Detection of microinjected genes in bovine preimplantation embryos with combined DNA digestion and polymerase chain reaction

Molecular Reproduction and Development

Volumn 43, Issue 2, 1996, Pages 150-157

  Hyttinen, Juha Matti ^a   Peura, Teija ^a,b   Tolvanen, Minna ^a   Aalto, Jussi ^a   Jänne, Juhani ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

^b MONASH MEDICAL CENTRE   (Australia)

Author keywords

Cattle; Detection; Embryo; PCR; Sexing; Transgene

Indexed keywords

PROTEINASE;

ANIMAL CELL; ARTICLE; CATTLE; DNA DETERMINATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEX DETERMINATION; TRANSGENE; Y CHROMOSOME;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BLASTOCYST; CATTLE; FEMALE; GENE TRANSFER TECHNIQUES; POLYMERASE CHAIN REACTION; PREGNANCY; SEX DETERMINATION (ANALYSIS);

EID: 0030028823     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2795(199602)43:2<150::AID-MRD3>3.0.CO;2-Q     Document Type: Article

References (39)

1. Bavister BD (1992): Co-culture for embryo development: Is it really necessary? Hum Reprod 7:1339-1341.
2. Bishop JO, Smith P (1989): Mechanism of chromosomal integration of microinjected DNA. Mol Biol Med 6:283-298.
3. Bowen RA, Reed ML, Schnieke A, Seidel Jr GE. Stacey A, Thomas WK, Kajikawa O (1994): Transgenic cattle resulting from biopsied embryos: Expression of c-ski in a transgenic calf. Biol Reprod 50: 664-668.
4. Burdon TG, Wall RJ (1992): Fate of microinjected genes in preimplantation mouse embryos. Mol Reprod Dev 33.436-442.
5. Costa ND, Thacker J (1992): The efficiency of restriction endonuclease digests determined by PCR. Biotechniques 13:190.
6. Cousens C, Carver AS, Wilmut I, Colman A, Gamer I, O'Neill GT (1994): Use of PCR-based methods for selection of integrated transgenes in preimplantation embryos. Mol Reprod Dev 39:384-391.
7. Erlich HA, Gelfand D, Sninsky JJ (1991): Recent advances in the polymerase chain reaction. Science 252:1643-1651.
8. Eyestone WH, First NL (1989): Co-culture of early cattle embryos to the blastocyst stage with oviducal tissue or in conditioned medium. J Reprod Fertil 85:715-720.
9. Hamada T, Sasaki H, Seki R. Sakaki Y (1993): Mechanism of chromosomal integration of transgenes in microinjected mouse eggs: Sequence analysis of genome-transgene and transgene-transgene junctions at two loci. Gene 128:197-202.
10. Hill KG, Curry J, DeMayo FJ, Jones-Diller K, Slapak JR, Bondioli KR (1992): Production of transgenic cattle by pronuclear injection. Theriogenology 37:222.
11. Horvat S, Medrano JF, Behboodi E, Anderson GB, Murray JD (1993): Sexing and detection of gene construct in microinjected bovine blastocysts using the polymerase chain reaction. Transgenic Res 2:134-140.
12. Hyttinen J-M, Peura T, Tolvanen M, Aalto J, Alhonen L, Sinervirta R, Halmekytö M, Myohänen S, Jänne J (1994): Generation of transgenic diary cattle from transgene-analyzed and sexed embryos produced in vitro. Biotechnology 12:606-608.
13. Jänne J, Hyttinen J-M, Peura T, Tolvanen M, Alhonen L, Sinervirta R, Halmekytö M (1994): Transgenic biorectors. Int J Biochem 26: 859-870.
14. Kafri T, Gao X, Razin A (1993): Mechanistic aspects of genome-wide demethylation in the preimplantation mouse embryo. Proc Natl Acad Sci USA 90:10558-10562.
15. King D, Wall RJ (1988): Identification of specific gene sequences in preimplantation embryos by genomic amplification: Detection of a transgene. Mol Reprod Dev 1:57-62.
16. Koczan D, Hobom G, Seyfert H-M (1991): Genomic organization of the bovine alpha-S1 casein gene. Nucleic Acids Res 19:5591-5596.
17. Krimpenfort P, Rademakers A, Eyestone W, van der Schans A, van den Broek S, Kooiman P, Kootwijk E, Platenburg G, Pieper F, Strijker R, de Boer H (1991): Generation of transgenic dairy cattle using "in vitro" embryo production. Biotechnology 9:844-847.
18. Lewis-Williams J, Houseal T, Harvey M, DiTullio P, Ziomek C (1993): Selection of transgenic preimplantation mouse embryos using fluorescence in situ hybridization. Theriogenology 39:258.
19. Lin F-K, Suggs S, Lin C-H, Browne JK, Smalling R, Egrie JC, Chen KK, Fox GM, Martin F, Stabinsky Z, Badrawi SM, Lai P-H, Goldwasser E (1995): Cloning and expression of the human erythropoietin gene. Proc Natl Acad Sci USA 82:7580-7584.
20. Ninomiya T, Hoshi M, Mizuno A, Nagao M, Yuki A (1989): Selection of mouse preimplantation embryos carrying exogenous DNA by polymerase chain reaction. Mol Reprod Dev 1:242-248.
21. Pääbo S, Irwin DM, Wilson AC (1990): DNA damage promotes jumping between templates during enzymatic amplification. J Biol Chem 265:4718-4721.
22. Paroush Z, Keshet I, Yisraeli J, Cedar H (1990): Dynamics of demethylation and activation of the α-actin gene in myoblasts. Cell 63: 1229-1237.
23. Parrish J-J, Susko-Parrish J, Winer MA, First NL (1988): Capacitation of bovine sperm by heparin. Biol Reprod 38:1171-1180.
24. Peden KWC, Pipas JM, Pearson-White S, Nathans D (1980): Isolation of mutants of an animal virus in bacteria. Science 209:1392-1396.
25. Peura TT, Hyttinen J-M, Tolvanen M, Jänne J (1994): Effects of microinjection-related treatments on the subsequent development of in vitro-produced bovine oocytes. Theriogenology 42:433-443.
26. Peura TT, Tolvanen M, Hyttinen J-M, Jänne J (1995): Effects of membrane piercing and the type of pronuclear injection fluid on development of in vitro-produced bovine embryos. Theriogenology 43:1087-1096.
27. Phi-Van L, Strätling WH (1988): The matrix attachment regions of the chicken lysozyme gene co-map with the boundaries of the chromatin domain. EMBO J 7:655-664.
28. Powell DJ, Galli G, Moor RM (1992): The fate of DNA injected into mammalian oocytes and zygotes at different stages of the cell cycle. J Reprod Fertil 95:211-220.
29. Reed KC, Matthaei KI, Jones MA (1988): Sex determination in ruminants using Y-chromosome specific polynucleotides. Patent Cooperation Treaty No WO 88.01300.
30. Rosenkrans CFJ, First NL (1991): Culture of bovine zygotes to the blastocyst stage: Effects of amino acids and vitamins. Theriogenology 35:266.
31. Sandberg G, Guhl E, Graessmann M, Graessmann A (1991): After microinjection hemimethylated DNA is converted into symmetrically methylated DNA before DNA replication. FEBS Lett 283:247-250.
32. Takeda S, Toyoda Y (1991): Expression of SV40-lacZ gene in mouse preimplantation embryos after pronuclear microinjection. Mol Reprod Dev 30:90-94.
33. Thomas WK, Schnieke A, Seidel GE (1993). Methods for producing transgenic bovine embryos from in vitro matured and fertilized oocytes. Theriogenology 40:679-688.
34. Vassilev LT, DePamphilis ML (1992): Guide to identification of origins of DNA replication in eucaryotic cell chromosomes. Crit Rev Biochem Mol Biol 27:445-472.
35. Wall RJ, Seidel GE (1992): Transgenic farm animals: A critical analysis. Theriogenology 38:337-357.
36. Wall RJ, Hawk HW, Nel N (1992): Making transgenic livestock: Genetic engineering on a large scale. J Cell Biochem 49:113-120.
37. 0 transgenic mice are derived from mosaic embryos. Transgenic Res 2:29-32.
38. Wilmut I, Clark AJ (1991): Basic techniques for transgenesis. J Reprod Feril Suppl 43:265-275.
39. Wirak DO, Chalifour LE, Wassarman PM, Muller WJ, Hasseil JA, DePamphilis ML (1985): Sequence dependent DNA replication in preimplantation mouse embryos. Mol Cell Biol 5:2924-2935.