Conversion of the covalent intermediate 3-fluoro-2-phospho-lactyl-EPTase to 3-fluoro-2-phospholactyl-UDP-GlcNAc

Bioorganic and Medicinal Chemistry Letters

Volumn 6, Issue 2, 1996, Pages 133-138

  Salleh, Hamzah Mohd ^a   Dotson, Garry D ^a   Woodard, Ronald W ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MURAMIC ACID DERIVATIVE; PEPTIDOGLYCAN; TRANSFERASE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE SUGAR;

ARTICLE; DRUG SYNTHESIS; ENZYME ACTIVITY; NUCLEAR MAGNETIC RESONANCE; REACTION ANALYSIS;

EID: 0030033362     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0960-894X(95)00570-J     Document Type: Article

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19. 19F-NMR of the incubation of 3-FPEP with UDP-GalNAc in the presence of our EPTase C115A mutant. If indeed, the enzyme-bound fluorinated-intermediates observed in Figures 1a and c are not present in this new experiment then they must both have been bound to C115. Alternatively, if a peak with a chemical shift identical to that observed in Figure 1c is seen, then a different nucleophile must be labeled. Should the latter be the case, proteolytic digestion coupled with HPLC-MS will be used in order to determine the labeled amino acid residue.