Identification of stress proteins in lysates of human cell lines separated by two-dimensional electrophoresis and electroblotted simultaneously onto two different membranes

Electrophoresis

Volumn 17, Issue 5, 1996, Pages 892-898

  Vogt, Christian P ^a   Willi, Annemarie ^a   Hess, Daniel ^a,b   Hunziker, Peter E ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH   (Switzerland)

Author keywords

Amino acid sequence analysis; Electroblotting; Liquid chromatography; Mass spectrometry; Peptide mass fingerprinting; Two dimensional polyacrylamide gel electrophoresis

Indexed keywords

AMINO ACIDS; CELL CULTURE; ELECTROPHORESIS; GELS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MEMBRANES; PEPTIDES;

AMINO ACID SEQUENCE ANALYSIS; CATIONICS; ELECTROBLOTTING; HUMAN CELL LINES; N-TERMINAL SEQUENCES; P.V.D.F; PEPTIDE MASS FINGERPRINTING; SEQUENCE ANALYSIS; STRESS PROTEIN; TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS;

MASS SPECTROMETRY;

HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 60; HEAT SHOCK PROTEIN 70;

CELL CULTURE; CELL LYSATE; CONFERENCE PAPER; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; LIVER CELL; MASS SPECTROMETRY; MOLECULAR WEIGHT; PEPTIDE MAPPING; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; TECHNIQUE;

EID: 0030029824     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150170510     Document Type: Conference Paper

References (21)

1. Kägi, J. H. R., Methods Enzymol. 1991, 205, 613-626.
2. Lindquist, S., Annu. Rev. Biochem., 1986, 55, 1151-1191.
3. Welch, W. J., in: Morimoto, R. I., Tissieres, A., Georgopoulos, C. (Eds.), Stress Proteins in Biology and Medicine, Cold Spring Harbor Laboratory Press 1990, pp. 223-278.
4. Wan, M., Kägi, J. H. R., Hunziker, P. E., Protein Expr. Purif. 1993, 4, 38-44.
5. Hunziker, P. E., Kägi, J. H. R., Biochem. J. 1985, 231, 375-382.
6. O'Farrell, P. H., J. Biol. Chem. 1975, 250, 4007-4021.
7. Görg, A., Postel, W., Siegfried, G., Eleclrophoresis 1988, 9, 531-546.
8. Hochstrasser, D. F., Harrington, M. G., Hochstrasser, A.-C, Miller, M. J., Merril, C. R., Anal. Biochem. 1988, 173, 424-435.
9. Bjellqvist, B., Sanchez, J.-C., Pasquali, C., Ravier, F., Paqeut, N., Frutiger, S., Hughes, G. J., Hochstrasser, D., Electrophoresis 1993, 14, 1375-1378.
10. Matsudaira, P., J. Biol. Chem. 1987, 262, 10035-10038.
11. Brown, J. L., Roberts, W. K., J. Biol. Chem. 1976, 254, 525-529.
12. Pappin, D. J. C., Hojrup, P., Bleasby, A. J., Curr. Biol. 1993, 3, 327-332.
13. James, P., Quadroni, M., Carafoli, E., Gonnet, G., Biochem. Biophys. Res. Comm. 1993, 195, 58-64.
14. Fernandez, J., DeMott, M., Atherton, D., Mische, S. M., Anal. Biochem. 1992, 201, 255-264.
15. Aebersold, R., Patterson, S. D., Hess, D., in: Angeletti, R. H. (Ed.), Techniques in Protein Chemistry III, Academic Press, San Diego 1992, pp. 87-96.
16. Patterson, S. D., Hess, D., Yungwirth, T. Aebersold, R., Anal. Biochem. 1992, 202, 193-203.
17. Hess, D., Covey, T. C., Winz, R., Brownsey, R. W., Aebersold, R., Protein Sci. 1993, 2, 1342-1351.
18. Vandekerckhove, J., Rider, M., Rasmussen, H.-H., De Boek, S., Puype, M., Van Damme, J., Gesser, B., Celis, J., in: Imahori, K., Sakiyama, F. (Eds.), Methods in Protein Sequence Analysis, Plenum Press, New York 1993, pp. 11-19.
19. Rider, M. H., Puype, M., Van Damme, J., Gevaert, K., De Boeck, S., D'Alayer, J., Rasmussen, H. H., Celis, J. E., Vandekerckhove, J., Eur. J. Biochem. 1995, 230, 258-265.
20. Sanchez, J.-C., Ravier, F., Pasquali, C., Frutiger, S., Paquet, N., Bjellqvist, B., Hochstrasser, D. F., Hughes, G. J., Electrophoresis 1992, 13, 715-717.
21. Waldinger, D., Eckerskorn, C., Lottspeich, F., Cleve, H., Biol. Chem. Hoppe-Seyler 1988, 369, 1185-1189.