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Volumn 116, Issue 2, 1996, Pages 233-241

Methods for studying synthesis, turnover, and phosphorylation of catalytic subunit of cAMP-dependent protein kinase in mammalian cells

Author keywords

Blotting, Western; Chemiluminescence; Cyclic AMP dependent protein kinase; Immunoadsorption

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; ENZYME ANTIBODY; METHIONINE; SULFUR 35; TRITIUM;

EID: 0030035036     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/0303-7207(95)03719-5     Document Type: Article
Times cited : (7)

References (29)
  • 1
    • 0023000363 scopus 로고
    • Evidence for a second isoform of the catalytic subunit of cAMP-dependent protein kinase
    • Uhler, M.D., Chrivia, J.C. and McKnight, G.S. (1986) Evidence for a second isoform of the catalytic subunit of cAMP-dependent protein kinase. J. Biol. Chem. 261, 15360-15363.
    • (1986) J. Biol. Chem. , vol.261 , pp. 15360-15363
    • Uhler, M.D.1    Chrivia, J.C.2    McKnight, G.S.3
  • 2
    • 0022894653 scopus 로고
    • A cloned bovine cDNA encodes an alternate form of the catalytic subunit of cAMP-dependent protein kinase
    • Showers, M.O. and Maurer, R.A. (1986) A cloned bovine cDNA encodes an alternate form of the catalytic subunit of cAMP-dependent protein kinase. J. Biol. Chem. 261, 16288-16291.
    • (1986) J. Biol. Chem. , vol.261 , pp. 16288-16291
    • Showers, M.O.1    Maurer, R.A.2
  • 3
    • 0025343605 scopus 로고
    • Molecular cloning of a tissue specific protein kinase (Cγ) from human testis - Representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase
    • Beebe, S.J., Øyen, O., Sandberg, M., Frøysa, A., Hansson, V. and Jahnsen, T. (1990) Molecular cloning of a tissue specific protein kinase (Cγ) from human testis - representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase. Mol. Endocrinol. 4, 465-475.
    • (1990) Mol. Endocrinol. , vol.4 , pp. 465-475
    • Beebe, S.J.1    Øyen, O.2    Sandberg, M.3    Frøysa, A.4    Hansson, V.5    Jahnsen, T.6
  • 4
    • 0024439358 scopus 로고
    • Affinity purification of the Cα and Cβ isoforms of the catalytic subunit of cAMP-dependent protein kinase
    • Olsen, S.R. and Uhler, M.D. (1989) Affinity purification of the Cα and Cβ isoforms of the catalytic subunit of cAMP-dependent protein kinase. J. Biol. Chem. 264, 18662-18666.
    • (1989) J. Biol. Chem. , vol.264 , pp. 18662-18666
    • Olsen, S.R.1    Uhler, M.D.2
  • 5
    • 0025330489 scopus 로고
    • cAMP-dependent protein kinase: A framework for a diverse family of regulatory enzymes
    • Taylor, S.S., Buechler, J.A. and Yonemoto, W. (1990). cAMP-dependent protein kinase: a framework for a diverse family of regulatory enzymes. Annu. Rev. Biochem. 59, 971-1005.
    • (1990) Annu. Rev. Biochem. , vol.59 , pp. 971-1005
    • Taylor, S.S.1    Buechler, J.A.2    Yonemoto, W.3
  • 6
    • 0027199055 scopus 로고
    • Identification of phosphorylation sites in the recombinant catalytic subunit of cAMP-dependent protein kinase
    • Yonemoto, W., Garrod, S.M., Bell, S.M. and Taylor, S.S. (1993) Identification of phosphorylation sites in the recombinant catalytic subunit of cAMP-dependent protein kinase. J. Biol. Chem. 268, 18626-18632.
    • (1993) J. Biol. Chem. , vol.268 , pp. 18626-18632
    • Yonemoto, W.1    Garrod, S.M.2    Bell, S.M.3    Taylor, S.S.4
  • 7
    • 0024310170 scopus 로고
    • A mutation in the catalytic subunit of protein kinase A prevents myristylation but does not inhibit biological activity
    • Clegg, C.H., Ran, W., Uhler, M.D. and McKnight, G.S. (1989) A mutation in the catalytic subunit of protein kinase A prevents myristylation but does not inhibit biological activity. J. Biol. Chem. 264, 20140-20146.
    • (1989) J. Biol. Chem. , vol.264 , pp. 20140-20146
    • Clegg, C.H.1    Ran, W.2    Uhler, M.D.3    McKnight, G.S.4
  • 8
    • 0027407640 scopus 로고
    • Autoactivation of catalytic (Cα) subunit of cyclic AMP-dependent protein kinase by phosphorylation of threonine 197
    • Steinberg, R.A., Cauthron, R.D., Symcox, M.M. and Shuntoh, H. (1993) Autoactivation of catalytic (Cα) subunit of cyclic AMP-dependent protein kinase by phosphorylation of threonine 197. Mol. Cell. Biol. 13, 2332-2341.
    • (1993) Mol. Cell. Biol. , vol.13 , pp. 2332-2341
    • Steinberg, R.A.1    Cauthron, R.D.2    Symcox, M.M.3    Shuntoh, H.4
  • 9
    • 0020358927 scopus 로고
    • Localization of the catalytic subunit of cyclic AMP-dependent protein kinase in cultured cells using a specific antibody
    • Murtaugh, M.P., Steiner, A.L. and Davies, P.J.A. (1982) Localization of the catalytic subunit of cyclic AMP-dependent protein kinase in cultured cells using a specific antibody. J. Cell Biol. 95, 64-72.
    • (1982) J. Cell Biol. , vol.95 , pp. 64-72
    • Murtaugh, M.P.1    Steiner, A.L.2    Davies, P.J.A.3
  • 10
    • 0021023170 scopus 로고
    • Revenants of a trans-dominant S49 mouse lymphoma mutant that affects expression of cAMP-dependent protein kinase
    • van Daalen Wetters, T., Murtaugh, M.P. and Coffino, P. (1983) Revenants of a trans-dominant S49 mouse lymphoma mutant that affects expression of cAMP-dependent protein kinase. Cell 35, 311-320.
    • (1983) Cell , vol.35 , pp. 311-320
    • Van Daalen Wetters, T.1    Murtaugh, M.P.2    Coffino, P.3
  • 11
    • 0026098765 scopus 로고
    • A kinase-negative mutant of S49 mouse lymphoma cells is defective in posttranslational maturation of catalytic subunit of cyclic AMP-dependent protein kinase
    • Steinberg, R.A. (1991) A kinase-negative mutant of S49 mouse lymphoma cells is defective in posttranslational maturation of catalytic subunit of cyclic AMP-dependent protein kinase. Mol. Cell. Biol. 11, 705-712.
    • (1991) Mol. Cell. Biol. , vol.11 , pp. 705-712
    • Steinberg, R.A.1
  • 12
    • 0016711037 scopus 로고
    • High resolution two-dimensional electrophoresis of proteins
    • O'Farrell, P.H. (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007-4021.
    • (1975) J. Biol. Chem. , vol.250 , pp. 4007-4021
    • O'Farrell, P.H.1
  • 13
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during the assembly of the head of bacteriophage T4
    • Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-685.
    • (1970) Nature (London) , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 14
    • 0023511413 scopus 로고
    • Copper staining: A five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels
    • Lee, C., Levin, A. and Branton, D. (1987) Copper staining: a five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels. Anal. Biochem. 166, 308-312.
    • (1987) Anal. Biochem. , vol.166 , pp. 308-312
    • Lee, C.1    Levin, A.2    Branton, D.3
  • 16
    • 0026408833 scopus 로고
    • Regulation of the human enkephalin promoter by two isoforms of the catalytic subunit of cyclic adenosine 3′,5′-monophosphate-dependent protein kinase
    • Huggenvik, J.I., Collard, M.W., Stofko, R.E., Seasholtz, A.F. and Uhler, M.D. (1991) Regulation of the human enkephalin promoter by two isoforms of the catalytic subunit of cyclic adenosine 3′,5′-monophosphate-dependent protein kinase. Mol. Endocnnol. 5, 921-930.
    • (1991) Mol. Endocnnol. , vol.5 , pp. 921-930
    • Huggenvik, J.I.1    Collard, M.W.2    Stofko, R.E.3    Seasholtz, A.F.4    Uhler, M.D.5
  • 17
    • 0018219457 scopus 로고
    • Kinase-negative mutants of S49 mouse lymphoma cells carry a trans-dominant mutation affecting expression of cAMP-dependent protein kinase
    • Steinberg, R.A., van Daalen Wetters, T. and Coffino, P. (1978) Kinase-negative mutants of S49 mouse lymphoma cells carry a trans-dominant mutation affecting expression of cAMP-dependent protein kinase. Cell 15, 1351-1361.
    • (1978) Cell , vol.15 , pp. 1351-1361
    • Steinberg, R.A.1    Van Daalen Wetters, T.2    Coffino, P.3
  • 19
    • 0026101995 scopus 로고
    • Analysis of the dominance of mutations in cAMP-binding sites of murine type I cAMP-dependent protein kinase in activation of kinase from heterozygous mutant lymphoma cells
    • Shuntoh, H. and Steinberg, R.A. (1991) Analysis of the dominance of mutations in cAMP-binding sites of murine type I cAMP-dependent protein kinase in activation of kinase from heterozygous mutant lymphoma cells. J. Cell. Physiol. 146, 86-93.
    • (1991) J. Cell. Physiol. , vol.146 , pp. 86-93
    • Shuntoh, H.1    Steinberg, R.A.2
  • 20
    • 0020643220 scopus 로고
    • Radiolabeling and detection methods for studying metabolism of regulatory subunit of cAMP-dependent protein kinase I in intact culture cells
    • Steinberg, R.A. (1983) Radiolabeling and detection methods for studying metabolism of regulatory subunit of cAMP-dependent protein kinase I in intact culture cells. Methods Enzymol. 99F, 233-243.
    • (1983) Methods Enzymol. , vol.99 F , pp. 233-243
    • Steinberg, R.A.1
  • 21
  • 22
    • 0019492995 scopus 로고
    • Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins
    • Merril, C.R., Goldman, D., Sedman, S.A. and Ebert, M.H. (1981) Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211, 1437-1438.
    • (1981) Science , vol.211 , pp. 1437-1438
    • Merril, C.R.1    Goldman, D.2    Sedman, S.A.3    Ebert, M.H.4
  • 23
    • 0016203040 scopus 로고
    • A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels
    • Bonner, W.M. and Laskey, R.A. (1974) A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46, 83-88.
    • (1974) Eur. J. Biochem. , vol.46 , pp. 83-88
    • Bonner, W.M.1    Laskey, R.A.2
  • 25
    • 0025863380 scopus 로고
    • Rat Cα catalytic subunit of the cAMP-dependent protein kinase: cDNA sequence and evidence that it is the only isoform expressed in myoblasts
    • Wiemann, S., Voß, H., Kinzel, V. and Pyerin, W. (1991) Rat Cα catalytic subunit of the cAMP-dependent protein kinase: cDNA sequence and evidence that it is the only isoform expressed in myoblasts. Biochim. Biophys. Acta 1089, 254-256.
    • (1991) Biochim. Biophys. Acta , vol.1089 , pp. 254-256
    • Wiemann, S.1    Voß, H.2    Kinzel, V.3    Pyerin, W.4
  • 26
    • 0025823122 scopus 로고
    • Decreased catalytic subunit mRNA levels and altered catalytic subunit mRNA structure in a cAMP-resistant Chinese hamster ovary cell line
    • Howard, P., Day, K.H., Kim, K.E., Richardson, J., Thomas, J., Abraham, I., Fleischmann, R.D., Gottesman, M.M. and Maurer, R.A. (1991) Decreased catalytic subunit mRNA levels and altered catalytic subunit mRNA structure in a cAMP-resistant Chinese hamster ovary cell line. J. Biol. Chem. 266, 10189-10195.
    • (1991) J. Biol. Chem. , vol.266 , pp. 10189-10195
    • Howard, P.1    Day, K.H.2    Kim, K.E.3    Richardson, J.4    Thomas, J.5    Abraham, I.6    Fleischmann, R.D.7    Gottesman, M.M.8    Maurer, R.A.9
  • 27
    • 0023714421 scopus 로고
    • A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit Cα
    • Maldonado, F. and Hanks, S.K. (1988) A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit Cα. Nucleic Acids Res. 16, 8189-8190.
    • (1988) Nucleic Acids Res. , vol.16 , pp. 8189-8190
    • Maldonado, F.1    Hanks, S.K.2
  • 28
    • 0026476024 scopus 로고
    • Cloning of the Cα catalytic subunit of the bovine cAMP-dependent protein kinase
    • Wiemann, S., Kinzel, V. and Pyerin, W. (1992) Cloning of the Cα catalytic subunit of the bovine cAMP-dependent protein kinase. Biochim. Biophys. Acta 1171, 93-96.
    • (1992) Biochim. Biophys. Acta , vol.1171 , pp. 93-96
    • Wiemann, S.1    Kinzel, V.2    Pyerin, W.3
  • 29
    • 0026749987 scopus 로고
    • Molecular structure of the Cβ catalytic subunit of rat cAMP-dependent protein kinase and differential expression of Cα and Cβ isoforms in rat tissues and cultured cells
    • Shuntoh, H., Sakamoto, N., Matsuyama, S., Saitoh, M. and Tanaka, C. (1992) Molecular structure of the Cβ catalytic subunit of rat cAMP-dependent protein kinase and differential expression of Cα and Cβ isoforms in rat tisues and cultured cells. Biochim. Biophys. Acta 1131, 175-180.
    • (1992) Biochim. Biophys. Acta , vol.1131 , pp. 175-180
    • Shuntoh, H.1    Sakamoto, N.2    Matsuyama, S.3    Saitoh, M.4    Tanaka, C.5


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