Conformation of the hypervariable region L3 without the key proline residue

Protein Science

Volumn 5, Issue 1, 1996, Pages 167-169

  Guarné, Alba ^a   Bravo, Jerónimo ^a   Calvo, Javier ^b   Lozano, Francisco ^b   Vives, Jordi ^b   Fita, Ignasi ^a  

^a UNIVERSITAT POLITÈCNICA DE CATALUNYA   (Spain)

^b HOSPITAL CLÍNIC   (Spain)

Author keywords

canonical forms; complementarity determining regions (CDRs); Fab fragment

Indexed keywords

IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN;

ARTICLE; CRYSTAL STRUCTURE; IMMUNOGLOBULIN STRUCTURE; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; STRUCTURE ANALYSIS;

MURINAE;

EID: 0030031169     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560050121     Document Type: Article

References (12)

1. Chothia C, Lesk AM. 1987. Canonical structures for the hypervariable regions of immunoglobulins. J Mol Biol 196:901-917.
2. Chothia C, Lesk AM, Tramontane A, Levitt M, Smith-Gill SJ, Air G, Sheriff S, Padlan EA, Davies D, Tulip WR. 1989. Conformations of immunoglobulin hypervariable regions. Nature 342:877-883.
3. Guarné A, Bravo J, Calvo J, Lozano F, Vives J, Fita I. 1995. Three-dimensional structure of a monoclonal antibody Fab fragment against CD5. An Quím 91:296-299.
4. Heinrichs A, Milstein C, Gherardi E. 1995. Universal cloning and direct sequencing of rearranged antibody V genes using C region primers, biotin-captured cDNA and one-side PCR. J Immunol 178:241-251.
5. Kabat EA. 1978. The structural basis of antibody complementarity. Adv Protein Chem 32:1-75.
6. Kabat EA, Wu TT, Bilofsky H. 1977. Unusual distributions of amino acids in complementarity-determining (hypervariable) segments of heavy and light chains of immunoglobulins and their possible roles in specificity of antibody-combining sites. J Biol Chem 252:6609-6616.
7. Kabat EA, Wu TT, Perry HM, Gottesman KS, Foeller C. 1991. Sequences of proteins of immunological interest, 5th ed. Bethesda, Maryland: National Institutes of Health.
8. Lozano F, Maertzdorf B, Pannell R, Milstein C. 1994. Low cytoplasmic mRNA levels of immunoglobulin κ light chain genes containing nonsense codons correlate with inefficient splicing. EMBO J 13:4617-4622.
9. Matthews BW. 1968. Solvent content of protein crystals. J Mol Biol 33: 491-497.
10. Sheriff S, Silverton EW, Padlan EA, Cohen GH, Smith-Gill SJ, Funzel BC, Davies DR. 1987. Three-dimensional structure of an antibody-antigen complex. Proc Natl Acad Sci USA 84:8075-8079.
11. Suh SW, Bhat TN, Navia MA, Cohen GH, Rao DN, Rudikoff S, Davies DR. 1986. The galactan-binding immunoglobulin Fab J539: An x-ray diffraction study at 2.6 Å resolution. Proteins Struct Funct Genet 1:74-80.
12. Tormo J, Blaas D, Parry NR, Rowlands D, Stuart D, Fita I. 1994. Crystal structure of a human rhinovirus neutralizing antibody complexed with a peptide derived from viral capsid protein VP2. EMBO J 13:2247-2256.