The human purH. gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase: Cloning, sequencing, expression, purification, kinetic analysis, and domain mapping

Journal of Biological Chemistry

Volumn 271, Issue 4, 1996, Pages 2225-2233

  Rayl, Elizabeth A ^a   Moroson, Barbara A ^a   Beardsley, G Peter ^a,b  

^a YALE UNIVERSITY   (United States)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5 AMINOIMIDAZOLE 4 CARBOXAMIDE RIBONUCLEOTIDE FORMYLTRANSFERASE; AMINO ACID; COMPLEMENTARY DNA; FOLIC ACID; FOLINIC ACID; GENE PRODUCT; HYDROLASE; INOSINE PHOSPHATE CYCLOHYDROLASE; NUCLEOTIDE; POLYPEPTIDE; PURINE; TRANSFERASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BASE PAIRING; CARBOXY TERMINAL SEQUENCE; CLONING; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; FAST PROTEIN LIQUID CHROMATOGRAPHY; GENE; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STEADY STATE;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BINDING SITES; CLONING, MOLECULAR; CONSENSUS SEQUENCE; DNA PRIMERS; DNA, COMPLEMENTARY; FORMYLTETRAHYDROFOLATES; GENE EXPRESSION; HUMANS; HYDROXYMETHYL AND FORMYL TRANSFERASES; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEOTIDE DEAMINASES; PHOSPHORIBOSYLAMINOIMIDAZOLECARBOXAMIDE FORMYLTRANSFERASE; RNA, MESSENGER; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0030034702     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.4.2225     Document Type: Article

References (27)

1. Ebbole, D. J., and Zalkin, H. (1987) J Biol. Chem. 262, 8274-8287
2. Chopra, A. K., Peterson, J. W., and Prasad, R. (1991) Biochim. Biophys. Acta 1090, 351-354
3. Aiba, A, and Mizobuchi, K. (1989) J. Biol. Chem. 264, 21239-21246
4. Ni, L., Guan. K., Zalkin, H , and Dixon, J. E. (1991) Gene (Amst.) 106,197-205
5. Cook. R. J , Lloyd, E. S , and Wagner, C. (1991 ) J. Biol. Chem. 266,4965-4973
6. Innis, M. A., Gelfand, D. H., and Snmsky, J. J (1990) PCR Protocols: A Guide to Methods and Applications, Academic Press, Inc., San Diego, CA
7. Trahey. M., Wong, G., Halenbeck, R., Rubinfeld, B., Martin, G. A., Ladner, M., Long, C. M., Crosier, W. J., Watt, K, Koths, K, and McCormick, F. (1988) Science 242, 1967-1700
8. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) Proc. Natl. Acad. Sci. U.S A. 74, 5463-5467
9. Smith, G. K., Mueller, W. T., Wasserman, G. F., Taylor, W. D., and Benkovic, S J. (1980) Biochemistry 19, 4314-4321
10. Laemmli, U. K. (1970) Nature 227, 680-685
11. Bradford, M. M. (1976) Anal Biochem. 72, 248-254
12. Rabinowitz, J C. (1963) Methods Enzymol. 6, 814-815
13. Rowe, P. B. (1971) Methods Erzymol 18B, 733-735
14. Blakley, R L. (1969) in Frontiers of Biology (Euberger, A. N., and Tatum, E. L., eds) pp. 66-105, North-Holland Publishing Co., Amsterdam
15. Mueller, W. T., and Benkovic, S. J. (1981) Biochemistry 20, 337-344
16. Black, S. L., Black, M. J., and Mangum, J., H. (1978) Anal. Biochem 90, 397-401
17. Shaw, G., and Wilson, D V. (1962) J. Am. Chem. Soc. 2937-2940
18. Burton. K. (1974) in Data for Biochemical Research (Dawson, R. M. C.,Elliott, D. C., Elliott, W. H., and Jones, K. M., eds) pp. 169-179, Oxford University Press, London
19. Higuchi, R., Krummel, B., and Saiki, R. K. (1988) Nucleic Acids Res. 16, 7351-7367
20. Allegra, C J., Drake, J C., Jolivet, J., and Chabner, B. A. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 4881-4885
21. Szabados, E., Hindmarsh, E. J , Phillips, L., Duggleby, R. G., and Christopherson, R I. (1994) Biochemistry 33, 14237-14245
22. Inglese, J., Johnson, D. L., Shiau, A., Smith, J. M., and Benkovic, S. J. (1990) Biochemistry 29, 1436-1443
23. Inglese, J., Smith, J. M., and Benkovic, S. J. (1990) Biochemistry 29, 6678-6687
24. Kan, C , Gehring, M. R., Nodes, B. R., Janson, C. A., Almassy, R. J., and Hostomska, Z (1992) J. Protein Chem. 11, 467-473
25. Almassy, R. J., Janson, C. A., Kan, C., and Hostomska, Z. (1992) Proc. Natl. Acad. Sci. U. S. A 89, 6114-6118
26. Chen, P., Sehulze-Gahmen, U., Stura, E A., Inglese, J., Johnson, D. L., Marolewski, A., Benkovic, S J , and Wilson, I. A. (1992) J. Mol. Biol. 237, 283-292
27. Klein, C , Chen, P., Arevalo, J. H., Stura, E. A., Marolewski, A., Warren, M. S., Benkovic, S. J , and Wilson, I. A. (1995) J. Mol. Biol. 249, 153-175