A retrospective study of the relative utility of electrophoresis, immunoelectrophoresis, immunofixation, and nephelometry in the investigation of serum proteins

Clinical Biochemistry

Volumn 29, Issue 1, 1996, Pages 39-42

  Salkie, Mark L ^a  

^a UNIVERSITY OF ALBERTA HOSPITAL   (Canada)

Author keywords

electrophoresis; immunoelectrophoresis; immunofixation; monoclonal gammopathy; paraproteinemia; serum proteins

Indexed keywords

IMMUNOGLOBULIN; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; PARAPROTEIN; PLASMA PROTEIN;

ARTICLE; ELECTROPHORESIS; HUMAN; IMMUNOELECTROPHORESIS; MONOCLONAL IMMUNOGLOBULINEMIA; NEPHELOMETRY; PARAPROTEINEMIA; PRIORITY JOURNAL;

EID: 0030031676     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/0009-9120(95)02002-0     Document Type: Article

References (30)

1. Kohn J. A cellulose acetate supporting medium for zone electrophoresis. Clin Chim Acta 1957; 2: 297-303.
2. Jeppsson J-O, Laurell C-B, Franzén B. Agarose gel electrophoresis. Clin Chem 1979; 25: 629-38.
3. Grabar P, Williams CA. Méthode permettant l'étude conjugée des propriétés électrophorétiques et immunochimiques d'un mélange de protéines. Application au sérum sanguin. Biochim Biophys Acta 1953; 10: 193-4.
4. Alper CA, Johnson AM. Immunofixation electrophoresis: a technique for the study of protein polymorphysm. Vox Sang 1969; 17: 445-52.
5. Whicher JT, Warren C, Chambers RE. Immunochemical assays for immunoglobulins. Ann Clin Biochem 1984; 21: 78-91.
6. Salkie ML. Too many tests? Lancet 1971; ii: 915-6.
7. Laurell C-B. Electrophoresis, specific protein assays, or both in measurement of plasma proteins? Clin Chem 1973; 19: 99-102.
8. Heremans JF, Masson PL. Specific analysis of immunoglobulins. Techniques and clinical value. Clin Chem 1973; 19: 294-300.
9. Whicher JT, Calvin J, Riches P, Warren C. The laboratory investigation of paraproteinaemia. Ann Clin Biochem 1987; 24: 119-32.
10. Whicher JT, Spence CE. Serum protein zone electrophoresis - an outmoded test? Ann Clin Biochem 1987; 24: 133-9.
11. Keren DF, Warren JS, Lowe JB. Strategy to diagnose monoclonal gammopathies in serum: high-resolution electrophoresis, immunofixation, and κ/λ quantification. Clin Chem 1988; 34: 2196-201.
12. Cawley LP. Electrophoresis and Immunoelectrophoresis. Boston: Little, Brown and Company, 1969.
13. Aguzzi F, Jayakar SD, Merlini G, Petrini C. Electrophoresis: Cellulose acetate vs agarose gel, visual inspection vs densitometry. Clin Chem 1981; 27: 1944-45.
14. Kahn SN, Strony LP. Imprecision of quantification of serum protein fractions by electrophoresis on cellulose acetate. Clin Chem 1986; 32: 356-7.
15. Aguzzi F, Kohn J, Petrini C, Whicher JT. Densitometry of serum protein electrophoretograms. Clin Chem 1986; 32: 2004-5.
16. Schreiber WE, Chiang E, Tse SSL. Electrophoresis underestimates the concentration of polyclonal immunoglobulins in serum. Am J Clin Pathol 1992; 97: 610-3.
17. Zawadzki ZA, Edwards GA. Pseudoparaproteinemia due to hypertransferrinemia. Am J Clin Pathol 1979; 54: 802-9.
18. Aguzzi F, Petrini C, Gasparro C. Serum protein electrophoresis: Italian survey 1986. Ann Clin Biochem 1989; 26: 249-53.
19. Aguzzi F, Petrini C, Gasparro C. Serum protein electrophoresis: Italian survey 1986. Ann Clin Biochem 1990; 27: 90.
20. Vladutiu AO, Baitman LS. M-components at the application point on serum protein electrophoresis. Am J Clin Pathol 1982; 77: 509-11.
21. Pudek MR. Investigation of monoclonal gammopathies by immunoelectrophoresis and immunofixation. Clin Chem 1982; 28: 1231-2.
22. Pekelharing JM, Haije WG. Immunofixation cannot replace immunoelectrophoresis. Clin Chem 1984; 30: 174.
23. Salkie ML. Appearance of light chain disease in serum electrophoresis. Report of 11 cases. Ann Clin Lab Sci 1986; 16: 241-4.
24. Salkie ML. Observations on the electrophoretic and immunoelectrophoretic appearance of the prealbumin region in biological fluids. Ann Clin Lab Sci 1987; 17: 312-7.
25. Gadek GE, Hosea SW, Gelfand JA, Frank MM. C1 inhibitor phenotypes in hereditary angioedema: Genetic implications of successful danazol therapy. Clin Res 1977; 25: 357A.
26. Irjala K, Suonpää J, Laurent B. Identification of CSF leakage by immunofixation. Arch Otolaryngol 1979; 105: 447-8.
27. Whicher JR, Higginson J, Riches PG, Radford S. Clinical applications of immunofixation: detection and quantitation of complement activation. J Clin Pathol 1980; 33: 781-5.
28. Jobbs JR. Paraproteins, benign or malignant? Br Med J 1967; 3: 699-704.
29. Smith AM, Thompson RA. Paraprotein estimation: a comparison of immunochemical and densitometric techniques. J Clin Pathol 1978; 31: 1156-60.
30. Riches PG, Sheldon J, Smith AM, Hobbs JR. Overestimation of monoclonal immunoglobulin by immunochemical methods. Ann Clin Biochem 1991; 28: 253-9.