Characterization of human plasma glycoproteins separated by two-dimensional gel electrophoresis

Bio/Technology

Volumn 14, Issue 1, 1996, Pages 66-70

  Packer, Nicolle H ^a   Wilkins, Marc R ^a   Golaz, Olivier ^b   Lawson, Margaret A ^a   Gooley, Andrew A ^a   Hochstrasser, Denis F ^b   Redmond, John W ^c   Williams, Keith L ^a  

^a MACQUARIE UNIVERSITY   (Australia)

^b UNIVERSITY OF GENEVA   (Switzerland)

^c AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOPROTEIN; PLASMA PROTEIN; ALPHA 1 ANTITRYPSIN; AMINO ACID; AMINOSUGAR; FETUIN; MONOSACCHARIDE;

PLASMA; TWO DIMENSIONAL GEL ELECTROPHORESIS; AMINO ACID ANALYSIS; ARTICLE; CATTLE; GLYCOSYLATION; HUMAN; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN PURIFICATION;

ALPHA 1-ANTITRYPSIN; ALPHA-FETOPROTEINS; ANIMALS; CATTLE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; MONOSACCHARIDES; OLIGOSACCHARIDES;

BOS TAURUS; BOVINAE;

EID: 0030033101     PISSN: 0733222X     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0196-66     Document Type: Article

References (29)

1. Wasinger, V. C, Cordwell, S. J., Poljak, A., Yan, J. X., Gooley, A. A., Wilkins, M. R., Duncan, M., Harris, R., Williams, K. L. and Humphery-Smith, I. 1995. Progress with gene-product mapping of the Mollicutes: Mycoplasma genital-ium. Electrophoresis 16: In press.
2. Wilkins, M. R., Sanchez, J.-G, Gooley, A. A., Appel, R. D., Humphery-Smith, I., Hochstrasser, D. F. and Williams, K. L. 1995. Progress with proteome projects: why all proteins expressed by a genome should be identifìed and how to do it. Biotechnology and Genetic Engineering Reviews 13:In press.
3. Golaz, O., Hughes, G. J., Frutiger, S., Paquet, N., Bairoch, A., Pasquali, C., Sanchez, J. C., Tissot, J. D., Appel, R. D., Walzer, C, Balant, L. and Hochstrasser, D. F. 1993. Plasma and red blood cell protein maps: update 1993. Electrophoresis 14:1223–1231.
4. Garrels, J. I., Futcher, B., Kobayashi, R., Latter, I., Schwender, B., Volpe, T., Warner, J. R. and McLaughlin, C. S. 1994. Protein identification for a Saccharomyces cerevisiae protein database. Electrophoresis 15:1466–1486.
5. Latham, K. E., Garrels, J. I., Chang, C. and Solter, D. 1991. Quantitative analysis of protein synthesis in mouse embryos I: extensive re-programming at the one- and two-cell stages. Development 2:921–932.
6. Thomas, S. and Singh, R. S. 1992. A comprehensive study of genie variation in natural populations of Drosophila melanogasler. VII. Varying rates of genie divergence as revealed by two-dimensional electrophoresis. Mol. Biol. Evol. 9:507–525.
7. Garrels, J. I. 1989. The QUEST system for quantitative analysis of two-dimensional gels. J. Biol. Chem. 264:5269–5282.
8. Appel, R. D., Hochstrasser, D. F., Funk, M., Vargas, J. R., Pelligrini, C, Muller, A. F. and Scherrer, J. R. 1991. The MELANIE project: from a biopsy to automatic protein map interpretation by computer. Electrophoresis 12:722–735.
9. Ohno, K., Yuasa, I., Akaboshi, S., Itoh, M., Yoshida, K„ Ehara, H, Ochiai, Y. and Takeshita, K. 1992. The carbohydrate deficient glycoprotein syndrome in three Japanese children. Brain Dev. 14:30–35.
10. Yamishita, K., Ideo, H., Ohkura, T., Fukushima, K., Yuasa, I., Ohno, K. and Takeshita, K. 1993. Sugar chains of serum transferrin from patients with carbohydrate deficient glycoprotein syndrome. Evidence of asparagine N-linked oligosaccharide transfer deficiency. J. Biol. Chem. 268:5783–5789.
11. Bjellqvist, B., Sanchez, J.-C, Pasquali, C, Ravier, F, Paquet, N., Frutiger, S., Hughes, G. J. and Hochstrasser, D. F. 1993. Micropreparative 2-D electrophoresis allowing the separation of milligram amounts of proteins. Electrophoresis 14:1375–1378.
12. Shaw, G. 1993. Rapid identification of proteins. Proc. Natl. Acad. Sci. USA 90:5138–5142.
13. Hobohm, U., Houthaeve, T. and Sander, C. 1994. Amino acid analysis and protein database compositional search as a rapid and inexpensive method to identify proteins. Anal. Biochem. 222:202–209.
14. Wilkins, M. R., Pasquali, C, Appel, R. D., Ou, K., Golaz, O, Sanchez, J.-C, Yan, J. X., Gooley, A. A., Hughes, G., Humphrey-Smith, I., Williams, K. L. and Hochstrasser, D. F. 1995. From proteins to proteosones: large scale protein identification by two-dimensional electrophoresis and amino acid analysis. Bio/Technology 14:61–65.
15. Ogawa, H, Ueno, M., Uchibori, H., Matsumoto, I. and Seno, N. 1990. Direct carbohydrate analysis of glycoproteins electroblotted onto polyvinylidene difluoride membrane from sodium dodecyl sulfate-polyacrylamide gels. Anal. Biochem. 190:165–169.
16. Weitzhandler, M., Kadlecek, D., Avdalovic, N., Forte, J. G., Chow, D. and Townsend, R. R. 1993. Monosaccharide and oligosaccharide analysis of proteins transferred to polyvinylidene fluoride membranes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 268:5121–5130.
17. Spiro, R. G. and Bhoyroo, V. D. 1974. Structure of the O-glycosidically linked carbohydrate units of fetuin. J. Biol. Chem. 249:5704–5717.
18. Townsend, R. R., Hardy, M. R., Cumming, D. A., Carver, J. P. and Bendiak, B. 1989. Separation of branched sialylated oligosaccharides using high-pH anion-exchange chromatography with pulsed amperometric detection. Anal. Biochem. 182:1–8.
19. Hayase, T., Rice, K. G., Dziegielewska, K. M., Kuhlenschmidt, M., Reilly, T. and Lee, Y. C. 1992. Comparison of N-glycosides of fetuins from different species and human alpha 2-HS-glycoprotein. Biochemistry 31:4915–4921.
20. Fan, J.-Q., Namiki, Y., Matsuoka, K. and Lee, Y. C. 1994. Comparison of acid hydrolytic conditions for Asn-linked oligosaccharides. Anal. Biochem. 219:375–378.
21. Devine, P. L. and Warren, J. A. 1990. Glycoprotein detection on immobilon PVDF membrane using the periodic acid/Schiff reagent. BioTechniques 8:492–495.
22. Gravel, P., Golaz, O., Walzer, C, Hochstrasser, D. F., Turler, H. and Balant, L. P. 1994. Analysis of glycoproteins separated by two-dimensional gel electrophoresis using lectin blotting revealed by chemiluminescence. Anal. Biochem. 221:66–71.
23. Anderseon, D. C, Goochee, C. F, Cooper, G. and Weitzhandler, M. 1994. Monosaccharide anhd oligosaccharide analysis of isoelectric focusing-separated and blotted granulocyte colony-stimulating factor glycoforms using high-pH anion-exchange chromatography with pulsed amperometric detection. Glycobiology 4:459–467.
24. Riebe, D. and Thorn, W. 1991. Influence of carbohydrate moieties of human serum transferrin on the determination of its molecular mass by Polyacrylamide gradient gel electrophoresis and staining with periodic acid-Schiff reagent. Electrophoresis 12:287–293.
25. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685.
26. Jin, Y. and Cerletti, N. 1992. Western blotting of transforming growth factor beta-2: optimisation of the electrophoretic transfer. Appl. Theor. Electrophor. 3:85–90.
27. Hochstrasser, A. C, James, R. W., Pometta, D. and Hochstrasser, D. F. 1991. Preparative isoelectrofocusing and high resolution two-dimensional electrophoresis for concentration and purification of proteins. Appl. Theor. Electrophor. 1:333–337.
28. Haynes, P. A., Sheumack, D., Greig, L. G., Kibby, J. and Redmond, J. W. 1991. Applications of automated amino acid analysis using 9-fluorenylmethyl chloroformate. J. Chromatogr. 588:107–114.
29. Appel, R. D., Bairoch, A. and Hochstrasser, D. F. 1994. A new generation of information retrieval tools for biologists: the example of the ExPASy WWW server. Trends in Biochem. Sci. 19:258–260.