In hepatocellular carcinoma AgNOR protein expression correlates with tumour mass doubling time

Journal of Hepatology

Volumn 24, Issue 1, 1996, Pages 60-65

  Trerè, Davide ^a   Gramantieri, Laura ^b   Siringo, Sebastiano ^b   Melchiorri, Cinzia ^b   Barbara, Luigi ^b   Bolondi, Luigi ^b   Derenzini, Massimo ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

AgNOR proteins; Doubling time; Hepatocellular carcinoma; Proliferation; Ultrasound examination

Indexed keywords

ADULT; AGED; ARTICLE; CELL PROLIFERATION; CLINICAL ARTICLE; CONTROLLED STUDY; ECHOGRAPHY; FEMALE; HISTOLOGY; HUMAN; HUMAN TISSUE; LIVER BIOPSY; LIVER CELL CARCINOMA; MALE; PRIORITY JOURNAL; TUMOR VOLUME;

EID: 0030027744     PISSN: 01688278     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-8278(96)80187-5     Document Type: Article

References (40)

1. Heitz E. Die Ursache der Gesetzmaissgen Zahl, Lage, Form und Grosse Pflanzlicher Nukleolen. Planta 1931; 12: 775-844.
2. McClintock DJL. The relation of a particular chromosomal element to the development of the nucleoli in Zea mays. Z Zellforsch Mikrosk Anat 1934; 21: 294-328.
3. Gall JG, Pardue ML. Nucleic acid part D. In: Grossman L, Moldave K, eds. Methods in Enzymology. New York: Academic Press, 1971; 470-80.
4. Gall JG, Pardue ML. Molecular hybridization of radioactive DNA to the DNA of cytological preparation. Proc Natl Acad Sci 1969; 64: 600-4.
5. Howell WM. Selective staining of nucleolus organizer regions (NORs). In: Bush H, Rothblum L, eds. The Cell Nucleus. New York: Academic Press, 1982: 89-142.
6. Derenzini M, Thiry M, Goessens G. Ultrastructural cytochemistry of the mammalian cell nucleolus. J Histochem Cytochem 1990; 38: 1237-56.
7. Goessens G. Nucleolar structures. Int Rev Cytol 1984; 84; 107-58.
8. Hernandez-Verdun D. Structural organization of the nucleolus in mammalian cells. Methods Achiev Exp Pathol 1986; 12: 26-62.
9. Derenzini M, Ploton D. Interphase nucleolar organizer regions in cancer cells. Int Rev Exp Pathol 1991; 32: 149-92.
10. Ploton D, Maneger M, Jeannesson P, Himber G, Pigeon F, Adnet JJ. Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer regions at the optical level. Histochem J 1986; 18: 5-14.
11. Trere' D, Migaldi M, Trentin GP. Higher reproducibility of morphometric analysis of the counting method for interphase AgNOR quantification. Anal Cell Pathol 1995; 8: 57-65.
12. Derenzini M, Ploton D. Interphase nucleolar organizer regions. In: Crocker J, ed. Molecular Biology in Histopathology. John Wiley and Sons, 1994: 1231-49.
13. Derenzini M, Trerè D. Importance of interphase nucleolar organizer regions in tumor pathology. Virchows Arch Cell Pathol B 1991; 61: 1-8.
14. Derenzini M, Sirri V, Trerè D. Nucleolar organizer regions in tumor cells. J Cancer 1994; 7: 71-7.
15. Colombo M, De Franchis R, Del Ninno E, Sangiovanni A, De Fazio C, Tommasini M, Donato MF, Piva A, Di Carlo V, Dioguardi N. Hepatocellular carcinoma in Italian patients with cirrhosis. N Engl J Med 1991; 325: 675-80.
16. Barbara L, Benzi G, Gaiani S, Fusconi F, Zironi G, Siringo S, Rigamonti A, Barbara C, Grigioni W, Maziotti A, Bolondi L. Natural history of untreated hepatocellular carcinoma in cirrhosis: a multivariate analysis of prognostic factors of tumor growth rate and patient survival. Hepatology 1992; 16: 132-7.
17. Calvet X, Bruix J, Gines P, Bru C, Vilana R, Rodes J. Prognostic factors in hepatocellular carcinoma in the West. A multivariate analysis in 206 patients. Histopathology 1990; 12: 735-60.
18. Hall PA, Levison DD. Assessment of cellular proliferation in histological material. J Clin Pathol 1990; 43: 184-92.
19. Nakano T, Oka K. Differential values of Ki 67 index and mitotic index of proliferating cell population. Cancer 1993; 15: 2401-8.
20. Edmondson HA, Steiner PE. Primary carcinoma of the liver: a study on 100 cases among 489 000 necropsies. Cancer 1954: 7: 462-503.
21. Collins WP, Loeffler RK, Tivey H. Observation of growth rates of human tumors. Am J Roentgenol 1956; 76: 988-1000.
22. Schwartz M. A biomathematical approach to clinical tumor growth. Cancer 1961; 14: 1272-94.
23. Derenzini M, Trerè D, Olivieri E, David E, Colombatto P, Bonino F, Brunetto MR. Is high AgNOR quantity in hepatocytes associated with increased risk of hepatocellular carcinoma in chronic liver disease? J Clin Pathol 1993; 46: 727-9.
24. Derenzini M, Romagnoli T, Caccarelli C, Eusebi V. Fixatives and silver stainability of NOR proteins at the light microscopic level. J Histochem Cytochem 1988; 36: 1453-4.
25. Smith PJ, Skilbeck H, Harrison A, Crocker J. The effect of a series of fixatives on the AgNOR technique. J Pathol 1988; 155: 109-12.
26. Crocker J, Macartney LC, Smith PJ. Correlation between DNA flow cytometry and nucleolar organizer region in non-Hogkin's lymphomas. J Pathol 1988; 151: 51-6.
27. Derenzini M, Pession A, Farabegoli F, Trerè D, Badiali M, Dehan P. Relationship between interphasic nucleolar organizer regions and growth rate in two neuroblastoma cell lines. Am J Pathol 1989; 134: 925-32.
28. Trerè D, Pession A, Derenzini M. The silver-stained proteins of nucleolar organizer regions as a parameter of cell duplication rate. Exp Cell Res 1989; 184: 131-7.
29. Derenzini M, Pession A, Trerè D. The quantity of nucleolar silver-stained proteins is related to proliferating activity in cancer cells. Lab Invest 1990; 63: 137-40.
30. Ofner D, Hittmair A, Marth C, Ofner C, Totsch M, Daxenhichler G, Mikuz G, Margreiter R, Schmid KW. Relationship between quantity of silver stained nucleolar organizer region associate proteins (AgNORs) and population doubling time in 10 breast cancer cell lines. Pathol Res Pract 1992; 188: 742-6.
31. Ogura S, Abe S, Sukoh N, Kunikane H, Nakajiama I, Inoue K, Kawakami Y. Corrrelation between nucleolar organizer regions visualized by silver staining and the growth rate of lung adenocarcinoma. Cancer 1992; 70: 63-8.
32. Kitamoto M, Nakanishi T, Kira S, Kawaguchi M, Nakashio R, Suemori S, Kajiyama G, Asahara T, Dohi K. The assessment of proliferating cell nuclear antigen in small hepatocellular carcinoma and its relationship to histologic characteristics and prognosis. Cancer 1993; 72: 1859-65.
33. Tarao K, Shimizu A, Harada M, Ohkawa S, Okamoto N, Kuni Y, Ito Y, Tamai S, Iimori K, Sugimasa I, Takemiya S, Okamoto T, Inoue T, Kanisawa M. In vitro uptake of bromodeoxyuridine by human hepatocellular carcinoma and its relationship to histologic finding and biological behavior. Cancer 1991; 68: 1789-94.
34. Tubiana M, Courdi A. Cell proliferation kinetics in human tumors: relation to probability of metastasis, dissemination and long term survival. Radiother Oncol 1989; 15: 1-18.
35. Okazaki N, Yoshino M, Yoshida T, Suzuki M, Moriyama N, Taakayasu K, Makuuchi M, Yamazaki S, Hasegawa H, Noguchi M, Hirohashi S. Evaluation of the prognosis for small hepatocellular carcinoma based on tumor volume doubling time. Cancer 1989; 63: 2207-10.
36. Grigioni W, Garbisia S, D'Errico A, Baccarini P, Liotta LA, Mancini AM. Evaluation of hepatocellular carcinoma aggressiveness by a panel of extracellular matrix antigens. Am J Pathol 1991; 138: 647-55.
37. Silverstrini R, Costa A, Regalia E. Cell kinetics study of hepatocellular carcinoma. Ital J Gastroenterol 1992; 24: 43-5.
38. Ebara M, Ohto M, Shiragawa T, Sugiura N, Kimura K, Matsutani S, Morita M, Saisho H, Tsuchiya Y, Okuda K. Natural history of minute hepatocellular carcinomas smaller than 3 cm complicating cirrhosis. Gastroenterology 1986; 90: 289-98.
39. Trerè D. Critical analysis of the methods commonly employed in the assessment of cell proliferation: advantages of the NOR silver-staining technique in routine cyto-histopathology. Anal Cell Pathol 1993; 5: 191-201.
40. Ofner B, Bankflavi A, Riehemann K, Bier B, Bocker W, Schmid KW. Wet autoclave pretreatment improves visualization of silver-stained nucleolar organizer region-associated proteins in routinely formalin-fixed and paraffin-embedded tissues. Mod Pathol 1994; 7: 946-50.