Inorganic, monovalent cations compete with agonists for the transmitter binding site of nicotinic acetylcholine receptors

Biophysical Journal

Volumn 70, Issue 6, 1996, Pages 2652-2658

  Akk, Gustav ^a   Auerbach, Anthony ^a  

^a UNIVERSITY AT BUFFALO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINE; CESIUM ION; NICOTINIC RECEPTOR; POTASSIUM ION; SODIUM ION; TETRAMETHYLAMMONIUM;

ARTICLE; BINDING SITE; CHANNEL GATING; CHOLINERGIC SYNAPSE; DISSOCIATION CONSTANT; MATHEMATICAL ANALYSIS; MOUSE; NEUROTRANSMISSION; NONHUMAN; POTASSIUM CURRENT; SLOW INWARD CURRENT; SODIUM CURRENT; TRANSPORT KINETICS;

EID: 0030032121     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(96)79834-X     Document Type: Article

References (30)

1. Adams, D. J., T. M. Dwyer, and B. Hille. 1980. The permeability of endplate channels to monovalent and divalent metal cations. J. Gen. Physiol. 75:493-510.
2. Auerbach, A. 1993. A statistical analysis of acetylcholine receptor activation in Xenopus myocytes: stepwise vs concerted models of gating. J. Physiol. (Lond.). 461:339-378.
3. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1992. Short Protocols in Molecular Biology. John Wiley and Sons, New York.
4. Aylwin, M. L., and M. M. White. 1994. Gating properties of mutant acetylcholine receptors. Mol. Pharmacol. 46:1149-1155.
5. Changeaux, J.-P., J.-L. Galzi, A. Devillers-Thierry, and D. Bertrand. 1992. The functional architecture of the acetylcholine nicotinic receptor explored by affinity labeling and site-directed mutagenesis. Q. Rev. Biophys. 25:395-432.
6. Chen, J., Y. Zhang, G. Akk, S. Sine, and A. Auerbach. 1995. Activation kinetics of recombinant mouse nicotinic acetylcholine receptors: mutations of α-subunit tyrosine 190 affect both binding and gating. Biophys. J. 69:849-859.
7. Colquhoun, D., and A. G. Hawkes. 1977. Relaxation and fluctuations of membrane currents that flow through drug-operated channels. Proc. R. Soc. Lond. B. 199:231-262.
8. Colquhoun, D., and F. J. Sigworth. 1983. Fitting and statistical analysis of single-channel records. In Single-Channel Recording. B. Sakmann and E. Neher, editors. 191-264.
9. del Castillo, J., and Katz, B. 1957. Interaction at endplate receptors between different choline derivatives. Proc. R. Soc. Lond. B. 146:369-381.
10. Gage, P. W., and D. Van Helden. 1979. Effects of permeant monovalent cations on end-plate channels. J. Physiol. (Lond.). 288:509-528.
11. Gibson, R. E., S. Juni, and R. O'Brien. 1977. Monovalent ion effects on acetylcholine receptor from Torpedo californica. Arch. Biochem. Biophys. 179:183-188.
12. Hamill, O. P., A. Many, E. Neher, B. Sakmann, and F. J. Sigworth. 1981. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 391:85-100.
13. Hille, B. 1992. Ionic Channels of Excitable Membranes. Sinauer Associates, Sunderland, MA.
14. Hirano, T., Y. Kidokoro, and H. Ohmori. 1987. Acetylcholine doseresponse relation and the effect of cesium ions in the rate adrenal chromaffin cell under voltage clamp. Pflugers Arch. 408:401-407.
15. Horn, R., and K. Lange. 1993. Estimating kinetic constants from single channel data. Biophys. J. 43:207-223.
16. Magleby, K. L., and C. F. Stevens. 1972. A quantitative description of end-plate currents. J. Physiol (Lond.). 223:173-197.
17. Marshall, C. G., D. Ogden, and D. Colquhoun. 1991. Activation of ion channels in the frog endplate by several analogues of acetylcholine. J. Physiol (Lond.). 433:73-93.
18. O'Leary, M. E., and M. M. White. 1992. Mutational analysis of ligand-induced activation of the Torpedo acetylcholine receptor. J. Biol. Chem. 267:8360-8365.
19. Qin, F., A. Auerbach, and F. Sachs. 1996. Estimating single-channel kinetic parameters from idealized patch-clamp data containing missed events. Biophys. J. 70:264-280.
20. Roux, B., and R. Sauve. 1985. A general solution to the time interval omission problem applied to single channel analysis. Biophys. J. 48: 149-158.
21. Sakmann, B., J. Patlak, and E. Neher. 1980. Single acetylcholine-activated channels show burst-kinetics in presence of desensitizing concentrations of agonist. Nature. 286:71-73.
22. Sine, S. M. 1993. Molecular dissection of subunit interfaces in the acetylcholine receptor: identification of residues that determine curare selectivity. Proc. Natl. Acad. Sci USA. 90:9436-9440.
23. Sine, S. M., T. Claudio, and F. J. Sigworth. 1990. Activation of Torpedo acetylcholine receptors expressed in mouse fibroblasts. J. Gen. Physiol. 96:395-437.
24. Sine, S., K. Ohno, C. Bouzat, A. Auerbach, M. Milone, J. N. Pruitt, and A. Engel. 1995. Mutation of the acetylcholine receptor α-subunit causes a congenital myasthenic syndrome by enhancing agonist binding affinity. Neuron. 15:229-239.
25. Sine, S. M., P. Quiram, F. Papanikolaou, H.-J. Kreienkamp, and P. Taylor. 1994. Conserved tyrosines in the alpha subunit of the nicotinic acetylcholine receptor stabilize quaternary ammonium groups of agonists and curariform antagonists. J. Biol. Chem. 269:8808-8816.
26. Smith, S. M., and McBurney, R. N. 1989. Caesium ions: a glycineactivated channel agonist in rat spinal cord neurones grown in cell culture. Br. J. Pharmacol. 96:940-948.
27. Sussman, J. L., M. Harel, F. Frolow, C. Oefner, A. Goldman, L. Toker, and I. Silman. 1991. Atomic structure of acetylcholinesterase from Torpedo californica: a prototypic acetylcholine-binding protein. Science. 253: 872-879.
28. Tomaselli, G. F., J. T. McLaughlin, M. E. Jurman, E. Hawrot, and G. Yellen. 1991. Mutations affecting agonist sensitivity of the nicotinic acetylcholine receptor. Biophys. J. 60:721-727.
29. Unwin, N. 1993. Nicotinic acetylcholine receptor at 9 Å resolution. J. Mol. Biol. 229:1101-1124.
30. Zhang, Y., J. Chen, and A. Auerbach. 1995. Kinetics of wild-type, embryonic nicotinic acetylcholine receptors activated by acetylcholine, carbamylcholine, and tetramethylammonium. J. Physiol. (Lond.). 481: 189-206.