned skeletal muscle fibers. FEBS Lett. 353, 99-102.
3. Bredt, D. S., & Snyder, S. H. (1989) Nitric oxide mediates glutamate-linked enhancement of cGMP levels in the cerebellum, Proc. Natl. Acad. Sci. U.S.A. 86, 9030-9033.
4. Chattopadhyaya, R., Meador, W. E., Means, A. R., & Quiocho, F. A. (1992) Calmodulin structure refined at 1.7 Å resolution, J. Mol. Biol. 228, 1177-1192.
5. 1H NMR spectroscopic studies of calcium-binding proteins. I. Stepwise proteolysis of the C-terminal α-helix of a helix-loop-helix metal-binding domain. Biochemistry 25, 1817-1826.
6. Cox, J. A. (1988) Interactive properties of calmodulin, Biochem. J. 249, 621-629.
7. Crivici, A., & Ikura, M. (1995) Molecular and structural basis of target recognition by calmodulin, Annu. Rev. Biophys. Biomol. Struct. 24, 85-116.
8. Crouch, T. H., & Klee, C. B. (1980) Positive cooperative binding of calcium to bovine brain calmodulin, Biochemistry 19, 3692-3698. P
9. Edman, C. F., George, S. E., Means, A. R., Schulman, H., & Yaswen, P. (1994) Selective activation and inhibition of calmodulin-dependent enzymes by a calmodulin-like protein found in human epithelial cells, Eur. J. Biochem. 226, 725-730.
10. Forsen, S., & Linse, S. (1995) Cooperativity: over the Hill, Trends Biochem. Sci. 20, 495-497.
11. George, S. E., VanBerkum, M. F. A., Ono, T., et al. (1990) Chimeric calmodulin-cardiac troponin C proteins differentially activate calmodulin target enzymes, J. Biol. Chem. 265, 9228-9235.
12. George, S. E., Su, Z., Fan, D., & Means, A. R. (1993) Calmodulin-cardiac troponin C chimeras: effects of domain exchange on calcium binding and enzyme activation, J. Biol. Chem. 268, 25213-25220.
13. Grabarek, Z., Tao, T., Gergely, J. (1992) Molecular mechanism of troponin-C function, J. Muscle Res. Cell Motil. 13, 383-393.
14. Harris, E., Yaswen, P., & Thorner, J. (1995) Gain-of-function mutations in a human calmodulin-like protein identify residues critical for calmodulin action in yeast, Mol. Gen. Genet. 247, 137-147.
15. Herzberg, O., & James, M. N. G. (1988) Refined crystal structure of troponin C from turkey skeletal muscle at 2.0 Å resolution, J. Mol. Biol. 203, 761-779.
16. Holroyde, M. J., Robertson, S. P., Johnson, J. D., Solaro, R. J., & Potter, J. D. (1980) The calcium and magnesium binding sites on cardiac troponin and their role in the regulation of myofibrillar adenosine triphosphatase, J. Biol. Chem. 255, 11688-11693.
17. Horton, R. M., Hunt, H. D., Ho, S. N., Pullen, J. K., & Pease, L. R. (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension, Gene 77, 61-68.
18. Ikura, M., Clore, G. M., Gronenborn, A. M., Zhu, G., Klee, C. B., & Bax, A. (1992) Solution structure of a calmodulin-target peptide complex by multidimensional NMR, Science 256, 632-638.
19. 2+-specific sites of cardiac troponin and troponin C, J. Biol. Chem. 255, 9635-9640.
20. 2+-specific regulatory sites of troponin C, J. Biol. Chem. 269, 8919-8923.
21. Kretsinger, R. H. (1987) Calcium coordination and the calmodulin fold: divergent versus convergent evolution, Cold Spring Harbor Symp. Quant. Biol. 52, 499-510.
22. Leavis, P. C., & Kraft, E. L. (1978) Calcium binding to cardiac troponin C, Arch. Biochem. Biophys. 186, 411-415.
23. Linse, S., Brodin, P., Johansson, C., Thulin, E., Grundstrom, T., & Forsén, S. (1988) The role of protein surface charges in ion binding, Nature 335, 651-652.
24. Linse, S., Helmersson, A., & Forsén, S. (1991) Calcium binding to calmodulin and its globular domains, J. Biol. Chem. 266, 8050-8054.
25. 1H NMR studies of synthetic peptide analogues of calcium-binding site III of rabbit skeletal troponin C: effect on the lanthanum affinity of the interchange of aspartic acid and asparagine residues at the metal ion coordinating positions. Biochemistry 27, 4198-4206.
26. Meador, W. E., Means, A. R., & Quiocho. F. A. (1992) Target enzyme recognition by calmodulin-2.4 Å structure of a calmodulin-peptide complex, Science 257, 1251-1255.
27. Meador, W. E., Means, A. R., & Quiocho, F. A. (1993) Modulation of calmodulin plasticity in molecular recognition on the basis of X-ray structures, Science 262, 1718-1721.
28. Means, A. R., VanBerkum, M. F. A., Bagchi, I., Lu, K. P., & Rasmussen, C. D. (1991) Regulatory functions of calmodulin. Pharmacol. Ther. 50, 255-270.
29. Ohya, Y., & Botstein, D. (1994) Diverse essential functions revealed by complementing yeast calmodulin mutants, Science 263, 963-966.
30. Palmisano, W. A., Trevino, C. L., & Henzl, M. T. (1990) Site-specific replacement of amino acid residues within the CD binding loop of rat oncomodulin, J. Biol. Chem. 265, 14450-14456.
31. Procyshyn, R. M., & Reid, R. E. (1994) A structure/activity study of calcium affinity and selectivity using a synthetic peptide model of the helix-loop-helix calcium-binding motif. J. Biol. Chem. 269, 1641-1647.
32. Reid, R. E. (1990) Synthetic fragments of calmodulin calcium-binding site III. A test of the acid pair hypothesis, J. Biol. Chem. 265, 5971-5976.
33. Reid, R. E., & Hodges, R. S. (1980) Cooperativity and calcium/magnesium binding to troponin C and muscle calcium binding parvalbumin: an hypothesis, J. Theor. Biol. 84, 401-444.
34. Reid, R. E., Gariepy, J., Saund, A. K., & Hodges, R. S. (1981) Calcium-induced protein folding: structure-affinity relationships in synthetic analogues of the helix-loop-helix calcium binding unit, J. Biol. Chem. 256, 2742-2751.
35. Renner, M., Danielson, M. A., & Falke, J. J. (1993) Kinetic control of Ca(II) signaling: Tuning the ion dissociation rates of EF-hand Ca(II) binding sites, Proc. Natl. Acad. Sci. U.S.A. 90, 6493-6497.
36. Sekharudu, Y. C., & Sundaralingam, M. (1988) A structure-function relationship for the calcium affinities of regulatory proteins containing "EF-hand" pairs, Protein Eng. 2, 139-146.
37. 1H NMR studies of calcium binding to synthetic and variant site III helix-loop-helix peptides. Biochemistry 30, 8339-8347.
38. Strynadka, N. C. J., & James, M. N. G. (1989) Crystal structures of the helix-loop-helix calcium binding proteins, Annu. Rev. Biochem. 58, 951-998.
39. Su, Z., Fan, D., & George, S. E. (1994) Role of domain 3 of calmodulin in activation of calmodulin-stimulated phosphodiesterase and smooth muscle myosin light chain kinase, J. Biol. Chem. 269, 16761-16765.
40. Su, Z., Blazing, M. A., Fan, D., & George, S. E. (1995) The calmodulin-nitric oxide synthase interaction: critical role of the calmodulin latch domain in enzyme activation, J. Biol. Chem. 270, 29117-29122.
41. Trigo-Gonzalez, G., Awang, G., Racher, K., Neden, K., & Borgford, T. (1993) Helix variants of troponin C with tailored calcium affinities, Biochemistry 32, 9826-9831.
42. VanBerkum, M. F. A., George, S. E., & Means, A. R. (1990) Calmodulin activation of target enzymes: consequences of deletions in the central helix, J. Biol. Chem. 265, 3750-3756.
43. Vorherr, T., Knöpfel, L., Hofmann, F., Mollner, S., Pfeuffer, T., & Carafoli, E. (1993) The calmodulin binding domain of nitric oxide synthase and adenylyl cyclase, Biochemistry 32, 6081-6088.
44. Walsh, M. P., Vallet, B., Cavadore, J., & Demaille, J. G. (1980) Homologous calcium-binding proteins in the activation of skeletal, cardiac, and smooth muscle myosin light chain kinases. J. Biol. Chem. 255, 335-337.
45. 2+ binding in calmodulin, Biochemistry 32, 7866-7871.
46. Zhang, M., & Vogel, H. J. (1994) Characterization of the calmodulin-binding domain of rat cerebellar nitric oxide synthase, J. Biol. Chem. 269, 981-985.