Comparison of HT29-18-C1 and Caco-2 cell lines as models for studying intestinal paracellular drug absorption

Pharmaceutical Research

Volumn 13, Issue 2, 1996, Pages 216-221

  Collett, Andrew ^a   Sims, Erika ^a   Walker, David ^a   He, Yan Ling ^a   Ayrton, John ^b   Rowland, Malcolm ^a   Warhurst, Geoffrey ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Caco 2; HT29 18 C1 cell lines; Paracellular drug absorption

Indexed keywords

ATENOLOL; CIMETIDINE; HYDROCHLOROTHIAZIDE; MANNITOL; ONDANSETRON; RANITIDINE;

ARTICLE; CELL LINE; COLON CARCINOMA; COLON MUCOSA; COMPARATIVE STUDY; DRUG PENETRATION; DRUG TRANSPORT; ELECTRIC RESISTANCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IN VITRO STUDY; INTESTINE ABSORPTION; MEMBRANE PERMEABILITY; PRIORITY JOURNAL;

ATENOLOL; BIOLOGICAL TRANSPORT; CACO-2 CELLS; CELL DIVISION; CELL MEMBRANE PERMEABILITY; CIMETIDINE; HT29 CELLS; HUMANS; HYDROCHLOROTHIAZIDE; INTESTINAL ABSORPTION; MANNITOL; RANITIDINE; TRITIUM;

EID: 0030030387     PISSN: 07248741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1016082829111     Document Type: Article

References (13)

1. P. Artursson. Cell culture as models for drug absorption across the intestinal mucosa. Crit. Revs. in Ther. Drug Systems 8:305-330 (1991).
2. R. A. Conradi, A. R. Hilgers, N. F. H. Ho and P. S. Burton. The influence of peptide structure on the transport across Caco-2 cells. Pharm. Res. 8:1453-1460 (1991).
3. G. Wilson, J. F. Hassam, C. J. Dix, I. Williamson, R. Shah, M. Mackay, and P. Artursson. Transport and permeability properties of human Caco-2 cells: An in vitro model of the intestinal epithelial cell barrier. J. Control. Release 11:25-40 (1990).
4. P. Artursson, A-L. Ungell and J-E Löfroth. Selective paracellular permeability in two models of intestinal absorption: cultured monolayers of human intestinal epithelial cells and rat intestinal segments. Pharm. Res. 10:1123-1129 (1993).
5. S. E. Crowe and M. H. Perdue. Anti-Immunoglobulin E-stimulated ion transport in human large and small intestine. Gastroenterology. 105:764-772 (1993).
6. P. Claude. Morphological factors influencing transepithelial permeability: A model of the resistance of the zonula occludens. J. Membrane Biol. 39:219-232 (1978).
7. C. Huet, C. Sahuquillo-Merino, E. Coudrier and D. Louvard. Absorptive and mucus-secreting subclones isolated from a multipotent intestinal cell line (HT-29) provide new models for cell polarity and terminal differentiation. J. Cell Biol. 105:345-357 (1987).
8. 1 intestinal cells: a new model for studying the epithelial transport of drugs. Biochim. et Biophys. Acta. 1177:134-138 (1993).
9. I. J. Hiadalgo, T. J. Raub and R. T. Borchardt. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology. 96:736-749 (1989).
10. J. H. Sellin and R. DeSoignie. Ion transport in human colon in vitro. Gastroenterology. 93:441-448 (1987).
11. L. S. Gan, P.H. Hsyu, J. F. Pritchard and D. Thakker. Mechanism of intestinal absorption of ranitidine and ondansetron: transport across Caco-2 cell monolayers. Pharm. Res. 10:1722-1725 (1993).
12. H. Lennernäs, Ö. Ahrenstedt and A-L. Ungell. Intestinal drug absorption during induced net water absorption in man; a mechanistic study using antipyrine, atenolol and enalaprilat. Br. J. Clin. Pharmacol. 37:587-596 (1994).
13. F. A. Sutcliffe, S. A. Riley, B. Kaser-Liard, L. A. Turnberg and M. Rowland. Absorption of drugs from human jejunum and ileum. Br. J. Clin. Pharmacol. 26:206-207P (1988).