Method to measure apolipoprotein B-48 and B-100 secretion rates in an individual mouse: Evidence for a very rapid turnover of VLDL and preferential removal of B-48- Relative to B-100-containing lipoproteins

Journal of Lipid Research

Volumn 37, Issue 1, 1996, Pages 210-220

  Li, Xiaohua ^a   Catalina, Fernando ^b   Grundy, Scott M ^a,b   Patel, Shailesh ^a,b  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Gel electrophoresis; Hepatic secretion; Laser densitometry; mRNA editing; Triton

Indexed keywords

APOLIPOPROTEIN B100; APOLIPOPROTEIN B48; VERY LOW DENSITY LIPOPROTEIN;

ANIMAL EXPERIMENT; ARTICLE; FEMALE; LIPOPROTEIN METABOLISM; LIVER METABOLISM; MALE; MOUSE; NONHUMAN; PLASMA CLEARANCE; PRIORITY JOURNAL; PROTEIN SECRETION;

ANIMALS; APOLIPOPROTEIN B-100; APOLIPOPROTEIN B-48; APOLIPOPROTEINS B; BASE SEQUENCE; DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; LIPOPROTEINS, VLDL; LIVER; MALE; METABOLIC CLEARANCE RATE; MICE; MICE, INBRED C57BL; MOLECULAR SEQUENCE DATA; PHYSIOLOGY; POLYETHYLENE GLYCOLS; SEX CHARACTERISTICS; TRIGLYCERIDES;

EID: 0030032986     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (39)

1. Havel, R. J., and J. P. Kane. 1989. Disorders of the biogenesis and secretion of lipoproteins containing the B lipoproteins. In The Metabolic Basis of Inherited Disease. C. R. Scriver, A. L. Beaudet, W. S. Sly, and D. Valle, editors. McGraw-Hill Inc., New York. 1129-1164.
2. Greeve, J., I. Altkemper, J. H. Dieterich, H. Greten, and E. Windler. 1993. Apolipoprotein B mRNA editing in 12 different mammalian species: hepatic expression is reflected in low concentrations of apoB-containing plasma lipoproteins. J. Lipid Res. 34: 1367-1383.
3. Grundy, S. M., and G. L. Vega. 1990. What is meant by overproduction of apoB-containing lipoproteins? In Hypercholesterolemia, Hypocholesterolemia, Hypertriglyceridemia, In Vivo Kinetics. C. L. Malmendier, P. Alaupovic, and H. B. Brewer, Jr., editors. Plenum Press, New York and London. 213-222.
4. Kellner, A., J. W. Correll, and A. T. Ladd. 1951. Sustained hyperlipemia induced in rabbits by means of intravenously injected surface active agents. J. Exp. Med. 93: 373-385.
5. Scanu, A., P. Oriente, J. M. Szajewski, L. J. McCormack, and I. H. Page. 1961. Triton hyperlipemia in dogs. II. Atherosclerosis, diffuse lipidosis and depletion of fat stores produced by prolonged administration of the non-ionic surface-active agent. J. Exp. Med. 114: 279-294.
6. Scanu, A., and P. Oriente. 1961. Triton hyperlipemia in dogs. I. In vitro effects of the detergent on serum lipoproteins and chylomicrons. J. Exp. Med. 113: 735-757.
7. Scanu, A. 1965. Factors affecting lipoprotein metabolism. Adv. Lipid Res. 3: 63-138.
8. Recknagel, R. O. 1967. Carbon tetrachloride hepatotoxicity. [Review]. Pharmacol. Rev. 19: 145-208.
9. Otway, S., and D. S. Robinson. 1967. The use of a non-ionic detergent (Triton WR 1339) to determine rates of triglyceride entry into the circulation of the rat under different physiological conditions. J. Physiol. 190: 321-332.
10. Otway, S., and D. S. Robinson. 1967. The effect of a non-ionic detergent (Triton WR 1339) on the removal of triglyceride fatty acids from the blood of the rat. J. Physiol. 190: 309-319.
11. Zilversmit, D. B., L. B. Hughes, and M. Remington. 1972. Hypolipidemic effect of pregnancy in the rabbit. J. Lipid Res. 13: 750-756.
12. Schurr, P. E., J. R. Schultz, and T. M. Parkinson. 1972. Triton-induced hyperlipidemia in rats as an animal model for screening hypolipidemic drugs. Lipids. 7: 68-74.
13. Klauda, H. C., and D. B. Zilversmit. 1974. Influx of cholesterol into plasma in rabbits with fasting hyperbetalipoproteinemia. J. Lipid Res. 15: 593-601.
14. Eisenberg, S., and R. I. Levy. 1975. Lipoprotein metabolism. [Review]. Adv. Lipid Res. 13: 1-89.
15. Illingworth, D. R., L. E. Whipple, and O. W. Portman. 1975. Metabolism of lipoproteins in nonhuman primates. Reduced secretion of very low density lipoproteins in squirrel monkeys with diet-induced hypercholesterolemia. Atherosclerosis. 22:325-334.
16. Illingworth, D. R. 1975. Metabolism of lipoproteins in nonhuman primates. Studies on the origin of low density lipoprotein apoprotein in the plasma of the squirrel monkey. Biochim. Biophys. Acta. 388: 38-51.
17. Borensztajn, J., M. S. Rone, and T. J. Kotlar. 1976. The inhibition in vivo of lipoprotein lipase (clearing-factor lipase) activity by Triton WR-1339. Biochem. J. 156: 539-543.
18. Portman, O. W., M. Alexander, N. Tanaka, and D. R. Illingworth. 1977. Triacylglycerol and very low density lipoprotein secretion into plasma of squirrel monkeys. Biochim. Biophys. Acta. 486: 470-482.
19. Ishikawa, T., and N. Fidge. 1979. Changes in the concentration of plasma lipoproteins and apoproteins following the administration of Triton WR 1339 to rats. J. Lipid Res. 20: 254-264.
20. Karpe, F., G. Steiner, T. Olivecrona, L. A. Carlson, and A. Hamsten. 1993. Metabolism of triglyceride-rich lipoproteins during alimentary lipemia. J. Clin. Invest. 91: 748-758.
21. Karpe, F., and A. Hamsten. 1994. Determination of apolipoproteins B-48 and B-100 in triglyceride-rich lipoproteins by analytical SDS-PAGE. J. Lipid Res. 35: 1311-1317.
22. Kotite, L., N. Bergeron, and R. J. Havel. 1995. Quantification of apolipoproteins B-100, B-48, and E in human triglyceride-rich lipoproteins. J. Lipid Res. 36: 890-900.
23. LeBoeuf, R. C., M. Caldwell, and E. Kirk. 1994. Regulation by nutritional status of lipids and apolipoproteins A-I, A-II, and A-IV in inbred mice. J. Lipid Res. 35: 121-133.
24. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685.
25. Blackhart, B. D., E. M. Ludwig, V. R. Pierotti, L. Caiati, M. A. Onasch, S. C. Wallis, L. Powell, R. Pease, T. J. Knott, M. L. Chu, R. W. Mahley, J. Scott, B. J. McCarthy, and B. Levy-Wilson. 1986. Structure of the human apolipoprotein B gene. J. Biol. Chem. 261: 15364-15367.
26. Rasband, W. 1994. NIH Image. Available via Internet. ftp from zippy.nihm.nih.gov, or on floppy disk from NTIS, 5285 Port Royal Rd., Springfield, VA 22161, part number PB93-504868.
27. Jialal, L, C. J. Fuller, and B. A. Huet. 1995. The effect of α-tocopherol supplementation on LDL oxidation. A dose-response study. Arterioscler. Thromb. Vasc. Biol. 15: 190-198.
28. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
29. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159.
30. Chomczynski, P. 1993. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques. 15: 532-534.
31. Patel, S., M. Pessah, I. Beucler, J. Navarro, and R. Infante. 1994. Chylomicron retention disease: exclusion of apolipoprotein B gene defects and detection of mRNA editing in an affected family. Atherosclerosis. 108: 201-207.
32. Abdel-Fattah, G., M. L. Fernandez, and D. J. McNamara. 1995. Regulation of guinea pig very low density lipoprotein secretion rates by dietary fat saturation. J. Lipid Res. 36: 1188-1198.
33. de Silva, H. V., J. Mas-Oliva, J. M. Taylor, and R. W. Mahley. 1994. Identification of apolipoprotein B-100 low density lipoproteins, apolipoprotein B48 remnants, and apolipoprotein E-rich high density lipoproteins in the mouse. J. Lipid Res. 35: 1297-1310.
34. Hayashi, H., K. Fujimoto, J. A. Cardelli, D. F. Nutting, S. Bergstedt, and P. Tso. 1990. Fat feeding increases size, but not number, of chylomicrons produced by small intestine. Am. J. Physiol. 259: G709-G719.
35. Kesäniemi, Y. A., G. L. Vega, and S. M. Grundy. 1992. Kinetics of apolipoprotein B in normal and hyperlipidemic man: review of current data. In Lipoprotein Kinetics and Modelling. M. Berman, S. M. Grundy, and B. V. Howard, editors. Academic Press, New York, 181-205.
36. Grundy, S. M., H. Y. Mok, L. Zech, D. Steinberg, and M. Berman. 1979. Transport of very low density lipoprotein triglycerides in varying degrees of obesity and hypertriglyceridemia. J. Clin. Invest. 63: 1274-1283.
37. Eaton, R. P., R. C. Allen, and D. S. Schade. 1983. Over-production of a kinetic subclass of VLDL-apoB, and direct catabolism of VLDL-apoB in human endogenous hypertriglyceridemia: an analytical model solution of tracer data. J. Lipid Res. 24: 1291-1303.
38. Huff, M. W., P. M. Giovannetti, and B. M. Wolfe. 1984. Turnover of very low-density lipoprotein-apoprotein B is increased by substitution of soybean protein for meat and dairy protein in the diets of hypercholesterolemic men. Am. J. Clin. Nutr. 39: 888-897.
39. Cryer, D. R., T. Matsushima, J. B. Marsh, M. Yudkoff, P. M. Coates, and J. A. Cortner. 1986. Direct measurement of apolipoprotein B synthesis in human very low density lipoprotein using stable isotopes and mass spectrometry, J. Lipid Res. 27: 508-516.