A nontoxic cholera enterotoxin (CT) analog is chimeric with regard to both epitypes of CT-B subunits, CT-B-1 and CT-B-2

Infection and Immunity

Volumn 64, Issue 1, 1996, Pages 346-348

  Boesman Finkelstein, Mary ^a   Peterson, Johnny W ^b   Thai, Louise S ^a   Finkelstein, Richard A ^a  

^a University of Missouri   (United States)

^b University of Texas Medical Branch School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHIMERIC PROTEIN; CHOLERA TOXIN; CHOLERA TOXIN B SUBUNIT; CHOLERA VACCINE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; RECOMBINANT PROTEIN;

ADENOSINE DIPHOSPHATE RIBOSYLATION; ANIMAL CELL; ARTICLE; BACTERIUM MUTANT; CONTROLLED STUDY; GENETIC ENGINEERING; IMMUNOBLOTTING; MICROBIAL ATTENUATION; NONHUMAN; PRIORITY JOURNAL; RABBIT; TOXIN STRUCTURE; VIBRIO CHOLERAE;

EID: 0030030016     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.1.346-348.1996     Document Type: Article

References (19)

1. Booth. B. A., M. Boesman-Finkelstein, and R. A. Finkelstein. 1984 Vibrio cholerae hemagglutinin protease nicks cholera enterotoxin Infect, Immun. 43:558-560.
2. Burnette, W. N. 1981. "Western blotting" electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A Anal. Biochem 112:195-203
3. Clemens, J., D. A. Sack, J. R. Harris, F. van Loon, J. Chakraborty, F. Ahmed, M. R. Rao, M. R. Khan, M. D. Yunus, N. Huda, B. F. Stanton, B. A. Kay, S. Walter, R. Eeckels. A.-M. Svennerholm, and J. HoImgren. 1990 Field trial of oral cholera vaccines in Bangladesh: results from three-year follow-up. Lancet 335:270-273
4. Cunningham, J., and R. A, Finkelstein. 1993 and 1994 Unpublished observations.
5. Finkelstein, R. A., M. L. Vasil, and R. K. Holmes. 1974. Studies on toxmo genesis in Vibrio cholerae. I Isolation of mutants with altered toxinogenicity. J Infect. Dis. 129:117-123.
6. Finkelstein. R. A., F. Burks, A. Zupan, W. S. Dallas. C. O. Jacob, and D. S. Ludwig. 1987 Epitopes of the cholera family of enteroloxins. Rev. Infect. Dis. 9:544-561.
7. Finkelstein, R. A., and J. J. LoSpalluto. 1969. Pathogenesis of experimental cholera preparation and isolation of choleragen and choleragenoid. J. Exp. Med. 130:185-202.
8. Finkelstein, R. A., K. L. Mackey, R. S. Newsham, and M. F. Burks. 1988 Epitypes of the cholera and related enterotoxms, abstr B103, p 46 In Abstracts of the 88th Annual Meeting of the American Society for Microbiology 1988. American Society for Microbiology. Washington, D.C.
9. Fontana. M., R. Manetti. V.Glannelli, C. Magagnoli. A. Marchini. R. Olivieri, M. Domenighini. R. Rappuoli. and M, Pizza. 1995. Construction of nontoxic derivatives of cholera toxin and characterisation of the immunological response against the A subunit. Infect Immun. 63:2356-2360.
10. Fujita, K., and R. A. Finkelstein. 1972. Antitoxic immunity in experimental cholera: comparison of immunity induced perorally and parentcrally in mice J. Infect. Dis 125:647-655.
11. Gupta, R. K., S. C. Szu, R. A. Finkelstein, and J. B. Ruhbins. 1992 Synthesis, characterisation, and some immunologic properties of conjugates composed of the detoxified hpopolysaccharide of Vibrio cholerue O1 serotype Inaba bound to cholera toxin Infect. Immun 60:3201-3208.
12. Hase, C. C., L. S. Thai, M. Boesman-Finkelstein. V. L. Mar, W. N. Burnette, H. R. Kaslow, L. A. Stevens, J. Moss, and R. A. Finkelstein. 1994 Construetion and characterization of recombmant Vibrio choleiae strains producing inactive cholera toxin analogs. Infect Immun. 62:3051-3057.
13. Kaper, J. B., J. G. Morris, Jr., and M. M. Levine. 1995. Cholera. Clin. Microbiol. Rev. 8:48-86.
14. Kazemi, M., and R. A. Finkelstein. 1990. Study of cpitopes of cholera enterotoxin-reiated enterotoxins using checkerboard irnmunoblottmg. Infect. Immun 58:2352-2360.
15. Marchlewicz, B. A., and R. A. Finkelstein 1983 Immunologic differences among the cholera/coli family of enterotoxms Diagn. Microbiol. Infect. Dis. 1:129-138.
16. Pierce, N. F., W. C. Cray, Jr., J. B. Sacci, Jr., J. P. Craig, R. Germanier, and E. Fürer. 1983. Procholeragenoid: a safe and effective antigen for oral immunization against experimental cholera. Infect. Immun. 40:1112-1118.
17. Qu, Z.-H., and R. A. Finkelstein. 1993 Checkerboard immunoblotting recognizes twenty epilopes among the B subunit proteins of the cholera entcrotoxin family. Electrophoresis 14:899-901.
18. Rappaport, R. S., B. A. Rubin, and H. Tint. 1974. Development of purified cholera toxoid. I. Purification of toxin. Infect. Immun. 9:294-303
19. Uesaka, Y., Y. Otsuka, Z. Lin. S. Yamasahi, J. Yamaoka, H. Kurazono, and Y. Takeda. 1994 Simple method of purification of Estherichia coli heat-labile enterotoxin and cholera toxin using immobilized galactose Microbiol Pathol. 16:71-76.