A general method of analysis of ligand binding to competing macromolecules using the spectroscopic signal originating from a reference macromolecule. Application to Escherichia coli replicative helicase DnaB protein-nucleic acid interactions

Biochemistry

Volumn 35, Issue 7, 1996, Pages 2117-2128

  Jezewska, Maria J ^a   Bujalowski, Wlodzimierz ^a  

^a UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; HELICASE; NUCLEIC ACID;

ARTICLE; COMPUTER SIMULATION; DNA REPLICATION; ESCHERICHIA COLI; FLUORESCENCE; LIGAND BINDING; MACROMOLECULE; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; SPECTROSCOPY; STOICHIOMETRY; THERMODYNAMICS;

BACTERIAL PROTEINS; BINDING, COMPETITIVE; DNA HELICASES; DNAB HELICASES; ESCHERICHIA COLI; FLUORESCENCE POLARIZATION; LIGANDS; POLYNUCLEOTIDES; PROTEIN BINDING; SPECTROMETRY, FLUORESCENCE; THERMODYNAMICS;

EID: 0030030309     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi952344l     Document Type: Article

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