Down regulation of S-adenosyl-L-methionine decarboxylase activity of Trypanosoma brucei during transition from long slender to short stumpy-like forms in axenic culture

European Journal of Cell Biology

Volumn 69, Issue 2, 1996, Pages 173-179

  Selzer, Paul Maria ^a,b   Hesse, Friedemann ^a   Hamm Künzelmann, Brigitte ^a,c   Mühlstädt, Kerstin ^a   Echner, Hartmut ^a   Duszenko, Michael ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

Axenic cultivation; Ornithine decarboxylase; S adenosyl L methionine decarboxylase; Trypanosoma brucei

Indexed keywords

ADENOSYLMETHIONINE DECARBOXYLASE; CELL PROTEIN; ORNITHINE; ORNITHINE DECARBOXYLASE; POLYAMINE; PUTRESCINE;

ARTICLE; CELL DIFFERENTIATION; CELL DIVISION; CONTROLLED STUDY; CULTURE MEDIUM; DOWN REGULATION; ENZYME ACTIVITY; NONHUMAN; PARASITE CULTIVATION; PARASITOSIS; POLYAMINE METABOLISM; PRIORITY JOURNAL; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0030031834     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Bacchi, C. J., J. Garofalo, D. Mockenhaupt, P. P. McCann, K. A. Diekema, A. E. Pegg, H. C. Nathan, E. A. Mullaney, L. Chunosoff, A. Sjoerdsma, S. H. Hunter: In vivo effect of α-DL-difluoromethylornithine on the metabolism and morphology of Trypanosoma brucei brucei. Mol Biochem. Parasitol. 7, 209-225 (1983).
2. Bacchi, C. J., P. P. McCann: Parasitic protozoa and polyamines. In: P. P. McCann, A. E. Pegg, A. Sjoerdsma (eds.): Inhibition of Polyamine Metabolism. Biological significance and basis for new therapies. pp. 317-344. Academic Press. New York 1987.
3. Bacchi, C. J., J. Garofalo, A. Santana, J. C. Hannan, A. J. Bitonti, P. P. McCann: Trypanosoma brucei brucei: Regulation of ornithine decarboxylase in procyclic forms and trypomastigotes. Exp. Parasitol. 68, 392-402 (1989).
4. Baltz, T., D. Baltz, C. B. Giroud, J. Crockett: Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense. EMBO J. 4, 1273-1277 (1985).
5. Bass, K. E., C. C. Wang: The in vitro differentiation of pleomorphic Trypanosoma brucei from bloodstream into procyclic form requires neither intermediary nor short-stumpy stage. Mol. Biochem. Parasitol. 44, 261-270 (1991).
6. Bitonti, A. J., J. A. Dumont, P. P. McCann: Characterization of Trypanosoma brucei brucei S-adenosyl-L-methionine decarboxylase and its inhibition by Berenil, pentamidine and methylglyoxal bis(guanylhydrazone). Biochem. J. 237, 685-689 (1986).
7. Bradford, M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254 (1976).
8. Brun, R., L. Jenni, M. Schönenberger, K. F. Schell: In vitro cultivation of bloodstream forms of Trypanosoma brucei, T. rhodesiense, and T. gambiense. J. Protozool. 28, 470-479 (1981)
9. Byers, T. L., T. L. Bush, P. P. McCann, A. J. Bitonti: Antitrypanosomal effects of polyamine biosynthesis inhibitors correlate with increase in Trypanosoma brucei brucei S-adenosyl-L-methionine. Biochem. J. 274, 527-533 (1991).
10. Cross, G. A. M.: Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Parasitology 71, 393-417 (1975).
11. Czichos, J., C. Nonnengaesser, P. Overath: Trypanosoma brucei: cis-aconitate and temperature reduction as triggers of synchronus transformation of bloodstream to procyclic trypomastigotes in vitro. Exp. Parasitol. 62, 283-291 (1986).
12. Duszenko, M., M. A. J. Ferguson, G. S. Lamont, M. R. Rifkin, G. A. M. Cross: Cysteine eliminates the feeder cell requirement for cultivation of Trypanosoma brucei bloodstream forms in vitro. J. Exp. Med. 162, 1256-1263 (1985).
13. Duszenko, M., K. Muhlstädt, A. Broder: Cysteine is an essential growth factor for Trypanosoma brucei bloodstream forms. Mol. Biochem. Parasitol. 50, 269-274 (1992).
14. Fairlamb, A. H., G. B. Henderson, C. J. Bacchi, A. Cerami: In vivo effects of difluoromethylornithine on trypanothione and polyamine levels in bloodstream forms of Trypanosoma brucei. Mol. Biochem. Parasitol. 24, 185-191 (1987).
15. Fairlamb, A. H., A. Cerami: Metabolism and functions of trypanothione in the kinetoplastida. Annu. Rev. Microbiol. 46, 695-729 (1992).
16. Ghoda, L., T. Van Daalen Wetters, M. Macrae, D. Ascherman, P. Coffino: Prevention of rapid intracellular degradation of ODC by carboxyl-terminal truncation. Science 243, 1493-1495 (1989).
17. Ghoda, L., M. A. Phillips, K. E. Bass, C. C. Wang, P. Coffino: Trypanosome ornithine decarboxylase is stable because it lacks sequence found in the carboxyl terminus of the mouse enzyme which target the latter for intracellular degradation. J. Biol. Chem. 265, 11823-11826 (1990).
18. Ghoda, L., D. Sidney, M. Macrae, P. Coffino: Structural elements of ornithine decarboxylase required for intracellular degradation and polyamine-dependent regulation. Mol. Cell. Biol. 12, 2178-2185 (1992).
19. Giffin, B. F., P P McCann, A. L. Bitonti, C. J. Bacchi: Polyamine depletion following exposure to DL-α-difluoromethylornithine both in vivo and in vitro initiates morphology alterations and mitochondrial activation in a monomorphic strain of Trypanosoma brucei brucei. J Protozool. 33, 238-243 (1986).
20. Giffin, B. F., P. P. McCann: Physiological activation of the mitochondrion and the transformation capacity of DFMO induced intermediate and short stumpy bloodstream form trypanosomes. Am. J. Trop. Med. Hyg. 40, 487-493 (1989).
21. Hamm, B., A. Schindler, D. Mecke, M. Duszenko: Differentiation of Trypanosoma brucei bloodstream trypomastigotes from long slender to short stumpy-like forms in axenic culture. Mol. Biochem. Parasitol. 40, 13-22 (1990).
22. Heby, O., L. Persson: Molecular genetics of polyamine synthesis in eukaryotic cells. Trends Biochem. Sci. 15, 153-158 (1990).
23. Hesse, F., P. M. Selzer, K. Mühlstadt, M. Duszenko: A novel technique for long term maintenance of bloodstream form trypanosomes in vitro. Mol. Biochem. Parasitol. 70, 157-166 (1995).
24. Hirumi, H., J. J. Doyle, K. Hirumi: African trypanosomes: Cultivation of animal infective Trypanosoma brucei in vitro. Science 196, 992-994 (1977).
25. Pays, E., J. Hanocq-Quertier, F. Hanocq, S. Van Assel, D. Nolan, S. Rolin: Abrupt RNA changes precede the first cell division during the differentiation of Trypanosoma brucei bloodstream forms into procyclic forms in vitro. Mol. Biochem. Parasitol. 61, 107-114 (1993).
26. Pegg, A. E.: Investigation of the turnover of rat liver S-adenosylmethionine decarboxylase using a specific antibody. J. Biol. Chem. 254, 3249-3253 (1979).
27. Pegg, A. E.: Polyamine metabolism and its importance in neoplastic growth and as a target for chemotherapy. Cancer Res. 48, 759-774 (1988).
28. Pegg, A. E., H. Pösö: S-adenosylmethionine decarboxylase (rat liver). Methods Enzymol. 94, 234-239 (1983).
29. Pegg, A. E., P. P. McCann: S-adenosylmethionine decarboxylase as an enzyme target for therapy. Pharmacol. Ther. 56, 359-377 (1992).
30. Phillips, M. A., P. Coffino, C. C. Wang: Cloning and sequencing of ornithine decarboxylase gene from Trypanosoma brucei. J. Biol. Chem. 262, 8721-8727 (1987).
31. Rogers, S., R. Wells, M. Rechensteiner: Amino acid sequence common to rapidly degraded proteins: the PEST hypothesis. Science 234, 364-368 (1986).
32. Rolin, S., P. Paindavoine, J. Hanocq-Quertier, F. Hanocq, Y. Cales, D. Le Ray, P. Overath, E. Pays: Transient adenylate cyclase activation accompanies differentiation of Trypanosoma brucei bloodstream to procyclic forms. Mol. Biochem. Parasitol. 61, 115-126 (1993).
33. Seiler N.: Inhibition of enzymes oxidizing polyamines. In: P. P. McCann, A. E. Pegg, A. Sjoerdsma (eds.): Inhibition of Polyamine Metabolism. Biological significance and basis for new therapies. pp. 49-78. Academic Press. New York 1987.
34. Selzer, P. M., P. Webster, M. Duszenko: Influence of Ca2+ depletion on cytoskeleton and nucleolus morphology in Trypanosoma brucei. Eur. J. Cell Biol. 56, 104-112 (1991).
35. Tabor, C. W., H. Tabor: Polyamines. Annu. Rev. Biochem. 53, 749-790 (1984).
36. Tekwani, B. L., C. J. Bacchi, A. E. Pegg: Putrescine activated S-adenosylmethionine decarboxylase from Trypanosoma brucei brucei. Mol. Cell. Biochem. 117, 53-61 (1992).
37. Traore-Leroux, T. T. F. Fumoux, J. Chaize, G. E. Roelants: Trypanosoma brucei: Polyamine oxidase mediated trypanolytic activity in the serum of naturally resistant cattle. Exp. Parasitol. 64, 401-409 (1987).
38. Vickerman, K.: Developmental cycles and biology of pathogenic trypanosomes. Br. Med. Bull. 41, 105-114 (1985).
39. Wagner, J., C. Danzin, P. Mamont: Reversed-phase ion-pair liquid chromatographic procedure for the simultaneous analysis of S-adenosylmethionine, its metabolites and the natural polyamines. J. Chromatogr. 227, 349-368 (1982).
40. Ziegelbauer, K., M. Quinten, H. Schwarz, T. W. Pearson, P. Overath: Synchronous differentiation of Trypanosoma brucei from bloodstream to procyclic forms in vitro. Eur. J. Biochem. 192, 373-378 (1990).