In situ PCR: Pathologist's dream or nightmare?

Journal of Pathology

Volumn 178, Issue 1, 1996, Pages 11-20

  O'Leary, J J ^a   Chetty, R ^a   Graham, A K ^a   McGee, J O'D ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

HUMAN; HUMAN TISSUE; IN SITU HYBRIDIZATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVIEW; TECHNIQUE;

DNA, VIRAL; HUMANS; IN SITU HYBRIDIZATION; INDICATORS AND REAGENTS; POLYMERASE CHAIN REACTION; TISSUE FIXATION;

EID: 0030034013     PISSN: 00223417     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-9896(199601)178:1<11::AID-PATH459>3.0.CO;2-R     Document Type: Review

References (22)

1. Herrington CS, de Angelis M, Evans MF, Troncone O, McGee JO'D. Detection of high risk human papilloma virus in routine cervical smears: strategy for screening. J Clin Pathol 1992, 45: 385-390.
2. Haase AT, Retzel EF, Staskus KA Amplification and detection of Ientiviral DNA inside cells. Proc Natl Acad Sci USA 1990; 87: 4971-4975.
3. Nuovo GJ. PCR in-situ Hybridisation. Protocols and Applications. New York: Raven Press, 1992
4. Nuovo GJ, Gallery F, MacConnell P, Becker P, Bloch W An improved technique for the detection of DNA by in-situ hybridisation after PCR amplification. Am J Pathol 1991; 139: 1239-1244
5. Nuovo GJ, MacConnell P, Forde A, Delvenne P. Detection of human papilloma virus DNA in formalin fixed tissues by in-situ hybridisation after amplification by PCR Am J Pathol 1991, 139: 847-850
6. O'Leary JJ, Browne G, Landers RJ, el al. The importance of fixation procedures on DNA template and its suitability for solution phase polymerase chain reaction and PCR in-situ hybridisation. Histochem J 1994; 26: 337-346.
7. Embretson J, Zupancic M, Beneke J, et al. Analysis of human immunodeficiency virus-infected tissues by amplification and in situ hybridisation reveals latent and permissive infection at single cell resolution. Proc Natl Acad Sci USA 1993, 90: 357-361
8. Bagasra O, Hauptman SP, Lischner HW, Sachs M, Pomerantz RJ. Detection of human immunodeficiency virus type 1 provirus in mononuclear cells by in situ polymerase chain reaction. N Engl J Med 1992, 326: 1385-1391.
9. Chiu K, Cohen SH, Morris DW, Jordan GW Intracellular amplification of proviral DNA in tissue sections using the polymerase chain reaction J Histochem Cytochem 1992; 40: 333-341.
10. Komminoth P, Long AA, Ray R, Wolfe HJ In-situ polymerase chain reaction detection of viral DNA single copy genes and gene re-arrangements in cell suspensions and cytospins. Diagn Mol Palhol 1992; 1: 85-97
11. Long AA, Komminoth P, Wolfe HF. Comparison of indirect and direct in-situ polymerase chain reaction in cell preparations and tissue sections Detection of viral DNA and gene re-arrangements and chromosomal translocations. Histochemtstry 1993; 99: 151-162
12. O'Leary JJ, Browne G, Johnson MI, et al PCR m-situ hybridisation detection of HPV 16 in fixed CaSki and fixed SiHa cells-an experimental model system. J Clin Pathol 1994; 47: 933-938.
13. Spann W, Pachmann K. Zabnienska H, Pielmeier A, Emmerich B. In-situ amplification of single copy gene segments in individual cells by the polymerase chain reaction. Infection 1991; 19: 242-244
14. Embleton MJ, Gorochov G, Jones PT, Winter G In cell PCR from mRNA-amplifymg and linking the immunoglobulin heavy and light V genes within single cells. Nucleic Acid Res 1992; 20: 3831-3837.
15. Ray R, Komminoth P, Macado M, Wolfe HJ. Combined polymerase chain reaction and in-situ hybridisation for the detection of single copy genes and viral genome sequences in intact cells. Mod Pathol 1991; 4: 124A.
16. Nuovo GJ, Gallery F, Horn R, MacConnell P, Bloch W. Importance of different variables for enhancing in-situ detection of PCR-amplified DNA. PCR Methods Applications 1993, 2: 305-312.
17. Hopwood D. Cell and tissue fixation, 1972-1982 Hitochem J 1985, 17: 389-442.
18. Staskus KA, Couch L, Bitterman P, et al. In-situ amplification of visna virus DNA in tissue sections reveals a reservoir of latently infected cells. Microbiol Pathol 1991; 11: 67-76.
19. Yap EPH, McGee JO'D. Slide PCR: DNA amplification from cell samples on microscopic glass slides. Nucleic Acid Res 1991, 19: 4294.
20. Gosden J, Hanratty D, Starling J, Fnates J, Mitchell A, Porteous D. Oligonucleotide primed in situ DNA synthesis (PRINS): a method for chromosome mapping, banding and investigation of sequence organisation. Cytogenet Cell Genet 1991; 57: 100-104.
21. Murray GI. In-situ PCR. J Pathol 1993; 169: 187 188.
22. O'Leary JJ, Browne G, Landers RJ, et al. New nucleic acid amplification techniques in diagnostic pathology. J Eur Acad Dermatol Venerol 1994; 3: 78-86.